Overview
To produce size-tunable PHA microspheres, we conducted a series of experiments covering plasmid and strain construction, protein expression, growth curve test, fluorescence microscopy for PHA observation, TEM examination, forming a complete experimental record.
Cycle 1: phaC and phaCAB
Cycle 2a: phaC (phaCAB)-PcspA-pha
Cycle 2b: phaC (phaCAB)-PLight-phaP
Cycle 3: phaC (phaCAB) + ADF
Fluorescence microscopy for PHA observation
TEM examination of PHA morphology