Part Collection

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Basic Parts

Registry-ID Name Functional description
BBa_25IQCJ3B ssDNA-mutation optimized mCherry-binding Repebody Encodes for an mCherry binding Repebody. Codon optimized for E. coli expression.
BBa_25X0LA8K mCherry-binding Repebody Encodes for an mCherry binding Repebody. Codon optimized for E. coli expression.
BBa_255DUKTY EGFP Encodes for enhanced green fluorescent protein (eGFP).
BBa_25GIIWIC HA-tag Encodes for an HA-tag bound by anti-HA antibodies. Used in purification and analysis. Codon optimized for expression in E. coli. (Sequence from pET303-mCherry-HA)
BBa_251PEVCU p62-wt Encodes for mammalian p62-wt protein, used in plasmid pRB90 for mammalian expression, and in pET-p62-linker-10xHis for binding assay from E. coli cells (from pRB90 plasmid)
BBa_25XLDO17 A1E Encodes for p62 nanobody binding UBA domain, used in RB-degrader
BBa_25VR4F6X A1E Encodes for p62 nanobody binding UBA domain, used in GBP-degrader and pET303
BBa_25GN544F GBP GFP-binding protein
BBa_25CP1ZSR mGBP mutated, non-binding version of GBP
BBa_25W7UMTC EGFP[NdeI-] EGFP, where a endogenous restriction site was removed.
BBa_25735N2C mCherry[NcoI-] mCherry, where a endogenous restriction site was removed.
BBa_25AGAPAR GGS3 A linker between components on the same plasmid to increase flexibility.
BBa_25VFZII5 T25 B. pertussis adenylate cyclase subunit Encodes for the catalytic site of the B. pertussis adenylate cyclase.
BBa_25AWKONG T18 B. pertussis adenylate cyclase subunit Encodes for the catalytic site of the B. pertussis adenylate cyclase.
BBa_258OKG9H mTagBFP2 Fluorescent protein, used in RB-A1E and RB-HA
BBa_256HJCS7 mTagBFP2 Fluorescent protein, from pET303-mTagBFP2-TEV-10xHis, in (m)GBP-degraders and for gel filtration binding assay
BBa_25TWO90U mTagBFP2 Fluorescent protein, used in pHM190, pHM193, and pHM135
BBa_25P2HPSC GST GST, affinity tag binding glutathione, can be used for purification and detection
BBa_25RSWBY3 Plasmid internal targeting Retron pms_366 editing retron
BBa_25DCBAZ7 LRRV targeting Retron 1 Repebody editing retron
BBa_254TXZJ8 LRRV targeting Retron 2 Repebody editing retron
BBa_259SUSLT mTagBFP2 Fluorescent protein, used pmTagBFP2 for simple cytosolic expression in mammalian cells
BBa_2542SOK3 FIS1 Encodes Mitochondrial fission 1 protein (FIS1), a component of the mitochondrial complex that promotes mitochondrial fission.

Composite Parts

Registry-ID Name Functional description
BBa_25T0IA49 EGFP - HA-tag Encodes for enhanced green fluorescent protein (eGFP). C-terminal HA-tag added for analysis.
BBa_25GR2JDM mCherry - HA-tag Encodes for red fluorescent protein (mCherry). C-terminal HA-tag added for analysis.
BBa_253J3LF3 mCherry-binding Repebody-4GGS-mTagBFP2-4GGS-HA-A1E Repebody degrader
BBa_2558RZ0V mCherry-binding Repebody-4GGS-mTagBFP2-HA Non-binding negative control for the Repebody-degrader
BBa_25WLKZDM EGFP[NdeI-]-HATag-’tgataa' HATagged EGFP for pCDFDuet MCS activity testing
BBa_25F2CTKF mCherry[NcoI-]-HisTag-’tgataa' HisTagged mCherry for pCDFDuet MCS activity testing
BBa_25A9293O Repebody[mutopt]-HisTag-’tgataa' His tagged mutation-optimized Repebody for pCDFDuetIso expression
BBa_25H9D35L mCherry-binding_Repebody - 6xHis - GGS3 - T25 The T25 fragment of adenylate cyclase fused to the mCherry-binding Repebody using a GGS3 - linker for increased flexibility. When the Repebody binds mCherry fused to the other fragment, the two subunits start a mechanism that indicates if the BACTH system is working. The His-Tag is for experimental validation.
BBa_255QHYDE mCherry-binding_Repebody - 6xHis - T25 The T25 fragment of adenylate cyclase fused to the mCherry-binding Repebody. When the Repebody binds mCherry bound to the other fragment, the two subunits start a mechanism that indicates if the BACTH system is working. The His-Tag is for experimental validation.
BBa_25L4RPGJ ssDNA-optimized-mCherry-binding_Repebody - 6xHis - GGS3 - T25 The T25 fragment of adenylate cyclase fused to the ssDNA-optimized-mCherry-binding Repebody using a GGS3 - linker for increased flexibility. When the Repebody binds mCherry fused to the other fragment, the two subunits start a mechanism that indicates if the BACTH system is working. The His-Tag is for experimental validation.
BBa_25QRQ2FK ssDNA-optimized-mCherry-binding_Repebody - 6xHis - T25 The T25 fragment of adenylate cyclase fused to the ssDNA-optimized-mCherry-binding Repebody. When the Repebody binds mCherry fused to the other fragment, the two subunits start a mechanism that indicates if the BACTH system is working. The His-Tag is for experimental validation.
BBa_25FKOX5Q T25 - GGS3 - mCherry-binding_Repebody - 6xHis The T25 fragment of adenylate cyclase fused to the mCherry-binding Repebody using a GGS3 - linker for increased flexibility. When the Repebody binds mCherry fused to the other fragment, the two subunits start a mechanism that indicates if the BACTH system is working. The His-Tag is for experimental validation.
BBa_2540IZIE T25 - mCherry-binding_Repebody - 6xHis The T25 fragment of adenylate cyclase fused to the mCherry-binding Repebody. When the Repebody binds mCherry fused to the other fragment, the two subunits start a mechanism that indicates if the BACTH system is working. The His-Tag is for experimental validation.
BBa_252LEBS1 T25 - GGS3 - ssDNA-optimized-mCherry-binding_Repebody - 6xHis The T25 fragment of adenylate cyclase fused to the ssDNA-optimized-mCherry-binding Repebody using a GGS3 - linker for increased flexibility. When the Repebody binds mCherry fused to the other fragment, the two subunits start a mechanism that indicates if the BACTH system is working. The His-Tag is for experimental validation.
BBa_25HK2H4O T25 - ssDNA-optimized-mCherry-binding_Repebody - 6xHis The T25 fragment of adenylate cyclase fused to the ssDNA-optimized-mCherry-binding Repebody. When the Repebody binds mCherry fused to the other fragment, the two subunits start a mechanism that indicates if the BACTH system is working. The His-Tag is for experimental validation.
BBa_25EO6BEC mCherry - HA - GGS3 - T18 The T18 fragment of adenylate cyclase fused to mCherry using a GGS3 - linker for increased flexibility. When the mCherry is bound by the Repebody fused to the other fragment, the two subunits start a mechanism that indicates if the BACTH system is working. The HA-Tag is for experimental validation.
BBa_25TAHASE mCherry - HA - T18 The T18 fragment of adenylate cyclase fused to mCherry. When the mCherry is bound by the Repebody fused to the other fragment, the two subunits start a mechanism that indicates if the BACTH system is working. The HA-Tag is for experimental validation.
BBa_259SSWC8 EGFP - HA - GGS3 - T18 The T18 fragment of adenylate cyclase fused to EGFP using a GGS3 - linker for increased flexibility. The EGFP should not be bound by the Repebody fused to the other fragment, so the two subunits aren’t able to interact and start the indicating mechanism. Therefore the part acts as a negative control for the BACTH system. The HA-Tag is for experimental validation.
BBa_253I2YTC EGFP - HA - T18 The T18 fragment of adenylate cyclase fused to EGFP. The EGFP should not be bound by the Repebody fused to the other fragment, so the two subunits aren’t able to interact and start the indicating mechanism. Therefore the part acts as a negative control for the BACTH system. The HA-Tag is for experimental validation.
BBa_2584YQ8N T18 - GGS3 - mCherry - HA The T18 fragment of adenylate cyclase fused to mCherry using a GGS3 - linker for increased flexibility. When the mCherry is bound by the Repebody fused to the other fragment, the two subunits start a mechanism that indicates if the BACTH system is working. The HA-Tag is for experimental validation.
BBa_25TTPMRP T18 - mCherry - HA The T18 fragment of adenylate cyclase fused to mCherry. When the mCherry is bound by the Repebody fused to the other fragment, the two subunits start a mechanism that indicates if the BACTH system is working. The HA-Tag is for experimental validation.
BBa_25V2YRYF T18 - GGS3 - EGFP - HA The T18 fragment of adenylate cyclase fused to EGFP using a GGS3 - linker for increased flexibility. The EGFP should not be bound by the Repebody fused to the other fragment, so the two subunits aren’t able to interact and start the indicating mechanism. Therefore the part acts as a negative control for the BACTH system. The HA-Tag is for experimental validation.
BBa_25H5OOE1 T18 - EGFP - HA The T18 fragment of adenylate cyclase fused to EGFP. The EGFP should not be bound by the Repebody fused to the other fragment, so the two subunits aren’t able to interact and start the indicating mechanism. Therefore the part acts as a negative control for the BACTH system. The HA-Tag is for experimental validation.
BBa_259CFCD5 EGFP-mCh A complex consisting out of fluorophores mCherry and eGFP
BBa_257H72S6 mCh-EGFP-Fis1 A complex that localizes the fluorophores mCherry and eGFP at the outer membrane of mitochondria through FIS1.
BBa_25AWGE3A mGBP-4GGS-mTagBFP2-4GGS-HA-A1E Negative control for lysosomal degradation of EGFP-labeled target
BBa_25SH19I1 GBP-4GGS-mTagBFP2-4GGS-HA-A1E Targets EGFP for lysosomal degradation
BBa_252J7V7J Plasmid internal targeting Retron with RiboJ Repebody editing retron with rIboj
BBa_25ZTWID8 EGFP post sdm EGFP used in EGFP-mCherry target
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