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Background

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Plant bacterial wilt, caused by the soil-borne bacterium Ralstonia, infects over 400 crops including tomatoes and peppers.

In southern China (such as Guangdong), field incidence can reach 50–80%, causing severe annual losses.

However...

Current Limitations

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Traditional diagnosis depends on visual symptoms or laboratory tests. Visual checks find the disease too late, while lab tests are costly and not field-friendly.

Our Solution

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Ralstonia releases a special signaling molecule (AHL) early in infection. Our engineered E. coli detects it and triggers a warning signal.

How It Works - Step 1

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Insert a sampler into the soil, filter the soil, collect AHL molecules in the soil, and transfer them to a vacuum collection tube.

How It Works - Step 2

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Transfer the liquid containing engineered bacteria into a vacuum tube, then incubate it in a shaking incubator for batch testing.When the result turns blue, it indicates that the sample is infected with bacterial wilt.

Advantages

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This synthetic biology approach is portable, low-cost, and effective in the field.

Future Outlook

It gives farmers critical time to protect their crops and reduce losses.

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