Molecular Cloning

Molecular cloning chapter describes all of the construction of plasmid in our program This chapter includes:

1. Plasmid extraction
2. Agarose gel electrophoresis
3. Gel extraction
4. Preparation of Bacillus subtilis Competent Cells
5. Transformation
6. Plasmids Construction by homologous recombination

sinR-tasA Knock Out

Adhesion Module

This chapter includes:

1. Protein Expression
2. Biofilm Induction

Function Test Module

This chapter includes:

1. Verification of the epcadR-pcadR-mcherry sensing module
2. Verification of tasA-smtA adsorption module
3. Verification of the epcadR-pcadR-mcherry-tasA-mfp5 sensing-adhesion module
4. Verification of tasA-smtA adsorption-adhesion module

Safety Module

This chapter includes:

1. Operon efficiency verification