Medium Bacterial culture Solution & Buffers Preparation of Competent cells E.Coli Transformation MiniPrep for PCR and Sanger Preparation for Sanger Sequencing PCR Storaged of Transformed Bacteria Extraction of Vesicules (A) Protein Purification (Batch Method, B) Protein Purification from Vesicules (C) Protein Purification of the cell pellet Protein quantification (Thermoscientific) SDS Page Colorimetric monitoring