In order to ensure LarVase's long-term viability, we have developed a five-year business roadmap. The five-year horizon reflects the typical timeline for a biotech product to progress from lab validation to full market integration. This roadmap serves as a precise tool to manage the time bound development while proactively addressing both the positive and negative long-term impacts of our solution.

Year 1

Laboratory Validation and Enzyme Purification Protocols

• Optimize and scale up the YebF-assisted secretion system in E. coli to maximize cellulase yield and purity.
• Establish standard operating procedures for enzyme purification without residual GMOs.
• Begin initial dialogue with Taiwan FDA regarding classification as an industrial enzyme, not a food additive or GMO.
• Identify and secure partnerships with BSFL farms for pilot-scale application.

Year 2

Field Trials and Environmental Testing

• Deploy field trials at BSFL farms using varied food waste substrates and regional conditions.
• Measure performance indicators: decomposition speed, larvae biomass, oil content, and methane emission reduction.
• Begin application for registration as a biotechnological agent under Taiwan's Environmental Agents Control Act or Agricultural Chemicals Regulation if applicable.
• File for intellectual property protection on the production method and formulation.
• Begin Taiwan Small Business Innovation Research (SBIR) grant application for industrial scale-up.

Year 3

Regulatory Submission and Pilot-Scale Manufacturing

• Submit enzyme product application to Taiwan EPA under "industrial enzyme" classification and to COA for agricultural waste treatment use, in compliance with current microbial product guidelines.
• Confirm non-GMO classification under Taiwan FDA biosafety guidelines and notify the National Biotechnology Research Park.
• Construct pilot-scale manufacturing units within an approved science park zone to comply with industrial wastewater, labor, and GMP standards.
• Apply for regulatory pathway clarification in one export market (e.g., Vietnam, Thailand) through local legal counsel.

Year 4

Controlled Market Release and Post-Market Surveillance

• Begin controlled commercial sales to registered BSFL operators within Taiwan under existing waste processing and agri-feed regulations.
• Implement legal contracts covering liability, application instructions, and limits of use.
• Collect user performance data and conduct third-party verification of biodegradation metrics under ISO 17025-certified labs.
• Monitor compliance with Taiwan's Industrial Waste Disposal Act and document the enzyme impact on BSFL residues used in feed or soil.

Year 5

Regional Expansion and Legal Risk Management

• File for product registration or recognition in two ASEAN markets with defined circular economy or insect protein policies.
• Begin documentation for compliance with OECD Test Guidelines (Sections 4 and 5) and FAO enzyme evaluation standards.
• Procure liability insurance and draft licensing contracts covering domestic use and limited regional exports.