Despite reaching historic highs in global total food production, the issue of hunger remains severe. The spoilage and deterioration of food exacerbate food waste and also pose significant safety hazards.
➤ In 2024, more than 295 million people worldwide still faced severe hunger.
➤ In 2023, approximately 600 million people globally suffered from foodborne diseases annually.
➤ Children under the age of five account for 40% of the cases, leading to about 125,000 child fatalities each year.

As an essential component of the human diet, aquatic products are celebrated for their substantial nutritional value, distinctive flavor, and variety.

➤ In 2019, seafoods topped global consumption charts, surpassing all other meat types.
➤ In 2022, global fisheries and aquaculture production amounted to 185.4 million tons.
➤ By 2030, it is estimated that the global annual production of aquatic animals will reach 205 million tons, a fivefold increase over the 1960 level.

In 2019, more aquatic foods were consumed globally than chicken, pork, beef or sheep.

More than three billion people worldwide depend on aquatic products for at least one-fifth of their animal protein intake. China stands as the world's largest producer of aquatic products, accounting for approximately 70% of global aquaculture output. In 2024, China's total production of aquatic products amounted to 73.66 million tons, representing a 3.5% increase from the previous year. This growth underscores the vital role of aquatic products in the diets of Chinese residents.

The spoilage of aquatic products poses a significant challenge that urgently needs to be addressed in the field of food safety.

Aquatic products are known for their short shelf life and highly perishable property, with rapid quality deteriorating after harvest. Research suggests that the annual loss and waste of aquatic products amount to 15% (23.8 million tons in 2021), with 30%-35% of this loss occurring during transportation and processing. Spoilage is identified as a major contributing factor to this issue. Moreover, spoiled aquatic products accumulate histamine toxins and harbor pathogenic bacteria, potentially leading to various foodborne diseases. In 2023, aquatic products were responsible for 379 foodborne outbreaks in Chinese mainland, affecting 2,061 individuals.

In 2023, aquatic products caused 379 foodborne outbreaks, affecting 2,061 individuals.

We urgently need a safer, more efficient, and environmentally friendly biological preservation technology!
Currently, the preservation of aquatic products mainly relies on two methods: chemical preservatives and cold chain systems. Chemical preservatives, including sulfites, are associated with carcinogenic risks and potential harm to human health. Meanwhile, the fossil fuel-dependent cold chain systems are a major source of carbon emissions and plastic pollution, thereby causing substantial environmental damage. The production of one ton of aquatic products directly generates 319.71 kg of CO2. Furthermore, current cold chain systems are responsible for 402 billion kg of CO2 emissions globally.

Contrary to their seemingly useless appearance, crab and shrimp shells possess immense potential, acting as a natural preservative!

Globally, the massive waste of crab and shrimp shell resources represents a significant problem. In 2022, China produced over 50 million tons of marine crabs and shrimp, resulting in approximately 10 million tons of crab and shrimp shell. Notably, some aquaculture practitioners directly discard these shells in nearshore areas.

The primary component of crab and shrimp shells is chitin, which ranks as the world's second largest natural biomass resource with an annual biosynthetic capacity of approximately 10 billion tons. Through deacetylation and further chemical processing, chitin can be converted into chitosan, which can be further transformed into chitooligosaccharides, a broad-spectrum and highly effective antibacterial agent.

Chemical methods for degrading chitosan typically utilize acids or oxidizing agents. However, these approaches often lead to excessive hydrolysis, yielding poorly controlled product structures and contributing to sewage pollution. Physical methods, such as microwave radiation, ultrasonic fragmentation, or turbulent cavitation, are employed but encounter challenges including high equipment demands and difficulties in accurately controlling the degradation process.

OUR SOLUTION
C R U S T A

We are dedicated to exploring a safer, more efficient and enviromentally friendly alternative.

Inspired by the discovery that chitooligosaccharides with higher degrees of polymerization possesses enhanced antibacterial properties, we designed experiments to screen chitosanases capable of producing degradation products with superior antibacterial efficacy. We further developed protocols to semi-rational design signal peptides with better secretion effect , intending to link these peptides with the most effective chitosanase mutants and bypass the protein purification step. We successfully achieved extracellular chitosan degradation using a novel signal peptide LMT C14A, enhancing the antibacterial performance of the degradation products by 7.96-fold compared to the wild-type.

We believe that transforming "shell waste" into valuable resources will not only protect our food safety but also establish a sustainable new pathway for environmental protection, energy conservation, emission reduction, and the circular economy.

Say goodbye to traditional chemical preservatives!

We have developed an innovative, green, healthy, and sustainable high-efficiency biological preservative centered around chitooligosaccharides, effectively embodying the principle of "sourced from aquatic products, used for aquatic products." This approach facilitates a circular economy for crab and shrimp shell resources through a cycle of recycling, processing, reutilization.

Chitooligosaccharides, serving as a natural preservative, effectively inhibits numerous spoilage bacteria, including Escherichia coli, Vibrio parahaemolyticus, Staphylococcus aureus, and Pseudomonas putida. It disrupts cell membrane integrity, induces content leakage, and interferes with the electron transport chain and the TCA cycle.