Welcome to Our Project
We are the WLSA-China iGEM team, a group of passionate students dedicated to advancing synthetic biology for real-world challenges. Our project, APOPTO-SENSE 2.0, introduces an innovative biosensing platform for personalized cancer treatment, focusing on rapid detection of drug-induced apoptosis to guide chemotherapy decisions.
Explore our work below, from project details to team contributions, and join us in revolutionizing oncology!
Project Summary
APOPTO-SENSE 2.0 is a dual-cell system that detects apoptosis in tumor cells exposed to chemotherapy, providing quantitative insights for personalized medicine. Using engineered synNotch receptors in sensor cells, it converts phosphatidylserine exposure into fluorescent signals, enabling fast, cost-effective drug sensitivity testing. Our proof-of-concept validates its potential for leukemia and beyond, with expansions to multiplexed cell death detection.
Project Schematic
This schematic illustrates the dual-cell workflow: Target tumor cells are treated with drugs, inducing apoptosis and PS exposure. Sensor cells detect PS via Annexin V-synNotch, triggering Gal4-VP64 release and TagBFP expression, normalized to mCherry for quantification.