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Lab / Wet lab / Parts
Parts
Basic Parts
These basic parts encode the genetic sequences responsible for producing our antimicrobial peptides (AMPs), which were screened for activity against Phytophthora. In addition, some parts were designed to facilitate downstream purification of the expressed peptides.
| Part code | PhytoBLOCK name | Part name | Usage |
|---|---|---|---|
| BBa_25DO0ICK | pPB011 | Vicilin-like antimicrobial peptides 2-2 | Antimicrobial peptide (AMP) |
| BBa_258YJPQ3 | pPB012 | MiAMP2c-2 | Antimicrobial peptide (AMP) |
| BBa_25CH9T79 | pPB013 | Cecropin | Antimicrobial peptide (AMP) |
| BBa_25N58AWT | pPB014 | Lucimycin | Antimicrobial peptide (AMP) |
| BBa_25OQ2MUK | pPB015 | Ee-CBP (Leaves) | Antimicrobial peptide (AMP) |
| BBa_25CSAZHD | pPB016 | Antifungal peptide 2 | Antimicrobial peptide (AMP) |
| BBa_25LAH38O | pPB017 | Antifungal peptide 1 | Antimicrobial peptide (AMP) |
| BBa_2590DQBK | pPB020 | HIS-SUMO | Purification |
| BBa_25FN8UNC | pPB057 | HIS-Thioredoxin-TEV | Purification |
Composite Parts
These are the final genetic constructs we assembled to evaluate in our competition assays or to purify our antimicrobial peptides (AMPs).
| Part code | PhytoBLOCK name | Part name | Image of composite part |
|---|---|---|---|
| BBa_25XJTLJR | pPB060 | Xyl inducible - HIS_SUMO - Cecropin |
|
| BBa_257U8BE6 | pPB034 | Xyl inducible - Vicilin-like antimicrobial peptides 2-2, no secretion tag |
|
| BBa_259SUTIE | pPB036 | Xyl inducible - Cecropin, no secretion tag |
|
| BBa_2536XRFR | pPB027 | Xyl inducible - Vicilin-like antimicrobial peptides 2-2 |
|
| BBa_257MKPPE | pPB029 | Xyl inducible - Cecropin (AMP) |
|
Summary
Our parts collection consists of both basic and composite parts designed for antimicrobial peptide production and purification. The basic parts encode individual AMPs and purification tags, while the composite parts represent complete genetic constructs for expression and evaluation in Bacillus subtilis.