LOCUS sgSte2-Sst2-pML1 11150 bp DNA circular SYN 26-SEP-2025 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 11150) AUTHORS . TITLE Direct Submission JOURNAL Exported Oct 4, 2025 from SnapGene 8.1.1 https://www.snapgene.com FEATURES Location/Qualifiers source 1..11150 /mol_type="other DNA" /organism="recombinant plasmid" CDS 1..1719 /codon_start=1 /product="N-terminal portion of Streptococcus pyogenes Cas9 (Zetsche et al., 2015)" /label=Cas9(N) /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE" CDS 4114..4134 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" terminator 4162..4349 /gene="S. cerevisiae ADH1" /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" primer_bind complement(4217..4241) /label=pHybLex-R /note="ADH terminator, reverse primer" terminator complement(4467..4486) /label=SUP4 terminator /note="transcription terminator for the S. cerevisiae SUP4 tRNA gene" misc_RNA complement(4491..4552) /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" primer_bind complement(4518..4552) /label=载体.反向 (1) primer_bind 4538..4573 /label=sgRNA-R misc_RNA complement(4553..4565) /label=5' gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" primer_bind complement(4566..4585) /label=aSst2 tRNA complement(4586..4656) /label=tRNA-Gly(Ar) misc_RNA complement(4663..4738) /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" primer_bind complement(4691..4725) /label=载体.反向 (1) primer_bind 4711..4738 /label=sgRNA-R primer_bind complement(4739..4758) /label=aSte2 primer_bind complement(4740..4773) /label=sgRNA-F promoter complement(4759..5027) /label=SNR52 promoter /note="promoter for the S. cerevisiae small nucleolar RNA gene SNR52" primer_bind 4759..4786 /label=载体.正向 (1) primer_bind complement(5058..5078) /label=T3 /note="T3 promoter, forward primer" promoter complement(5058..5076) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(5097..5113) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5097..5113) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(5110..5132) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 5121..5137 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(join(5145..5151,5152..5169,5170..5175)) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 5190..5211 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(5328..5345) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5499..6087) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5579..5598) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(join(6258..7049,7050..7118)) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(6412..6421) /label=片段.反向 primer_bind 6881..6900 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(7119..7223) /gene="bla" /label=AmpR promoter rep_origin 7250..8592 /label=2u ori /note="yeast 2u plasmid origin of replication" protein_bind complement(8213..8260) /label=FRT /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." primer_bind 8633..8651 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(8689..8711) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 8811..8830 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" promoter 8854..8983 /gene="S. cerevisiae URA3" /label=URA3 promoter primer_bind complement(8954..8983) /label=载体.反向 primer_bind 8969..8999 /label=片段.正向 primer_bind 8984..8999 /label=引物 1 gene 9019..10375 /label=kanMX /note="yeast selectable marker conferring kanamycin resistance (Wach et al., 1994)" promoter 9019..9362 /label=TEF promoter /note="Ashbya gossypii TEF promoter" CDS 9363..10172 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin" /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(9430..9449) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" terminator 10178..10375 /label=TEF terminator /note="Ashbya gossypii TEF terminator" primer_bind complement(10395..10412) /label=引物 2 primer_bind complement(10396..10427) /label=片段.反向 primer_bind 10413..10443 /label=载体.正向 promoter 10450..11116 /gene="S. cerevisiae TDH3" /label=GAP promoter /note="promoter for glyceraldehyde-3-phosphate dehydrogenase; also known as the TDH3 promoter" primer_bind 10986..11009 /label=GPDpro-F /note="S. cerevisiae GPD promoter, forward primer" regulatory join(11145..11150,1..4) /label=Kozak sequence /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" BASE COUNT 3135 a 2592 c 2535 g 2888 t ORIGIN 1 atggacaaga agtattctat cggactggac atcgggacta atagcgtcgg gtgggccgtc 61 atcactgacg agtacaaggt gccctctaag aagttcaagg tgctcgggaa caccgaccgg 121 cattccatca agaaaaatct gatcggagct ctcctctttg attcagggga gaccgctgaa 181 gcaacccgcc tcaagcggac tgctagacgg cggtacacca ggaggaagaa ccggatttgt 241 taccttcaag agatattctc caacgaaatg gcaaaggtcg acgacagctt cttccatagg 301 ctggaagaat cattcctcgt ggaagaggat aagaagcatg aacggcatcc catcttcggt 361 aatatcgtcg acgaggtggc ctatcacgag aaatacccaa ccatctacca tcttcgcaaa 421 aagctggtgg actcaaccga caaggcagac ctccggctta tctacctggc cctggcccac 481 atgattaagt tcagaggcca cttcctgatc gagggcgacc tcaatcctga caatagcgat 541 gtggataaac tgttcatcca gctggtgcag acttacaacc agctctttga agagaacccc 601 atcaatgcaa gcggagtcga tgccaaggcc attctgtcag cccggctgtc aaagagccgc 661 agacttgaga atcttatcgc tcagctgccg ggtgaaaaga aaaatggact gttcgggaac 721 ctgattgctc tttcacttgg gctgactccc aatttcaagt ctaatttcga cctggcagag 781 gatgccaagc tgcaactgtc caaggacacc tatgatgacg atctcgacaa cctcctggcc 841 cagatcggtg accaatacgc cgaccttttc cttgctgcta agaatctttc tgacgccatc 901 ctgctgtctg acattctccg cgtgaacact gaaatcacca aggcccctct ttcagcttca 961 atgattaagc ggtatgatga gcaccaccag gacctgaccc tgcttaaggc actcgtccgg 1021 cagcagcttc cggagaagta caaggaaatc ttctttgacc agtcaaagaa tggatacgcc 1081 ggctacatcg acggaggtgc ctcccaagag gaattttata agtttatcaa acctatcctt 1141 gagaagatgg acggcaccga agagctcctc gtgaaactga atcgggagga tctgctgcgg 1201 aagcagcgca ctttcgacaa tgggagcatt ccccaccaga tccatcttgg ggagcttcac 1261 gccatccttc ggcgccaaga ggacttctac ccctttctta aggacaacag ggagaagatt 1321 gagaaaattc tcactttccg catcccctac tacgtgggac ccctcgccag aggaaatagc 1381 cggtttgctt ggatgaccag aaagtcagaa gaaactatca ctccctggaa cttcgaagag 1441 gtggtggaca agggagccag cgctcagtca ttcatcgaac ggatgactaa cttcgataag 1501 aacctcccca atgagaaggt cctgccgaaa cattccctgc tctacgagta ctttaccgtg 1561 tacaacgagc tgaccaaggt gaaatatgtc accgaaggga tgaggaagcc cgcattcctg 1621 tcaggcgaac aaaagaaggc aattgtggac cttctgttca agaccaatag aaaggtgacc 1681 gtgaagcagc tgaaggagga ctatttcaag aaaattgaat gcttcgactc tgtggagatt 1741 agcggggtcg aagatcggtt caacgcaagc ctgggtacct accatgatct gcttaagatc 1801 atcaaggaca aggattttct ggacaatgag gagaaagagg acatccttga ggacattgtc 1861 ctgactctca ctctgttcga ggaccgggaa atgatcgagg agaggcttaa gacctacgcc 1921 catctgttcg acgataaagt gatgaagcaa cttaaacgga gaagatatac cggatgggga 1981 cgccttagcc gcaaactcat caacggaatc cgggacaaac agagcggaaa gaccattctt 2041 gatttcctta agagcgacgg attcgctaat cgcaacttca tgcaacttat ccatgatgat 2101 tccctgacct ttaaggagga catccagaag gcccaagtgt ctggacaagg tgactcactg 2161 cacgagcata tcgcaaatct ggctggttca cccgctatta agaagggtat tctccagacc 2221 gtgaaagtcg tggacgagct ggtcaaggtg atgggtcgcc ataaaccaga gaacattgtc 2281 atcgagatgg ccagggaaaa ccagactacc cagaagggac agaagaacag cagggagcgg 2341 atgaaaagaa ttgaggaagg gattaaggag ctcgggtcac agatccttaa agagcacccg 2401 gtggaaaaca cccagcttca gaatgagaag ctctatctgt actaccttca aaatggacgc 2461 gatatgtatg tggaccaaga gcttgatatc aacaggctct cagactacga cgtggaccac 2521 atcgtccctc agagcttcct caaagacgac tcaattgaca ataaggtgct gactcgctca 2581 gacaagaacc ggggaaagtc agataacgtg ccctcagagg aagtcgtgaa aaagatgaag 2641 aactattggc gccagcttct gaacgcaaag ctaatcactc agcggaagtt cgacaatctc 2701 actaaggctg agaggggcgg actgagcgaa ctggacaaag caggattcat taaacggcaa 2761 cttgtggaga ctcggcagat tactaaacat gtagcccaaa tccttgactc acgcatgaat 2821 accaagtacg acgaaaacga caaacttatc cgcgaggtga aggtgattac cctgaagtcc 2881 aagctggtca gcgatttcag aaaggacttt caattctaca aagtgcggga gatcaataac 2941 tatcatcatg ctcatgacgc atatctgaat gccgtggtgg gaaccgccct aatcaagaag 3001 tacccaaagc tggaaagcga gttcgtgtac ggagactaca aggtctacga cgtgcgcaag 3061 atgattgcca aatctgagca ggagatcgga aaggccaccg caaagtactt cttctacagc 3121 aacatcatga atttcttcaa gaccgaaatc acccttgcaa acggtgagat ccggaagagg 3181 ccgctcatcg agactaatgg ggagactggc gaaatcgtgt gggacaaggg cagagatttc 3241 gctaccgtgc gcaaagtgct ttctatgcct caagtgaaca tcgtgaagaa aaccgaggtg 3301 caaaccggag gcttttctaa ggaatcaatc ctccccaagc gcaactccga caagctcatt 3361 gcaaggaaga aggattggga ccctaagaag tacggcggat tcgattcacc aactgtggct 3421 tattctgtcc tggtcgtggc taaggtggaa aaaggaaagt ctaagaagct caagagcgtg 3481 aaggaactgc tgggtatcac cattatggag cgcagctcct tcgagaagaa cccaattgac 3541 tttctcgaag ccaaaggtta caaggaagtc aagaaggacc ttatcatcaa gctcccaaag 3601 tatagcctgt tcgaactgga gaatgggcgg aagcggatgc tcgcctccgc tggcgaactt 3661 cagaagggta atgagctggc tctcccctcc aagtacgtga atttcctcta ccttgcaagc 3721 cattacgaga agctgaaggg gagccccgag gacaacgagc aaaagcaact gtttgtggag 3781 cagcataagc attatctgga cgagatcatt gagcagattt ccgagttttc taaacgcgtc 3841 attctcgctg atgccaacct cgataaagtc cttagcgcat acaataagca cagagacaaa 3901 ccaattcggg agcaggctga gaatatcatc cacctgttca ccctcaccaa tcttggtgcc 3961 cctgccgcat tcaagtactt cgacaccacc atcgaccgga aacgctatac ctccaccaaa 4021 gaagtgctgg acgccaccct catccaccag agcatcaccg gactttacga aactcggatt 4081 gacctctcac agctcggagg ggatgaggga gctcccaaga aaaagcgcaa ggtaggtagt 4141 tccggatcct agactctcga ggcgaatttc ttatgattta tgatttttat tattaaataa 4201 gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt aaaacgaaaa 4261 ttcttattct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt atagcatgag 4321 gtcgctctta ttgaccacac ctctaccggc atgccgagca aatgcctgca aatcgctccc 4381 catttctcta gagcggccgt ggtatcgttt agattggcaa ttacagtgtc ttagctcaca 4441 tgcttataac taattacatg actcgaagac ataaaaaaca aaaaaagcac caccgactcg 4501 gtgccacttt ttcaagttga taacggacta gccttatttt aacttgctat ttctagctct 4561 aaaactgttc tggcttagat ttctctgcgc aagcccggaa tcgaaccggg ggcccaacga 4621 tggcaacgtt ggattttacc actaaaccac ttgcgcttgt ttgcaccgac tcggtgccac 4681 tttttcaagt tgataacgga ctagccttat tttaacttgc tatttctagc tctaaaacca 4741 gttcatcagt agaggtgaga tcatttatct ttcactgcgg agaagtttcg aacgccgaaa 4801 catgcgcacc aactttcact tctacagcgt ttgaccaaaa tcttttgaac agaacattgt 4861 agggtgtgaa aaaatgcgca cctttaccgc tagcccaaga gggcactaca aaatctagag 4921 ttgtacttca aacgtacatg taatcacctt gtatatactc gaaagaaaac atcaagtttc 4981 tgtataaata tgagtgaaag cataatcata cattatcttt tcaaagattg tccgcggtgg 5041 agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca 5101 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 5161 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 5221 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 5281 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 5341 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 5401 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 5461 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 5521 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 5581 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 5641 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 5701 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 5761 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 5821 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 5881 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga 5941 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 6001 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 6061 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 6121 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 6181 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 6241 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 6301 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 6361 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 6421 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 6481 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 6541 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 6601 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 6661 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 6721 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 6781 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 6841 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 6901 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 6961 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 7021 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 7081 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 7141 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 7201 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga acgaagcatc 7261 tgtgcttcat tttgtagaac aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa 7321 tctgagctgc atttttacag aacagaaatg caacgcgaaa gcgctatttt accaacgaag 7381 aatctgtgct tcatttttgt aaaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca 7441 aagaatctga gctgcatttt tacagaacag aaatgcaacg cgagagcgct attttaccaa 7501 caaagaatct atacttcttt tttgttctac aaaaatgcat cccgagagcg ctatttttct 7561 aacaaagcat cttagattac tttttttctc ctttgtgcgc tctataatgc agtctcttga 7621 taactttttg cactgtaggt ccgttaaggt tagaagaagg ctactttggt gtctattttc 7681 tcttccataa aaaaagcctg actccacttc ccgcgtttac tgattactag cgaagctgcg 7741 ggtgcatttt ttcaagataa aggcatcccc gattatattc tataccgatg tggattgcgc 7801 atactttgtg aacagaaagt gatagcgttg atgattcttc attggtcaga aaattatgaa 7861 cggtttcttc tattttgtct ctatatacta cgtataggaa atgtttacat tttcgtattg 7921 ttttcgattc actctatgaa tagttcttac tacaattttt ttgtctaaag agtaatacta 7981 gagataaaca taaaaaatgt agaggtcgag tttagatgca agttcaagga gcgaaaggtg 8041 gatgggtagg ttatataggg atatagcaca gagatatata gcaaagagat acttttgagc 8101 aatgtttgtg gaagcggtat tcgcaatatt ttagtagctc gttacagtcc ggtgcgtttt 8161 tggttttttg aaagtgcgtc ttcagagcgc ttttggtttt caaaagcgct ctgaagttcc 8221 tatactttct agagaatagg aacttcggaa taggaacttc aaagcgtttc cgaaaacgag 8281 cgcttccgaa aatgcaacgc gagctgcgca catacagctc actgttcacg tcgcacctat 8341 atctgcgtgt tgcctgtata tatatataca tgagaagaac ggcatagtgc gtgtttatgc 8401 ttaaatgcgt acttatatgc gtctatttat gtaggatgaa aggtagtcta gtacctcctg 8461 tgatattatc ccattccatg cggggtatcg tatgcttcct tcagcactac cctttagctg 8521 ttctatatgc tgccactcct caattggatt agtctcatcc ttcaatgcta tcatttcctt 8581 tgatattgga tcatactaag aaaccattat tatcatgaca ttaacctata aaaataggcg 8641 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat 8701 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 8761 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga 8821 gcagattgta ctgagagtgc accataccac cttttcaatt catcattttt tttttattct 8881 tttttttgat ttcggtttcc ttgaaatttt tttgattcgg taatctccga acagaaggaa 8941 gaacgaagga aggagcacag acttagattg gtatatatac gcaagcttgc ctcgtccccg 9001 ccgggtcacc cggccagcga catggaggcc cagaataccc tccttgacag tcttgacgtg 9061 cgcagctcag gggcatgatg tgactgtcgc ccgtacattt agcccataca tccccatgta 9121 taatcatttg catccataca ttttgatggc cgcacggcgc gaagcaaaaa ttacggctcc 9181 tcgctgcaga cctgcgagca gggaaacgct cccctcacag acgcgttgaa ttgtccccac 9241 gccgcgcccc tgtagagaaa tataaaaggt taggatttgc cactgaggtt cttctttcat 9301 atacttcctt ttaaaatctt gctaggatac agttctcaca tcacatccga acataaacaa 9361 ccatgggtaa ggaaaagact cacgtttcga ggccgcgatt aaattccaac atggatgctg 9421 atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc 9481 gattgtacgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg 9541 ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc 9601 cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc 9661 ccggcaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg 9721 atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta 9781 acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg 9841 atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa 9901 tgcataagct tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg 9961 ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa 10021 tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt 10081 cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc 10141 agtttcattt gatgctcgat gagtttttct aatcagtact gacaataaaa agattcttgt 10201 tttcaagaac ttgtcatttg tatagttttt ttatattgta gttgttctat tttaatcaaa 10261 tgttagcgtg atttatattt tttttcgcct cgacatcatc tgcccagatg cgaagttaag 10321 tgcgcagaaa gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta tactgctgtc 10381 gattcgatac taacgccgcc tccagtgtcg aatgatatcg aattcagtct actagtcagt 10441 tcgagtttat cattatcaat actgccattt caaagaatac gtaaataatt aatagtagtg 10501 attttcctaa ctttatttag tcaaaaaatt agccttttaa ttctgctgta acccgtacat 10561 gcccaaaata gggggcgggt tacacagaat atataacatc gtaggtgtct gggtgaacag 10621 tttattcctg gcatccacta aatataatgg agcccgcttt ttaagctggc atccagaaaa 10681 aaaaagaatc ccagcaccaa aatattgttt tcttcaccaa ccatcagttc ataggtccat 10741 tctcttagcg caactacaga gaacaggggc acaaacaggc aaaaaacggg cacaacctca 10801 atggagtgat gcaacctgcc tggagtaaat gatgacacaa ggcaattgac ccacgcatgt 10861 atctatctca ttttcttaca ccttctatta ccttctgctc tctctgattt ggaaaaagct 10921 gaaaaaaaag gttgaaacca gttccctgaa attattcccc tacttgacta ataagtatat 10981 aaagacggta ggtattgatt gtaattctgt aaatctattt cttaaacttc ttaaattcta 11041 cttttatagt tagtcttttt tttagtttta aaacaccaag aacttagttt cgaataaaca 11101 cacataaaca aacaaaagat ctgatatgga tcgaattaga tctcgccacc //