| BBa_25T0IA49 |
EGFP - HA-tag |
Encodes for enhanced green fluorescent protein (eGFP). C-terminal HA-tag added for analysis. |
| BBa_25GR2JDM |
mCherry - HA-tag |
Encodes for red fluorescent protein (mCherry). C-terminal HA-tag added for analysis. |
| BBa_253J3LF3 |
mCherry-binding Repebody-4GGS-mTagBFP2-4GGS-HA-A1E |
Repebody degrader |
| BBa_2558RZ0V |
mCherry-binding Repebody-4GGS-mTagBFP2-HA |
Non-binding negative control for the Repebody-degrader |
| BBa_25WLKZDM |
EGFP[NdeI-]-HATag-’tgataa' |
HATagged EGFP for pCDFDuet MCS activity testing |
| BBa_25F2CTKF |
mCherry[NcoI-]-HisTag-’tgataa' |
HisTagged mCherry for pCDFDuet MCS activity testing |
| BBa_25A9293O |
Repebody[mutopt]-HisTag-’tgataa' |
His tagged mutation-optimized Repebody for pCDFDuetIso expression |
| BBa_25H9D35L |
mCherry-binding_Repebody - 6xHis - GGS3 - T25 |
The T25 fragment of adenylate cyclase fused to the mCherry-binding Repebody using a GGS3 - linker for increased flexibility. When the Repebody binds mCherry fused to the other fragment, the two subunits start a mechanism that indicates if the BACTH system is working. The His-Tag is for experimental validation. |
| BBa_255QHYDE |
mCherry-binding_Repebody - 6xHis - T25 |
The T25 fragment of adenylate cyclase fused to the mCherry-binding Repebody. When the Repebody binds mCherry bound to the other fragment, the two subunits start a mechanism that indicates if the BACTH system is working. The His-Tag is for experimental validation. |
| BBa_25L4RPGJ |
ssDNA-optimized-mCherry-binding_Repebody - 6xHis - GGS3 - T25 |
The T25 fragment of adenylate cyclase fused to the ssDNA-optimized-mCherry-binding Repebody using a GGS3 - linker for increased flexibility. When the Repebody binds mCherry fused to the other fragment, the two subunits start a mechanism that indicates if the BACTH system is working. The His-Tag is for experimental validation. |
| BBa_25QRQ2FK |
ssDNA-optimized-mCherry-binding_Repebody - 6xHis - T25 |
The T25 fragment of adenylate cyclase fused to the ssDNA-optimized-mCherry-binding Repebody. When the Repebody binds mCherry fused to the other fragment, the two subunits start a mechanism that indicates if the BACTH system is working. The His-Tag is for experimental validation. |
| BBa_25FKOX5Q |
T25 - GGS3 - mCherry-binding_Repebody - 6xHis |
The T25 fragment of adenylate cyclase fused to the mCherry-binding Repebody using a GGS3 - linker for increased flexibility. When the Repebody binds mCherry fused to the other fragment, the two subunits start a mechanism that indicates if the BACTH system is working. The His-Tag is for experimental validation. |
| BBa_2540IZIE |
T25 - mCherry-binding_Repebody - 6xHis |
The T25 fragment of adenylate cyclase fused to the mCherry-binding Repebody. When the Repebody binds mCherry fused to the other fragment, the two subunits start a mechanism that indicates if the BACTH system is working. The His-Tag is for experimental validation. |
| BBa_252LEBS1 |
T25 - GGS3 - ssDNA-optimized-mCherry-binding_Repebody - 6xHis |
The T25 fragment of adenylate cyclase fused to the ssDNA-optimized-mCherry-binding Repebody using a GGS3 - linker for increased flexibility. When the Repebody binds mCherry fused to the other fragment, the two subunits start a mechanism that indicates if the BACTH system is working. The His-Tag is for experimental validation. |
| BBa_25HK2H4O |
T25 - ssDNA-optimized-mCherry-binding_Repebody - 6xHis |
The T25 fragment of adenylate cyclase fused to the ssDNA-optimized-mCherry-binding Repebody. When the Repebody binds mCherry fused to the other fragment, the two subunits start a mechanism that indicates if the BACTH system is working. The His-Tag is for experimental validation. |
| BBa_25EO6BEC |
mCherry - HA - GGS3 - T18 |
The T18 fragment of adenylate cyclase fused to mCherry using a GGS3 - linker for increased flexibility. When the mCherry is bound by the Repebody fused to the other fragment, the two subunits start a mechanism that indicates if the BACTH system is working. The HA-Tag is for experimental validation. |
| BBa_25TAHASE |
mCherry - HA - T18 |
The T18 fragment of adenylate cyclase fused to mCherry. When the mCherry is bound by the Repebody fused to the other fragment, the two subunits start a mechanism that indicates if the BACTH system is working. The HA-Tag is for experimental validation. |
| BBa_259SSWC8 |
EGFP - HA - GGS3 - T18 |
The T18 fragment of adenylate cyclase fused to EGFP using a GGS3 - linker for increased flexibility. The EGFP should not be bound by the Repebody fused to the other fragment, so the two subunits aren’t able to interact and start the indicating mechanism. Therefore the part acts as a negative control for the BACTH system. The HA-Tag is for experimental validation. |
| BBa_253I2YTC |
EGFP - HA - T18 |
The T18 fragment of adenylate cyclase fused to EGFP. The EGFP should not be bound by the Repebody fused to the other fragment, so the two subunits aren’t able to interact and start the indicating mechanism. Therefore the part acts as a negative control for the BACTH system. The HA-Tag is for experimental validation. |
| BBa_2584YQ8N |
T18 - GGS3 - mCherry - HA |
The T18 fragment of adenylate cyclase fused to mCherry using a GGS3 - linker for increased flexibility. When the mCherry is bound by the Repebody fused to the other fragment, the two subunits start a mechanism that indicates if the BACTH system is working. The HA-Tag is for experimental validation. |
| BBa_25TTPMRP |
T18 - mCherry - HA |
The T18 fragment of adenylate cyclase fused to mCherry. When the mCherry is bound by the Repebody fused to the other fragment, the two subunits start a mechanism that indicates if the BACTH system is working. The HA-Tag is for experimental validation. |
| BBa_25V2YRYF |
T18 - GGS3 - EGFP - HA |
The T18 fragment of adenylate cyclase fused to EGFP using a GGS3 - linker for increased flexibility. The EGFP should not be bound by the Repebody fused to the other fragment, so the two subunits aren’t able to interact and start the indicating mechanism. Therefore the part acts as a negative control for the BACTH system. The HA-Tag is for experimental validation. |
| BBa_25H5OOE1 |
T18 - EGFP - HA |
The T18 fragment of adenylate cyclase fused to EGFP. The EGFP should not be bound by the Repebody fused to the other fragment, so the two subunits aren’t able to interact and start the indicating mechanism. Therefore the part acts as a negative control for the BACTH system. The HA-Tag is for experimental validation. |
| BBa_259CFCD5 |
EGFP-mCh |
A complex consisting out of fluorophores mCherry and eGFP |
| BBa_257H72S6 |
mCh-EGFP-Fis1 |
A complex that localizes the fluorophores mCherry and eGFP at the outer membrane of mitochondria through FIS1. |
| BBa_25AWGE3A |
mGBP-4GGS-mTagBFP2-4GGS-HA-A1E |
Negative control for lysosomal degradation of EGFP-labeled target |
| BBa_25SH19I1 |
GBP-4GGS-mTagBFP2-4GGS-HA-A1E |
Targets EGFP for lysosomal degradation |
| BBa_252J7V7J |
Plasmid internal targeting Retron with RiboJ |
Repebody editing retron with rIboj |
| BBa_25ZTWID8 |
EGFP post sdm |
EGFP used in EGFP-mCherry target |