Overview

Throughout the many stages of our project, we ensured that our work evolved in response to feedback from our stakeholders and various Human Practices activities. We studied how our project impacted the world and how the world, in turn, impacted our project. This approach allowed us to validate the rationale behind our project while also allowing us to implement solutions that address the needs and concerns of our community. From educating ourselves by learning from our stakeholders to educating our community using the insights we gained, we established feedback loops that guided our project from start to finish. This iterative process allowed LANCET to improve in all aspects and become the most effective version of itself.

Stakeholder Interactions

For each stakeholder interaction we had, we recorded details in a spreadsheet, including the date, team member participants, and comprehensive notes on key insights. This allowed us to maintain a clear and organized record of our engagement process for future analysis and reflection (see Fig. 1).

Fig. 1. Lambert iGEM 2025 Contact Log

Next, we categorized stakeholders according to their relevance to different aspects of our project. This helped us understand their impact and priorities while also ensuring we considered a wide range of perspectives to incorporate a variety of stakeholder views (see Fig. 2).

Fig. 2. LANCET Stakeholder Categorization

T.I.C.K. Analysis

Finally, we analyzed each stakeholder interaction using a structured, four-step process called T.I.C.K.:Target, Investigate, Consider, and Kickstart. This process ensured that feedback was not only accurately documented but also meaningfully applied to our project (see Fig. 3).

Fig. 3. T.I.C.K. Analysis Process

Mr Nate LynamMrs. Joanna Spalding | Educators & TeachersDr. Imre Banlaki #1 | Cell-Free System ExpertsDr. Imre Banlaki #2 | Cell-Free System ExpertsMr. Marten Meckelburg #1 | Cell-Free System ExpertsMr. Marten Meckelburg #2 | Cell-Free System ExpertsMr. Marten Meckelburg #3 | Cell-Free System ExpertsMr. Marten Meckelburg #4 | Cell-Free System ExpertsDr. Thomas H. Murray | Bioethics & Policy MakersDr. Mark Styczynski #1 | Modeling & Computational ExpertsDr. Mark Styczynski #2 | Modeling & Computational ExpertsDr. Mark Styczynski #3 | Modeling & Computational ExpertsDr. Feng-Ming Wang | Modeling & Computational ExpertsDr. Daniel Richards | Modeling & Computational ExpertsThe Food and Drug Administration (FDA) | Health & Regulatory AgenciesDr. Thomas Hart | Scientific ResearcherDr. Nicole Baumgarth | Scientific ResearcherProfessor George Chaconas | Scientific ResearcherMrs. Catherine O’Haver #1 | Healthcare Practitioners & Clinical AdvisorsMrs. Catherine O’Haver #2 | Healthcare Practitioners & Clinical AdvisorsMrs. Zoe Demko | Healthcare Practitioners & Clinical AdvisorsDr. Mike McLaughlin | Healthcare Practitioners & Clinical AdvisorsMs. Kendall Lundberg | Lyme VoicesAnonymous No. 1 | Lyme VoicesAnonymous No. 2 | Lyme VoicesMs. Beth S. | Lyme VoicesSignalChem Diagnostics #1 | Biotech & Diagnostic DevelopersSignalChem Diagnostics #2 | Biotech & Diagnostic DevelopersSignalChem Diagnostics #3 | Biotech & Diagnostic DevelopersMr. Anthony Shin | Biotech & Diagnostic DevelopersMs. Allyson Read | Inclusivity & Community Representation

Reevaluations

As our project came to a close, we reconnected with our stakeholders to ask for their thoughts on our completed project by sharing a video presentation. After reviewing our wet lab results, frugal hardware, and diverse human practices approaches, they expressed strong support—highlighting how the project grew over time and integrated scientific progress with community impact. Below are some of the many comments we received:

• Dr. Nicole Baumgarth

  Congratulations on a job very well done indeed! This must have been so much work, and you did really well. Very, very impressive - and thank you for sharing and seeking feedback.

• Professor George Chaconas

  Thank you for sharing your video, which I just watched. It’s very impressive and you have done a great job. The quality and creativity of your work is very evident to me. Keep up the great work!

• Mrs. Catherine O’Haver

  I’m so proud of all of you for the work on this project. The video is excellent.

• Dr. Imre Banlaki

  Thanks for the video, the lateral flow diagnostic looks very cool! I’m impressed the isothermal amplification worked so well – it’s usually a pain.

Conclusion

By gathering insights from experts to improve our project, we ensured that our stakeholders’ feedback was integrated throughout every stage of LANCET. Carefully documenting and reflecting on each interaction allowed us to actively learn from diverse stakeholders, strengthening our project while also identifying areas for improvement and refining our approach. We continuously adapted and updated different aspects of LANCET in response to the feedback we received, ensuring that the project evolved to meet real-world needs. With stakeholder engagement at the heart of everything we did, LANCET was shaped into a responsible project that provides a positive impact on the world around us.