LOCUS pESC-URA-pOpM-C1ND-Sed1 8097 bp DNA circular SYN 06-OCT-2025 DEFINITION OMs-C1ND-Sed1_pESC-URA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8097) AUTHORS GenScript TITLE Direct Submission JOURNAL Exported Oct 6, 2025 from SnapGene 8.1.1 https://www.snapgene.com COMMENT http://www.genscript.com/ ORIGDB|GenBank COMMENT Sequence Label: pESC-URA-pOpM-C1ND-Sed1 FEATURES Location/Qualifiers source 1..8097 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 196..416 /gene="S. cerevisiae URA3" /label=URA3 promoter /note="color: #ffffff; direction: RIGHT" promoter 201..416 /gene="S. cerevisiae URA3" /label=URA3 promoter /note="color: #ffffff" CDS 417..1220 /codon_start=1 /gene="S. cerevisiae URA3" /product="orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis" /label=URA3 /note="yeast auxotrophic marker, counterselectable with 5-fluoroorotic acid (5-FOA)" /note="color: #993366" /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN" rep_origin complement(1351..1806) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /note="color: #ffff00" terminator complement(1885..2050) /gene="S. cerevisiae ADH1" /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" /note="color: #ffffff; direction: LEFT" CDS complement(2198..2221) /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /note="color: #993366" /translation="DYKDDDDK" promoter 2268..2932 /gene="GAL1 and GAL10 genes of S. cerevisiae" /label=GAL1,10 promoter /note="divergent inducible promoter, regulated by Gal4" /note="color: #ffffff" protein_bind 2484..2601 /label=UAS /note="upstream activating sequence mediating Gal4-dependent induction" /note="color: #31849b" promoter 2943..2961 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" /note="color: #ffffff" sig_peptide 2969..3244 /codon_start=1 /label=preOst1-ProMF-alpha-1 /note="color: #ffff99" /translation="MRQVWFSWIVGLFLCFFNVSSAAPVNTTTEDETAQIPAEAVIGYS DLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKREAEA" CDS 3245..3262 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /note="color: #cc99b2" /translation="HHHHHH" CDS 3263..3583 /codon_start=1 /label=C1ND /note="color: #ccffcc" /translation="QLTTESMPFNVAEGKEVLLLVHNLPQQLFGYSWYKGERVDGNRQI VGYAIGTQQATPGPANSGRETIYPNASLLIQNVTQNDTGFYTLQVIKSDLVNEEATGQF HVY" CDS 3584..3628 /label=GSlinker /note="color: #999999; direction: RIGHT" CDS 3629..4468 /codon_start=1 /label=Sed1 /note="color: #993366" /translation="ALPTNGTSTEAPTDTTTEAPTTGLPTNGTTSAFPPTTSLPPSNTT TTPPYNPSTDYTTDYTVVTEYTTYCPEPTTFTTNGKTYTVTEPTTLTITDCPCTIEKPT TTSTTEYTVVTEYTTYCPEPTTFTTNGKTYTVTEPTTLTITDCPCTIEKPTTTSTTEYT VVTEYTTYCPEPTTFTTNGKTYTVTEPTTLTITDCPCTIEKSEAPESSVPVTESKGTTT KETGVTTKQTTANPSLTVSTVVPVSSSASSHSVVINSNGANVVVPGALGLAGVAMLFL" terminator 4504..4693 /gene="S. cerevisiae CYC1" /label=CYC1 terminator /note="transcription terminator for CYC1" /note="color: #ffffff" rep_origin complement(4936..5524) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" CDS complement(5695..6555) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /note="color: #993366" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(6556..6660) /gene="bla" /label=AmpR promoter /note="color: #ffffff; direction: LEFT" rep_origin complement(6687..8029) /direction=LEFT /label=2u ori /note="yeast 2u plasmid origin of replication" /note="color: #ffff00" protein_bind complement(7650..7697) /label=FRT /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." /note="color: #31849b; direction: LEFT" ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc 241 ggtttccttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg 301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc 361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt 421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat 481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca 541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg 601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg 661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca 721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac 781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa 841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg 901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct 961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac 1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg 1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa 1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa 1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac 1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac 1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat 1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa 1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca 1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg 1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta 1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg 1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa 1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg 1801 gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 1861 ttcgctatta cgccagctga attggagcga cctcatgcta tacctgagaa agcaacctga 1921 cctacaggaa agagttactc aagaataaga attttcgttt taaaacctaa gagtcacttt 1981 aaaatttgta tacacttatt ttttttataa cttatttaat aataaaaatc ataaatcata 2041 agaaattcgc ttatttagaa gtgtcaacaa cgtatctacc aacgatttga cccttttcca 2101 tcttttcgta aatttctggc aaggtagaca agccgacaac cttgattgga gacttgacca 2161 aacctctggc gaagaattgt taattaagag ctcagatctt atcgtcgtca tccttgtaat 2221 ccatcgatac tagtgcggcc gccctttagt gagggttgaa ttcgaatttt caaaaattct 2281 tacttttttt ttggatggac gcaaagaagt ttaataatca tattacatgg cattaccacc 2341 atatacatat ccatatacat atccatatct aatcttactt atatgttgtg gaaatgtaaa 2401 gagccccatt atcttagcct aaaaaaacct tctctttgga actttcagta atacgcttaa 2461 ctgctcattg ctatattgaa gtacggatta gaagccgccg agcgggtgac agccctccga 2521 aggaagactc tcctccgtgc gtcctcgtct tcaccggtcg cgttcctgaa acgcagatgt 2581 gcctcgcgcc gcactgctcc gaacaataaa gattctacaa tactagcttt tatggttatg 2641 aagaggaaaa attggcagta acctggcccc acaaaccttc aaatgaacga atcaaattaa 2701 caaccatagg atgataatgc gattagtttt ttagccttat ttctggggta attaatcagc 2761 gaagcgatga tttttgatct attaacagat atataaatgc aaaaactgca taaccacttt 2821 aactaatact ttcaacattt tcggtttgta ttacttctta ttcaaatgta ataaaagtat 2881 caacaaaaaa ttgttaatat acctctatac tttaacgtca aggagaaaaa accccggatc 2941 cgtaatacga ctcactatag ggcccgggat gcgtcaagtc tggttcagct ggattgtcgg 3001 tttgttcttg tgtttcttca acgtttcctc tgccgctcca gttaacacca ccactgaaga 3061 tgaaactgct caaattccag ctgaagcagt cattggttac tctgacttgg aaggtgactt 3121 cgatgttgcc gtcttgccat tctctaactc caccaacaac ggtttattat tcatcaacac 3181 tactattgct tccattgctg ctaaggaaga aggtgtttct ttggaaaaac gtgaagctga 3241 agctcaccat caccaccatc accaattgac taccgaatcc atgccattta acgtcgccga 3301 aggtaaagaa gttttgttgt tggttcacaa cttgccacaa caattattcg gctactcttg 3361 gtacaagggt gaaagagtcg atggtaacag acaaatcgtt ggttacgcta tcggtactca 3421 acaagctacc ccaggtccag ctaactctgg tagagaaacc atttacccga acgcttccct 3481 cttgatccaa aacgtcaccc aaaatgacac tggtttctac actttgcaag ttatcaagtc 3541 cgatttggtc aacgaagaag ctactggtca attccacgtt tacggtggtg gtggttccgg 3601 tggtggaggt tctggtggtg gtggttctgc tttgccaact aatggtacct ccactgaagc 3661 cccaactgac acaactaccg aagccccaac taccggtcta ccaaccaatg gtaccacttc 3721 cgctttccca ccaacgacct ctttacctcc atccaacact actacaactc caccatacaa 3781 cccatctaca gactacacta ccgattacac cgtcgtcact gaatacacca cttactgtcc 3841 agaaccaacc acttttacca ccaacggtaa gacttacacc gtcactgagc ctaccactct 3901 aactatcact gactgtccat gtactattga aaagcctaca accacttcaa ccactgaata 3961 taccgtcgta actgaatata ctacctactg tccagaacca acgaccttca ccaccaacgg 4021 taagacctat actgtcaccg aaccaaccac tcttaccatt actgattgcc catgtacgat 4081 cgaaaagcca accactactt ctaccactga atacaccgtt gttactgaat acaccaccta 4141 ctgtccagaa cctactacct tcactactaa cggtaagacc tacactgtta ctgaaccaac 4201 caccttgact atcaccgact gtccatgcac catcgaaaag tctgaagctc cagaatcttc 4261 ggttccagtt actgaatcaa agggtactac tactaaggaa accggtgtta ccaccaagca 4321 aactaccgct aacccatctt tgaccgtttc tactgtggtc ccagtttctt cttccgcttc 4381 ctctcactct gttgttatca acagcaatgg tgccaacgtt gttgttccag gtgctttggg 4441 tttggctggt gttgctatgt tgtttttgtg actcgagtaa gcttggtacc gcggctagct 4501 aagatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta 4561 tttttttata gttatgttag tattaagaac gttatttata tttcaaattt ttcttttttt 4621 tctgtacaga cgcgtgtacg catgtaacat tatactgaaa accttgcttg agaaggtttt 4681 gggacgctcg aagatccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 4741 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 4801 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 4861 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 4921 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 4981 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 5041 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 5101 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 5161 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 5221 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 5281 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 5341 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 5401 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 5461 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 5521 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 5581 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 5641 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 5701 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 5761 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 5821 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 5881 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 5941 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 6001 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 6061 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 6121 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 6181 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 6241 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 6301 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 6361 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 6421 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 6481 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 6541 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 6601 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 6661 cacatttccc cgaaaagtgc cacctgaacg aagcatctgt gcttcatttt gtagaacaaa 6721 aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac 6781 agaaatgcaa cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca tttttgtaaa 6841 acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac 6901 agaacagaaa tgcaacgcga gagcgctatt ttaccaacaa agaatctata cttctttttt 6961 gttctacaaa aatgcatccc gagagcgcta tttttctaac aaagcatctt agattacttt 7021 ttttctcctt tgtgcgctct ataatgcagt ctcttgataa ctttttgcac tgtaggtccg 7081 ttaaggttag aagaaggcta ctttggtgtc tattttctct tccataaaaa aagcctgact 7141 ccacttcccg cgtttactga ttactagcga agctgcgggt gcattttttc aagataaagg 7201 catccccgat tatattctat accgatgtgg attgcgcata ctttgtgaac agaaagtgat 7261 agcgttgatg attcttcatt ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta 7321 tatactacgt ataggaaatg tttacatttt cgtattgttt tcgattcact ctatgaatag 7381 ttcttactac aatttttttg tctaaagagt aatactagag ataaacataa aaaatgtaga 7441 ggtcgagttt agatgcaagt tcaaggagcg aaaggtggat gggtaggtta tatagggata 7501 tagcacagag atatatagca aagagatact tttgagcaat gtttgtggaa gcggtattcg 7561 caatatttta gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc 7621 agagcgcttt tggttttcaa aagcgctctg aagttcctat actttctaga gaataggaac 7681 ttcggaatag gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag 7741 ctgcgcacat acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat 7801 atatacatga gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc 7861 tatttatgta ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg 7921 ggtatcgtat gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa 7981 ttggattagt ctcatccttc aatgctatca tttcctttga tattggatca tactaagaaa 8041 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtc //