Revolutionizing cancer relapse detection through innovative synthetic biology and advanced biosensing
We are contributing a small library of tetracycline-mimic inhibitor peptide (TIP) expression cassettes that implement threshold control in Tet-regulated circuits. TIP binds to tTA and prevents its binding to tetO, raising the activation threshold of Tet-responsive reporters. All three plasmids carry an N-terminal 3×FLAG tag for easy detection and are intended for use alongside SynNotch→Tet circuits (or any Tet-Off module).
A 3×FLAG-TIP sequence which binds to the TetR domain of tTA and prevents it from binding to DNA tetO domains. This fundamental inhibitor component forms the basis of our thresholding system, providing precise control over transcriptional activation.
For thresholding to be predictable, the inhibitor must be expressed consistently. FLAG-TIP ensures reliable, reproducible control over tTA activity, making it the cornerstone of dose-response characterization and quantitative analysis in synthetic biology circuits.
N-terminal 3×FLAG-TIP fusion protein.
TIP directly binds to the TetR domain of tTA, preventing it from binding to DNA tetO domains.
Designed for consistent expression to ensure predictable thresholding behavior.
Primary choice for dose-response mapping and experiments where low variance is critical.
Strong suppression with the tightest confidence intervals among promoters tested.
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