Parts
This page details the new basic and composite parts developed by the HongKong-HS Team for the detection of colorectal cancer (CRC) using miRNA-based toehold switches.
New Basic Parts
The following basic parts are regulatory toehold switches designed to detect specific miRNAs (miRNA-92a-3p and miRNA-135b-5p) upregulated in the faeces of colorectal cancer patients.
Part ID
Type
Name
Description
Regulatory
Toehold switch 1 for detection of miRNA-92a-3p
Contains a region complementary to miRNA-92a-3p, which is upregulated in the faeces of patients with colorectal cancer.
Regulatory
Toehold switch 2 for detection of miRNA-92a-3p
Contains a region complementary to miRNA-92a-3p, which is upregulated in the faeces of patients with colorectal cancer.
Regulatory
Toehold switch 3 for detection of miRNA-92a-3p
Contains a region complementary to miRNA-92a-3p, which is upregulated in the faeces of patients with colorectal cancer.
Regulatory
Toehold switch 7 for detection of miRNA-135b-5p
Contains a region complementary to miRNA-135b-5p, which is upregulated in the faeces of patients with colorectal cancer.
Regulatory
Toehold switch 8 for detection of miRNA-135b-5p
Contains a region complementary to miRNA-135b-5p, which is upregulated in the faeces of patients with colorectal cancer.
Regulatory
Toehold switch 9 for detection of miRNA-135b-5p
Contains a region complementary to miRNA-135b-5p, which is upregulated in the faeces of patients with colorectal cancer.
Table 1. New Basic Parts for miRNA Detection
New Composite Parts
The following composite parts are devices integrating toehold switches with T7 promoter and terminator, and a eGFP reporter, designed to convert miRNA detection into a fluorescent signal for colorectal cancer screening.
Part ID
Type
Name
Description
Device
Toehold switch 1 for detection of miRNA-92a-3p with T7 promoter and terminator, and eGFP reporter
Consists of a toehold domain, regulatory hairpin, and eGFP reporter, functioning as a molecular switch that converts miRNA-92a-3p detection into a fluorescent protein signal.
Device
Toehold switch 2 for detection of miRNA-92a-3p with T7 promoter and terminator, and eGFP reporter
Consists of a toehold domain, regulatory hairpin, and eGFP reporter, functioning as a molecular switch that converts miRNA-92a-3p detection into a fluorescent protein signal.
Device
Toehold switch 3 for detection of miRNA-92a-3p with T7 promoter and terminator, and eGFP reporter
Consists of a toehold domain, regulatory hairpin, and eGFP reporter, functioning as a molecular switch that converts miRNA-92a-3p detection into a fluorescent protein signal.
Device
Toehold switch 7 for detection of miRNA-135b-5p with T7 promoter and terminator, and eGFP reporter
Consists of a toehold domain, regulatory hairpin, and eGFP reporter, functioning as a molecular switch that converts miRNA-135b-5p detection into a fluorescent protein signal.
Device
Toehold switch 8 for detection of miRNA-135b-5p with T7 promoter and terminator, and eGFP reporter
Consists of a toehold domain, regulatory hairpin, and eGFP reporter, functioning as a molecular switch that converts miRNA-135b-5p detection into a fluorescent protein signal.
Device
Toehold switch 9 for detection of miRNA-135b-5p with T7 promoter and terminator, and eGFP reporter
Consists of a toehold domain, regulatory hairpin, and eGFP reporter, functioning as a molecular switch that converts miRNA-135b-5p detection into a fluorescent protein signal.
Table 2. New Composite Parts for miRNA Detection