LOCUS FG-pESC-HIS 8994 bp DNA circular SYN 26-SEP-2025 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 8994) AUTHORS . TITLE Direct Submission JOURNAL Exported Oct 4, 2025 from SnapGene 8.1.1 https://www.snapgene.com FEATURES Location/Qualifiers source 1..8994 /mol_type="other DNA" /organism="recombinant plasmid" promoter 317..503 /gene="S. cerevisiae HIS3" /label=HIS3 promoter CDS 504..1163 /codon_start=1 /gene="S. cerevisiae HIS3" /product="imidazoleglycerol-phosphate dehydratase, required for histidine biosynthesis" /label=HIS3 /note="yeast auxotrophic marker" /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF KEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFL ESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM" rep_origin complement(1423..1878) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" terminator 1961..2126 /gene="S. cerevisiae ADH1" /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" primer_bind 1961..1984 /label=tADH1-R primer_bind complement(2239..2267) /label=FGVec-R primer_bind 2248..2292 /label=FGIns-F misc_feature 2256..2267 /label=MCS 1 /note="multiple cloning site 1" CDS complement(2271..2978) /label=mCherry primer_bind complement(2595..2619) /label=mCherrF primer_bind complement(2957..2988) /label=片段 1.正向 primer_bind 2969..2993 /label=片段 2.反向 misc_feature 2979..3012 /label=psiMCS /note="multiple cloning site 1" promoter complement(3017..4652) /label=pFUS1 primer_bind complement(4625..4662) /label=片段 2.正向 primer_bind 4643..4686 /label=片段 3.正向 promoter 4653..5296 /gene="S. cerevisiae TDH3" /label=GAP promoter /note="promoter for glyceraldehyde-3-phosphate dehydrogenase; also known as the TDH3 promoter" primer_bind 4653..4685 /label=pGAP-F primer_bind complement(5253..5316) /label=FGIns-R primer_bind 5297..5319 /label=FGVec-F misc_feature 5300..5376 /label=MCS 2 /note="multiple cloning site 2" CDS 5343..5372 /codon_start=1 /product="Myc (human c-Myc oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" terminator 5402..5591 /gene="S. cerevisiae CYC1" /label=CYC1 terminator /note="transcription terminator for CYC1" primer_bind complement(5520..5538) /label=tCYC1-R rep_origin complement(5834..6422) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(join(6593..7384,7385..7453)) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(7454..7558) /gene="bla" /label=AmpR promoter rep_origin 7585..8927 /label=2u ori /note="yeast 2u plasmid origin of replication" BASE COUNT 2410 a 2011 c 2033 g 2540 t ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accataaatt cccgttttaa gagcttggtg agcgctagga gtcactgcca ggtatcgttt 241 gaacacggca ttagtcaggg aagtcataac acagtccttt cccgcaattt tctttttcta 301 ttactcttgg cctcctctag tacactctat atttttttat gcctcggtaa tgattttcat 361 tttttttttt cccctagcgg atgactcttt ttttttctta gcgattggca ttatcacata 421 atgaattata cattatataa agtaatgtga tttcttcgaa gaatatacta aaaaatgagc 481 aggcaagata aacgaaggca aagatgacag agcagaaagc cctagtaaag cgtattacaa 541 atgaaaccaa gattcagatt gcgatctctt taaagggtgg tcccctagcg atagagcact 601 cgatcttccc agaaaaagag gcagaagcag tagcagaaca ggccacacaa tcgcaagtga 661 ttaacgtcca cacaggtata gggtttctgg accatatgat acatgctctg gccaagcatt 721 ccggctggtc gctaatcgtt gagtgcattg gtgacttaca catagacgac catcacacca 781 ctgaagactg cgggattgct ctcggtcaag cttttaaaga ggccctactg gcgcgtggag 841 taaaaaggtt tggatcagga tttgcgcctt tggatgaggc actttccaga gcggtggtag 901 atctttcgaa caggccgtac gcagttgtcg aacttggttt gcaaagggag aaagtaggag 961 atctctcttg cgagatgatc ccgcattttc ttgaaagctt tgcagaggct agcagaatta 1021 ccctccacgt tgattgtctg cgaggcaaga atgatcatca ccgtagtgag agtgcgttca 1081 aggctcttgc ggttgccata agagaagcca cctcgcccaa tggtaccaac gatgttccct 1141 ccaccaaagg tgttcttatg tagtgacacc gattatttaa agctgcagca tacgatatat 1201 atacatgtgt atatatgtat acctatgaat gtcagtaagt atgtatacga acagtatgat 1261 actgaagatg acaaggtaat gcatcattct atacgtgtca ttctgaacga ggcgcgcttt 1321 ccttttttct ttttgctttt tctttttttt tctcttgaac tcgacggatc tatgcggtgt 1381 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata 1441 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg 1501 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc 1561 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa 1621 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt 1681 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac 1741 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta 1801 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg 1861 cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc 1921 gatcggtgcg ggcctcttcg ctattacgcc agctgaattg gagcgacctc atgctatacc 1981 tgagaaagca acctgaccta caggaaagag ttactcaaga ataagaattt tcgttttaaa 2041 acctaagagt cactttaaaa tttgtataca cttatttttt ttataactta tttaataata 2101 aaaatcataa atcataagaa attcgcttat ttagaagtgt caacaacgta tctaccaacg 2161 atttgaccct tttccatctt ttcgtaaatt tctggcaagg tagacaagcc gacaaccttg 2221 attggagact tgaccaaacc tctggcgaag aattgttaat taagagctta cttgtacagc 2281 tcatccatgc cgcccgtgct atgacgtcct tcggcacgct cgtactgctc cacgatggtg 2341 tagtcctcgt tgtggctggt gatgtccagc ttgatgttca cgttgtaggc gccgggcaat 2401 tgcacgggct tcttggcctt gtaggtggtc ttcacctcgg cgtcgtagtg gcctccatcc 2461 ttcagcttca ggcgctgctt gatctcgccc ttcagagcgc cgtcctcggg atacatgcgc 2521 tcgctggaag cctcccagcc catggtcttc ttctgcatca ccgggccatc ggagggaaag 2581 ttcgtgccgc gcagcttgac cttgtagatg aactcgccgt cctgcaagct ggagtcctgg 2641 gtcacggtca ccactccgcc gtcctcgaag ttcatcacgc gctcccactt gaagccttcg 2701 gggaagctca gcttcaggta gtcgggaatg tcggccgggt gcttcacgta ggccttggag 2761 ccgtacatga actgggggct caagatgtcc caggcaaagg gcaacgggcc tcccttggtc 2821 accttcagtt tggcggtctg ggtgccttcg tagggacgtc cctcgccttc gccctcgatc 2881 tcgaactcgt ggccattcac gctgccctcc atgtggacct tgaagcgcat gaactccttg 2941 atgatggcca tgttatcctc ttctcccttg gacaccatct agtgcggccg ccctttagtg 3001 agggttgaat tcgaattttg attttcagaa acttgatggc ttatatcctg ctcttaaagg 3061 aaaaaagata aaggatgtcg tatccaaata ttttaagagc aacacatacc ttacaaagcc 3121 actcttacat tgtcatttaa ataaaaccaa tcccgagaat tacgttattt gtttcaaatt 3181 acagacgttt catgtttgtt tcatcatcgc accaaaatga gacagcgcga aaagtgacag 3241 tgcaattatg ccattcaata cgtcaaaaag ggcatcctgc agagatacaa ctctgttttg 3301 aaagggtcca catggtaaat atggaagcaa aagaaggatt gaggcatcac tgacaacaaa 3361 attgtcttgc agcccaatta ataaaaaaaa ataaagttat atatataaac aaatacgtaa 3421 acaaaggata gaaggcgaac tgaatcatcg ttcagtagcg gttctggttg ccgttattgt 3481 tctgttgaga aaagttgcca gaaaaattga aggattcgtg ctgtccattg gagttctggt 3541 ttccgccgta ttgcggtctt ccgtactcat tggattgctg ttgaccattc tgttgcggtc 3601 taccatactc attagcctgt tgctggcccc cataactcga atttgaattg gagttattgc 3661 ccaggtagga acttgccatg gaagccaaag ctgagaatga actggagtgg ccttgttgct 3721 gctgctgacc ctgttgttga tgctgatagt tttggccttg ttgctggtat tgctgctggt 3781 tgttttggcc ttgttgattg tattgctgtt ggttggattg agtttgtcca ccacctaagt 3841 aagattgagc catggaggcc aatgaggaaa aagcaccacc agattgtccc tgttgttgtt 3901 ggtaaccacc gttgccacct tggtactgtt gattattgta accttgacta ttttggttac 3961 cgttttgata ggattggtta tagccctgtt gactattgtt ggagttctga ttattattgg 4021 aatgcataaa agagcttgcc atagaagcca gtgctccaaa ggaggaacca ccggagcttt 4081 gattttgacc ttgctgatta ttgttgttgg aattcatgaa agatgaagcc aaagacgcca 4141 aagcagtaaa agaaccttga ccttgacctt gtccttgacc ttgaccttga ccttgacctt 4201 gtccctgacc ttgggaattg ttacctgact taaagaattg agaagccaag gcagccaggc 4261 cggaagcacc catactttgg ctcccgccgc cagaaccact tagcattgac atgacagtcc 4321 ctacgtcaaa ccctctgtta gaagaacctt tatttgatga gtgtgtcatc actgtggcta 4381 acaaagctag ttttcctagt tgcgtttggg aagaaccctt agagtctgcc attaaagtgt 4441 tcataaaaga tgaaccacca ccagcagcac gatcttgcga ggcgtcacta ccaccactac 4501 ggttgtccat gacgtatcct gcgatttttt gaattaatga ttcaatagtt gacatttgct 4561 cgtcattggg gttcgactga gctgcggatg tcaacttcgc aacagcttct gcatggtttc 4621 cttgagaaaa atgagactca gcctctgaga tttcattatc aatactcgcc atttcaaaga 4681 atacgtaaat aattaatagt agtgattttc ctaactttat ttagtcaaaa aattagcctt 4741 ttaattctgc tgtaacccgt acatgcccaa aatagggggc gggttacaca gaatatataa 4801 catcgtaggt gtctgggtga acagtttatt cctggcatcc actaaatata atggagcccg 4861 ctttttaagc tggcatccag aaaaaaaaag aatcccagca ccaaaatatt gttttcttca 4921 ccaaccatca gttcataggt ccattctctt agcgcaacta cagagaacag gggcacaaac 4981 aggcaaaaaa cgggcacaac ctcaatggag tgatgcaacc tgcctggagt aaatgatgac 5041 acaaggcaat tgacccacgc atgtatctat ctcattttct tacaccttct attaccttct 5101 gctctctctg atttggaaaa agctgaaaaa aaaggttgaa accagttccc tgaaattatt 5161 cccctacttg actaataagt atataaagac ggtaggtatt gattgtaatt ctgtaaatct 5221 atttcttaaa cttcttaaat tctactttta tagttagtct tttttttagt tttaaaacac 5281 cagaacttag tttcgacccg gatccgtaat acgactcact atagggcccg ggcgtcgaca 5341 tggaacagaa gttgatttcc gaagaagacc tcgagtaagc ttggtaccgc ggctagctaa 5401 gatccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc cctatttatt 5461 tttttatagt tatgttagta ttaagaacgt tatttatatt tcaaattttt cttttttttc 5521 tgtacagacg cgtgtacgca tgtaacatta tactgaaaac cttgcttgag aaggttttgg 5581 gacgctcgaa gatccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 5641 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 5701 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 5761 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 5821 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 5881 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 5941 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 6001 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 6061 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 6121 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 6181 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 6241 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc 6301 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 6361 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 6421 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 6481 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 6541 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 6601 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 6661 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 6721 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 6781 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 6841 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 6901 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 6961 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 7021 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 7081 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 7141 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 7201 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 7261 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 7321 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 7381 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 7441 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 7501 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 7561 catttccccg aaaagtgcca cctgaacgaa gcatctgtgc ttcattttgt agaacaaaaa 7621 tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag 7681 aaatgcaacg cgaaagcgct attttaccaa cgaagaatct gtgcttcatt tttgtaaaac 7741 aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag 7801 aacagaaatg caacgcgaga gcgctatttt accaacaaag aatctatact tcttttttgt 7861 tctacaaaaa tgcatcccga gagcgctatt tttctaacaa agcatcttag attacttttt 7921 ttctcctttg tgcgctctat aatgcagtct cttgataact ttttgcactg taggtccgtt 7981 aaggttagaa gaaggctact ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc 8041 acttcccgcg tttactgatt actagcgaag ctgcgggtgc attttttcaa gataaaggca 8101 tccccgatta tattctatac cgatgtggat tgcgcatact ttgtgaacag aaagtgatag 8161 cgttgatgat tcttcattgg tcagaaaatt atgaacggtt tcttctattt tgtctctata 8221 tactacgtat aggaaatgtt tacattttcg tattgttttc gattcactct atgaatagtt 8281 cttactacaa tttttttgtc taaagagtaa tactagagat aaacataaaa aatgtagagg 8341 tcgagtttag atgcaagttc aaggagcgaa aggtggatgg gtaggttata tagggatata 8401 gcacagagat atatagcaaa gagatacttt tgagcaatgt ttgtggaagc ggtattcgca 8461 atattttagt agctcgttac agtccggtgc gtttttggtt ttttgaaagt gcgtcttcag 8521 agcgcttttg gttttcaaaa gcgctctgaa gttcctatac tttctagaga ataggaactt 8581 cggaatagga acttcaaagc gtttccgaaa acgagcgctt ccgaaaatgc aacgcgagct 8641 gcgcacatac agctcactgt tcacgtcgca cctatatctg cgtgttgcct gtatatatat 8701 atacatgaga agaacggcat agtgcgtgtt tatgcttaaa tgcgtactta tatgcgtcta 8761 tttatgtagg atgaaaggta gtctagtacc tcctgtgata ttatcccatt ccatgcgggg 8821 tatcgtatgc ttccttcagc actacccttt agctgttcta tatgctgcca ctcctcaatt 8881 ggattagtct catccttcaa tgctatcatt tcctttgata ttggatcatc taagaaacca 8941 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc //