All of our level 0 plasmids (chloramphenicol resistance) were verified by Sanger sequencing (we attached electropherogram raw data as ab1 file with those parts), using one of the primers below.
U174_Kan-F 5-attctcaccggattcagt-3 U158_T7 5-taatacgactcactatagg-3 End-of-CmR 5-gcttaatgaattacaacagtactgcg-3 BR322ori-F 5-gggaaacgcctggtatcttt-3 t0terminator_R 5-ttcagaacgctcggttgcc-3
We made the following level 0 parts
| Level 0 Part | SBOL Type | Lee Type | Part Name | Sequencing Verified |
|---|---|---|---|---|
| BBa_25MIG4EW | Homologous Region | Type 8b | HR5'_ACE2_Chr12L | DOI: 10.5281/zenodo.17274640 |
| BBa_259HCU8C | Homologous Region | Type 7 | HR3'_ACE2_Chr12R | DOI: 10.5281/zenodo.17274769 |
| BBa_256S6J1M | coding | Type 3 | HsDOR | DOI: 10.5281/zenodo.17274754 |
| BBa_25RCU5CB | coding | Type 3 | DrDHCR7 | DOI: 10.5281/zenodo.17274657 |
| BBa_25FOVO4C | coding | Type 3 | DrDHCR24 | DOI: 10.5281/zenodo.17274626 |
| BBa_K5441013 | coding | Type 3 | BAX | DOI: 10.5281/zenodo.17276880 |
| BBa_250R9OVR | coding | Type 3 | IME1 | DOI: 10.5281/zenodo.17274737 |
| BBa_K5470009 | coding | GSG-E2A | DOI: 10.5281/zenodo.17272256 | |
| BBa_25FQBGJW | promoter | Type 2 | pSTM1 | DOI: 10.5281/zenodo.17276264 |
| BBa_259JX52V | promoter | Type 2 | pOST1 | DOI: 10.5281/zenodo.17274792 |
| BBa_K3748013 | promoter | Type 2 | pRNR2 | DOI: 10.5281/zenodo.17276585 |
| BBa_25AIDL8P | 3'UTR | Type 3b | ASH1 3'UTR | DOI: 10.5281/zenodo.17274570 |
| BBa_25TQG9WZ | coding | Type 3a | modified mCherry | DOI: 10.5281/zenodo.17274695 |
| BBa_25VHXKNL | promoter | Type 2 | ASH1 AIpro | DOI: 10.5281/zenodo.17274719 |
We gratefully acknowledge the following companies for synthesizing double-strand DNA fragments used in our project: Tsingke, GenScript, Twist Bioscience, and Integrated DNA Technologies. All of our Golden Gate assembled plasmids, were verified by restriction enzyme digestion (NotI or HindIII) and whole-plasmid sequencing.
Our favourite part is BBa_255T0PHY which belongs to the TU Recorders collection.
Please visit our Improved Parts page for parts we improved this year.
Vist our Part Collection page for details about our 3 collections.
Lee, M. E., DeLoache, W. C., Cervantes, B., & Dueber, J. E. (2015). A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. ACS synthetic biology, 4(9), 975–986. DOI: 10.1021/sb500366v
