Epigenetic Programmable Intervention and Control
Beyond Hardware - Unlocking the Genomic Operating System
We present dCas9-dam - our fusion protein with gRNA capable of making precise epigenetic edits on the genome.
What is EPIC?
EPIC is a CRISPR-based toolkit that converts bacterial DNA methylation from a global, untargeted process into a programmable, site-specific regulatory tool.
Why this is not "just another expression regulating tool"
Gene expression control in bacteria is already well-established, and EPIC does not aim to outperform existing tuning methods. Instead, it introduces an entirely new dimension to genetic engineering, one that leverages epigenetic memory to achieve programmable and heritable control over expression.
The Guide:
Guide RNA sequences direct the EPIC system to any specific genomic site.
The Rewrite:
The dCas9-Dam complex methylates the targeted sequence.
The Result:
This targeted methylation rewrites the gene expression: selectively activating or silencing the gene with unrivaled precision.
Precise Targeting
Our engineered dCas9-Dam system provides a novel pipeline for DNA methylation targeting.
Controlled Methylation
The fusion protein enables selective epigenetic modification without altering the DNA sequence.
Potential Applications
Biomanufacturing
Precise epigenetic modifications for therapeutic applications, enabling targeted gene expression control without permanent DNA alterations for treating genetic disorders.
Epigenetic Memory Systems & Biosensors
Single-trigger “lock-in” circuits translate transient environmental signals into lasting expression states, forming the basis for self-sustaining biosensors and adaptive bioprocess control.
Therapeutics
Targeted methylation and demethylation can reversibly modulate disease-linked genes, offering precision control for regenerative medicine, metabolic reprogramming, and next-generation cell-based therapies.
