Intro
Sialic acid plays a vital role in regulating physiological and biochemical functions in the human body,
exhibiting multiple biological activities including anti-inflammatory properties, influenza treatment, etc.
We aim to develop an efficient production method for sialic acid in order to solve several limitations current production methods have.
Solution
In order to produce sialic acid, we designed three different methods
with expressions of different target genes: AGE-NAL, AGE-NAL with Scaffolding, AGE-Linker-NAL, and our results turned out
to be our results turned out to be successful for all these methods
qualitatively, but with various effeciencies quantitatively.
Mechanism
Among all different methods, the most effective one is AGE-NAL with Scaffolding as target gene. The Scaffolding protein connects AGE and NAL and produce sialic acid from the substrate in a high yeild.
We used two types of E. coli for the transformation of our reconstructed plasmids: DH5α for sequence verification and BL21 for protein expression and functional testing.
Our project will leverage multi-enzyme combination whole-cell catalysis technology to explore scalable biological production methods for sialic acid. This will facilitate the efficient preparation and widespread application of novel protein-based products.
