Basic Parts

MESA Receptor Domains

NameRegistry LinkType
C-terminal domain of split TEV protease (190K)BBa_2560Y492Coding
N-terminal domain of split TEV protease (75E)BBa_25PZ44LECoding
N-terminal domain of split TEV protease (75S)BBa_25OY72JOCoding
TEV protease cleavage site (ENLYFQ*M)BBa_25V42NXGCoding
Murine CD4 Signal Peptide (Mus musculus)BBa_25VVSW84Coding
human CD28 transmembrane domainBBa_25ISZYYLCoding
LaM2 anti-mCherry nanobodyBBa_25UPRPBJCoding
LaM4 anti-mCherry nanobodyBBa_25SRZEC5Coding
Canonical mTagBFP2BBa_25H9MN2SCoding
TetR (Tetracycline repressor protein class B from transposon Tn10)BBa_25OTY7CMCoding
tTA (Tetracycline-controlled Transactivator)BBa_253I26J2Coding
rtTA (Reverse tetracycline-controlled transactivator)BBa_25HLLTKZCoding
FKBPBBa_25KGEVF4Coding
FRBBBa_257TOK4ACoding
Synthetic progesterone binderBBa_25XUYMDNCoding
Antibody-derived progesterone complex binderBBa_25IFCMISCoding
Protein A-like Fc binderBBa_25ZXW5UVCoding
FcγRI (CD64)-derived Fc binderBBa_2546ZOJHCoding

Encapsulin Nanocages Components

NameRegistry LinkType
FKBP12-derived destabilizing domain (DD)BBa_25SD6973Coding
Myxococcus xanthus peptide tag for encapsulin loading (MxSig)BBa_25W7WIJFCoding
Thermagota maritima peptide tag for encapsulin loading (TmSig)BBa_25NSVMV1Coding
Quasibacillus thermotolerans peptide tag for encapsulin loading (QtSig)BBa_25KL9UC2Coding
Thermagota maritima MSB8 encapsulin shell proteinBBa_25O3XMCHCoding
Myxococcus xanthus DK1622 encapsulin shell protein (EncA)BBa_25IGMI8HCoding
Quasibacillus thermotolerans family 1 encapsulin shell proteinBBa_25GB07WOCoding
eUnaG fluorescent proteinBBa_25I6WGWICoding
HIV-1 Rev nuclear export signalBBa_2574B2SRCoding
Lanthanide-binding tagBBa_25A9CJANCoding
Myxococcus xanthus encapsulin cargo protein EncDBBa_25CGE7NXCoding

Tyrosinases

NameRegistry LinkType
Bacillus megaterium tyrosinase (BmTyr)BBa_25JTFYB9Coding
Bacillus megaterium tyrosinase (BmTyr) E195S A221VBBa_25PQQECLCoding
Bacillus megaterium tyrosinase (BmTyr) R209S M215E V218MBBa_252OBJXCCoding
Bacillus megaterium tyrosinase (BmTyr) G43R M61H F197W Q214D V217A A232CBBa_25MM9JBECoding
Circularly permuted Bacillus megaterium tyrosinase (cp102BmTyr)BBa_252MT4O5Coding
N-terminal domain of split Bacillus megaterium tyrosinase (85)BBa_2502R9NPCoding
C-terminal domain of split Bacillus megaterium tyrosinase (85)BBa_25LKZLQDCoding
N-terminal domain of split Bacillus megaterium tyrosinase (157)BBa_25O84IGRCoding
C-terminal domain of split Bacillus megaterium tyrosinase (157)BBa_25JLEAQECoding
N-terminal domain of split Bacillus megaterium tyrosinase (201)BBa_25UROWUHCoding
C-terminal domain of split Bacillus megaterium tyrosinase (201)BBa_25JQXA9PCoding
Laceyella sacchari tyrosinase (LsTyr)BBa_25714KA6Coding
Streptomyces kathirae SC-1 tyrosinase caddie protein (MelC1) Δ(1–32)BBa_2532YNLHCoding
Streptomyces kathirae SC-1 tyrosinase (MelC2)BBa_25LVMO1CCoding
Streptomyces avermitilis MA4680 tyrosinase caddie protein (MelC1) Δ(1–26)BBa_25RLK06SCoding
Streptomyces avermitilis MA4680 tyrosinase caddie protein (MelC1) Δ(1–26) Y92FBBa_25KHE0R2Coding
Streptomyces avermitilis MA4680 tyrosinase (MelC2)BBa_25ZYJJ2DCoding
Streptomyces avermitilis MA4680 tyrosinase (MelC2) I42YBBa_25CYED64Coding
Hahella sp. CCB-MM4 tyrosinase (HcTyr) Δ(297-335)::GENLYFQMGBBa_25OLOGCUCoding
Hahella sp. CCB-MM4 tyrosinase (HcTyr) Δ(297-466)::GENLYFQBBa_25618YX2Coding
Verrucomicrobium spinosum tyrosinase (VsTyr) Δ(1–36) Δ(358-370)::GSGSENLYFQMGSGSBBa_255HVVDUCoding
Verrucomicrobium spinosum tyrosinase (VsTyr) Δ(1–36) Δ(358-518)::GSGSENLYFQBBa_25WNZKAFCoding

Kill Switch Components

NameRegistry LinkType
Dimerization Domain B (FKBP(F36V) / DmrB)BBa_25W20DZXCoding
Inducible Caspase-9 (iCasp9)BBa_25ZBH023Coding

Miscellaneous Parts Components

NameRegistry LinkType
P3 Coiled-Coil PeptideBBa_255JAVI1Coding
P4 Coiled-Coil PeptideBBa_2533X4WPCoding
T2ABBa_25QHFMFICoding
HA tagBBa_25ZKXVV7Coding
Strep-tag IIBBa_25GJLC0ECoding
CSL tagBBa_25E2YYCECoding
Factor Xa cleavage siteBBa_2552E37ICoding
Enhanced green fluorescent proteinBBa_25Z3HGCFCoding
mCherry RFPBBa_J18932Protein_Domain
Twin-Strep TagBBa_K3211600Miscellaneous
hexahistidine-tagBBa_K1362468Miscellaneous
Myc tagBBa_2556GYXQCoding
TEV proteaseBBa_25GP3L7ZCoding

Linkers

NameRegistry LinkType
GS6 linkerBBa_25M8MAFJCoding
(G4S)2 linkerBBa_25C31LV0Coding
GC6 Linker (GGGCGG)BBa_259K2OSNCoding
G2TG2S Linker (GGTGGS)BBa_25U53QEDCoding
(G4S)3 LinkerBBa_251SX194Coding
(GGS)6 LinkerBBa_252HNKS3Coding
LKGS LinkerBBa_257QF9SFCoding
GGS linkerBBa_25C5UWV5Coding
GS2EG2 linkerBBa_259OI0VNCoding
GSG4S linkerBBa_251VIC9UCoding
GSG3 linkerBBa_25ADA82ZCoding
Alternative GSG3 linkerBBa_25J06KDYCoding
GT(G3S)2 linkerBBa_254TRZBUCoding
Flexible linkerBBa_25X9X8TXCoding
SG3S linkerBBa_25RCCSCJCoding
SG linkerBBa_25TJN7KOCoding
GSG4S linkerBBa_25Y1JSSSCoding
GSG linkerBBa_256RR95NCoding
(G3S)3 linkerBBa_25VCH2CBCoding
(G3S)5 linkerBBa_25QYUEOICoding
(G3S)2 linkerBBa_25ZQDR5GCoding
(G3S)7 linkerBBa_25NMUUM5Coding

Regulatory Components

NameRegistry LinkType
Full length human cytomegalovirus (CMV) immediate-early promoter/enhancerBBa_25UHYKHZRegulatory
CMV PromoterBBa_K2217006Regulatory
Canonical CMV 5’ UTRBBa_25FE6ZLURegulatory
Herpes Simplex Virus Thymidine Kinase (TK) polyadenylation signalBBa_258L1MBOTerminator
T7 PromoterBBa_K4344066Regulatory
Terminator (T7 RNAP specific, T_Phi)BBa_B0016Terminator
CMV enhancerBBa_K5102067Regulatory
Kozak Sequence (Human RBS)BBa_K4046050RBS
Woodchuck posttranscriptional regulatory element (WPRE)BBa_K5102069Regulatory
double stopBBa_K2680563Miscellaneous
T7 RBSBBa_K3257011RBS
LacOBBa_K1624002Regulatory
strong rbs siteBBa_K2967001RBS
TetOBBa_K2557038DNA
minCMV promoterBBa_K2446053Regulatory
bGH poly(A) signalBBa_K4251016Signalling
Simian virus 40 PolyA (SV40 Poly(A))BBa_K5253006Terminator
EF-1α promoterBBa_25QOS6K7Regulatory

Composite Parts

MESA Receptors

NameRegistry LinkType
LaM4 anti-mCherry MESA with N-TEV 75EBBa_25GNFI3UCoding
LaM4 anti-mCherry MESA with N-TEV 75SBBa_254S3E5NCoding
LaM2 anti-mCherry MESA with C-TEV 190KBBa_258AY584Coding
LaM2 anti-mCherry MESA with C-TEV 190K & cleavable BFPBBa_25TB04SFCoding
LaM2 anti-mCherry MESA with C-TEV 190K & cleavable tTA-BFPBBa_25K0CCJ6Coding
LaM2 anti-mCherry MESA with C-TEV 190K & cleavable rtTA-BFPBBa_255MHWYSCoding
LaM2 anti-mCherry MESA with C-TEV 190K & cleavable TetR-BFPBBa_252Y5GHYCoding
Soluble rapamycin-inducible MESA (N-TEV 75E)BBa_25FOITIVCoding
Soluble rapamycin-inducible MESA (N-TEV 75S)BBa_25A91G3QCoding
TEV-releasable membrane tethered tetracycline-controlled transactivatorBBa_255QL5HACoding
Soluble progesterone-inducible MESABBa_25636P3CCoding
IgG-sensing MESA receptorBBa_25ZUH1R6Coding
IgG-sensing MESA receptor with TEV-releasable proteaseBBa_25QFZDG9Coding

Encapsulin Nanocages

NameRegistry LinkType
TEV-inducible Myxococcus xanthus encapsulin assembly (via LBT blockade)BBa_25KXAEJDCoding
TEV-inducible Quasibacillus thermotolerans encapsulin assembly (via LBT blockade)BBa_25MF5YOECoding
TEV-inducible Thermogota maritima encapsulin assembly (via LBT blockade)BBa_256VHGCFCoding
MxEnc BmTyr synthetic melanosome cargo unitBBa_257HDO36Coding
QtEnc BmTyr synthetic melanosome cargo unitBBa_25R1IAMMCoding
TmEnc BmTyr synthetic melanosome cargo unitBBa_25OJ1IHECoding
MxEnc Synthetic Melanosome Shell UnitBBa_25H001GLCoding
QtEnc Synthetic Melanosome Shell UnitBBa_25ZUPYADCoding
TmEnc Synthetic Melanosome Shell UnitBBa_25EXP1JFCoding
MxEnc-BmTyr synthetic melanosome subunitBBa_25GM240TCoding
QtEnc-BmTyr synthetic melanosome subunitBBa_25RJFKZUCoding
TmEnc-BmTyr synthetic melanosome subunitBBa_255ZXR8TCoding
Myxococcus xanthus DK1622 encapsulin shell protein (EncA) expressionBBa_2539NWY1Coding
MxEncD BmTyr synthetic melanosome cargo unitBBa_25D4J4ISCoding
TEV protease expressionBBa_25JUMZW7Coding

Cell-free Expression of Tyrosinases

NameRegistry LinkType
Bacillus megaterium tyrosinase (BmTyr) for cell-free expressionBBa_256CVVJRCoding
Bacillus megaterium tyrosinase (BmTyr) E195S A221V for cell-free expressionBBa_25LRVPGGCoding
Bacillus megaterium tyrosinase (BmTyr) R209S M215E V218M for cell-free expressionBBa_25NYZ63ECoding
Bacillus megaterium tyrosinase (BmTyr) G43R M61H F197W Q214D V217A A232C for cell-free expressionBBa_25W5CS8ECoding
Circularly permuted Bacillus megaterium tyrosinase (cp102BmTyr) for cell-free expressionBBa_25MCLLGQCoding
N-terminal domain of split Bacillus megaterium tyrosinase (85) for cell-free expressionBBa_256QZ9BFCoding
C-terminal domain of split Bacillus megaterium tyrosinase (85) for cell-free expressionBBa_25LJJX6RCoding
N-terminal domain of split Bacillus megaterium tyrosinase (157) for cell-free expressionBBa_25JS0JTQCoding
C-terminal domain of split Bacillus megaterium tyrosinase (157) for cell-free expressionBBa_25ASDORDCoding
N-terminal domain of split Bacillus megaterium tyrosinase (201) for cell-free expressionBBa_25ZULNGECoding
C-terminal domain of split Bacillus megaterium tyrosinase (201) for cell-free expressionBBa_25KWZ7YCoding
Hahella sp. CCB-MM4 tyrosinase (HcTyr) Δ(297-335)::GENLYFQMG for cell-free expressionBBa_25X679H9Coding
Hahella sp. CCB-MM4 tyrosinase (HcTyr) Δ(297-466)::GENLYFQ for cell-free expressionBBa_25XWEQZCoding
Laceyella sacchari tyrosinase (LsTyr) for cell-free expressionBBa_25FMI2J3Coding
Streptomyces kathirae SC-1 tyrosinase caddie protein (MelC1) Δ(1–32) for cell-free expressionBBa_25G1BELFCoding
Streptomyces kathirae SC-1 tyrosinase (MelC2) for cell-free expressionBBa_259QMRS2Coding
VsTyr tyrosinase Δ(1–36) Δ(358-370)::GSGSENLYFQMGSGS for cell-free expressionBBa_25OAUNFBCoding
Verrucomicrobium spinosum tyrosinase (VsTyr) Δ(1–36) Δ(358-518)::GSGSENLYFQ for cell-free expressionBBa_25HE674BCoding
Streptomyces avermitilis MA4680 tyrosinase caddie protein (MelC1) Δ(1–26) for cell-free expressionBBa_25S47AAQCoding
S. avermitilis MA4680 tyrosinase caddie protein (MelC1) Δ(1–26) Y92F for cell-free expressionBBa_25UJS5D8Coding
Streptomyces avermitilis MA4680 tyrosinase (MelC2) for cell-free expressionBBa_25AT5O4DCoding
Streptomyces avermitilis MA4680 tyrosinase (MelC2) I42Y for cell-free expressionBBa_25UHOFGECoding

Bacterial Expression of Tyrosinases

NameRegistry LinkType
Bacillus megaterium tyrosinase with Twin-Strep tag under T7 promoterBBa_25O8A1CICoding
Bacillus megaterium tyrosinase E195S A221V with Twin-Strep tag under T7 promoterBBa_25W2IDEFCoding
Bacillus megaterium tyrosinase R209S M215E V218M with Twin-Strep tag under T7 promoterBBa_257XEKG5Coding
Bacillus megaterium tyrosinase G43R M61H F197W Q214D V217A A232C with Twin-Strep under T7 promoterBBa_25F1666HCoding
Circularly permuted Bacillus megaterium tyrosinase (cp102BmTyr) with Twin-Strep under T7 promoterBBa_25N7PNPECoding
N-terminal domain of split Bacillus megaterium tyrosinase (85) with P4, Twin-Strep under T7 promoterBBa_25BEF3HACoding
N-terminal domain of split Bacillus megaterium tyrosinase (85) with Twin-Strep under T7 promoterBBa_25QHCX3ACoding
C-terminal domain of split Bacillus megaterium tyrosinase (85) with P3, Twin-Strep under T7 promoterBBa_252P2FHZCoding
C-terminal domain of split Bacillus megaterium tyrosinase (85) with Twin-Strep under T7 promoterBBa_25S4REWHCoding
N-terminal domain of split BmTyr (157) with P4, Twin-Strep under T7 promoterBBa_25OAHZEKCoding
N-terminal domain of split BmTyr (157) with Twin-Strep under T7 promoterBBa_258A5BGICoding
C-terminal domain of split BmTyr (157) with P3, Twin-Strep under T7 promoterBBa_25PVXVM9Coding
C-terminal domain of split BmTyr (157) with Twin-Strep under T7 promoterBBa_25C7EW3WCoding
N-terminal domain of split BmTyr (201) with P4, Twin-Strep under T7 promoterBBa_255UQOQ3Coding
N-terminal domain of split BmTyr (201) with Twin-Strep under T7 promoterBBa_25Q2A9FNCoding
C-terminal domain of split BmTyr (201) with P3, Twin-Strep under T7 promoterBBa_25JUU582Coding
C-terminal domain of split BmTyr (201) with Twin-Strep under T7 promoterBBa_25VITCSKCoding
HcTyr Δ(297-335)::GENLYFQMG with Twin-Strep tag under T7 promoterBBa_25QZCO7ICoding
HcTyr Δ(297-466)::GENLYFQ with Twin-Strep tag under T7 promoterBBa_25MNPUPUCoding
Laceyella sacchari tyrosinase (LsTyr) with Twin-Strep tag under T7 promoterBBa_25JM1V78Coding
VsTyr Δ(1–36) Δ(358-370)::GSGSENLYFQMGSGS with Twin-Strep tag under T7 promoterBBa_256FZ67WCoding
VsTyr Δ(1–36) Δ(358-518)::GSGSENLYFQ with Twin-Strep tag under T7 promoterBBa_25PCZL6TCoding
Streptomyces avermitilis tyrosinase caddie protein with Twin-Strep tag under T7 promoterBBa_25GXUJ4UCoding
Streptomyces avermitilis tyrosinase caddie protein Y92F with Twin-Strep tag under T7 promoterBBa_25F3Q3UQCoding
Streptomyces kathirae SC-1 tyrosinase caddie protein with Twin-Strep tag under T7 promoterBBa_25F4ETGHCoding
Streptomyces avermitilis MA4680 tyrosinase (MelC2) with Twin-Strep tag under T7 promoterBBa_2526W8NDCoding
Streptomyces avermitilis MA4680 tyrosinase (MelC2) I42Y with Twin-Strep tag under T7 promoterBBa_25PKWE6LCoding
Streptomyces kathirae SC-1 tyrosinase (MelC2) with Twin-Strep tag under T7 promoterBBa_25WEVO4ZCoding

Reporter Gene Expression

NameRegistry LinkType
Tetracycline responsive element-controlled EGFP reporterBBa_25NMLRUMCoding
tTA Induceable EYFP ReporterBBa_K4497028Coding

Kill Switch

NameRegistry LinkType
iCasp9 killswitchBBa_2568YYAPCoding

Backbones

NameRegistry LinkType
pcDNAT7BBa_25GU3656Plasmid_Backbone
pcDNA3.1 based backboneBBa_258AF99IPlasmid_Backbone
pBudCE4.1 Mammalian Expression VectorBBa_25THPLYGPlasmid_Backbone

Primers

NameSequence
1021GGTACCAGGCCACCATGTGCAGAG
1022AAGCTTGATTATTAATTCATGAGCTGTGTAGC
1023GAATTAATAATCAAGCTTCCGCTGAG
1024ATGGTGGCCTGGTACCCTATAGTGAGTC
1031GTACCAGGCCACCATGTACCCGTAC
1032GCGGAAGCTTGATTATTAATTCATCAGTTGGGTGGC
1033TAATCAAGCTTCCGCTGAGCAATAACTAGC
1034ATGGTGGCCTGGTACCCTATAGTGAGTCGTATTATAGAGTCCGGAGGC
142_F_5’LETGGTAGTATCGAGGGTAGAATG
143_R_3’LETGAGGTTATGTTATCGAGCGAG
144_F_3’LETCTCGCTCGATAACATAACCTC
145_R_5’LETCATTCTACCCTCGATACTACC
146_F_gBlockCTTCACCGAGATGACTTAGG
147_R_gBlockGCTATCCGTTCTATCACTGG
148_F_gBlock-SacIATGTAGTACCTTAGTCGAGCTC
149_R_gBlock-NcoICCTTAATCACTCACTTCCATGG
212_R_NTEVp-Stop-NcoI_T7ttCGGTCCATGGTTATTAAGTCTGGAAGTTAG
213_F_NcoI-T7tt_NTEVp-StopAGACTTAATAACCATGGACCGCTGAG
221_F_NTEVp(E75S)ACTGATTGACGGCCGCG
222_R_NTEVp(E75S)GACTGTTGAAGAGTGGTTGTGTTC
231_F_Stop-NcoI-T7ttTAATAACCATGGACCGCTG
232_R_CTEVpATTCATCAGTTGGGTGGC
241_F_BFPATGGTGTCTAAAGGAGAGGAAC
242_R_s(GS)LGCCTCCACAGCCTCC
251_F_SacI-Kozak-CD4SP_T7pTATAGGGAGCTCGCCACCATGTG
252_R_BFP-Stop-NcoI_T7ttGTCCATGGTTATTAGTTCAGCTTATG
253_F_NcoI-T7tt_BFP-StopTGAACTAATAACCATGGACCGCTGAG
254_R_T7p-SacI_Kozak-CD4SPGGTGGCGAGCTCCCTATAGTGAGTC
271_F_tTA-(G4S)2LCGGTGGCGGTGGCAG
272_R_TetRGAGCCAGACTCACACTTCAG
281_F_SacI-RS20_T7pTATAGGGAGCTCGCCACCATG
282_R_CaM-NcoI_pcDNAAGCGGTCCATGGTTATTACTTGGCG
283_F_NcoI-pcDNA_CaMAGTAATAACCATGGACCGCTGAG
292_R_CTEVp-Stop-NcoI_T7ttCAGCGGTCCATGGTTATTAATTCATCAGTTG
293_F_NcoI-T7tt_CTEVp-StopTTAATAACCATGGACCGCTGAG
312_R_P4-Stop-NcoI_T7ttCAGCGGTCCATGGTTATTACCCATACTC
313_F_NcoI-T7tt_P4-StopGTAATAACCATGGACCGCTGAG
321_F_T7tt_HindIIICTTCCGCTGAGCAATAACTAGC
322_R_T7p_KpnICTTGACTGGTACCCTATAGTGAGTCGTATTATAGAG
323_R_backbone_MluI-tTAreGAACGCGTATATCTGGCCCG
324_R_T7p_KpnI2TGGCGGTACCCTATAGTGAGTCGTATTATAGAG
381_F_3’LET_P4TCTGAAGCAGGAAAACCAACAGTTGGAGGAAGAGAACGCAGCCCTGGAGTATGGGTAATAACTCGCTCGATAACATAACCTC
382_R_NBmTyr(85)_P4GTTGGTTTTCCTGCTTCAGAGCCTGGATCTTTTGCTTGAGTTGTGCAATCTTGGAACCGCCAGGGTTAATGCTTTGCAGATC
391_F_CBmTyr(85)_P3GCAGAAGAACGCAGCCCTGAAGGAAAAGAACCAGGCACTGAAGTACGGCGGTTCCGAAGTCACCCTCCCTTACTG
392_R_5’LET_P3TCAGGGCTGCGTTCTTCTGCTCCAGTTGGGCGATCTCTTCCTCAAGCTGTTGGATCATTCTACCCTCGATACTACC
402_R_NBmTyr(157)_P4GTTGGTTTTCCTGCTTCAGAGCCTGGATCTTTTGCTTGAGTTGTGCAATCTTGGAACCGCCCTTAGTCGCACCGAAGTTTC
411_F_CBmTyr(157)_P3GCAGAAGAACGCAGCCCTGAAGGAAAAGAACCAGGCACTGAAGTACGGCGGTTCCGAAGCCCCTACACTTCC
422_R_NBmTyr(201)_P4GTTGGTTTTCCTGCTTCAGAGCCTGGATCTTTTGCTTGAGTTGTGCAATCTTGGAACCGCCGGGACCGTTGATGAAGC
431_F_CBmTyr(201)_P3GCAGAAGAACGCAGCCCTGAAGGAAAAGAACCAGGCACTGAAGTACGGCGGTTCCCAACTGCATAACCGCGTC
452_R_HcTyr1(1-296)-TEVCS(M)_3’LETGAGGTTATGTTATCGAGCGAGTTATTACTGGAAGTACAGGTTTTCG
502_R_VsTyr(37-357)-TEVCS(M)_3’LETGAGGTTATGTTATCGAGCGAGTTATTACTGGAAGTACAGGTTTTCAGAG
571_F_HA_KozakGCCGCCACCATGTACCCGTACGATGTCCC
572_R_TEVp-Stop_pAAVS1CGTTTATTAATTCATCAGTTGGGTGG
573_F_pAAVS1_TEVp-StopAACTGATGAATTAATAAACGCGTTGTACACCCC
574_R_Kozak_HATACGGGTACATGGTGGCGGCTTAATTAAG
631_F_KpnI-Kozak_T7pCTATAGTGAGTCGTATTATAGGTACCGCCACCATG
632_R_Casp9_T2ACTACATCTCCACAAGTTAGTAATGAACCTCTACCCTCTGATGCGCTAGTCTTAAAGAAC
633_F_GFP_T2AACTAACTTGTGGAGATGTAGAAGAGAACCCAGGACCCAGCAAAGGCGAAGAATTG
634_R_GFP-2xStop-HindIIIAAGCTTATTACTTGTACAGCTCG
635_F_T7tt_GFP-2xStop-HindIIIGCTGTACAAGTAATAAGCTTCCGCTGAGCAATAACTAGC
636_R_T7pCTATAGTGAGTCGTATTATAGAGTCC
641_F_EGFP_pcDNACTCACTATAGGGTACCGCCACCATG
641_F_MluI-tTAre_backboneCCAGATATACGCGTTCCCTATCAG
642_R_EGFP_pcDNAATTGCTCAGCGGAAGCTTATTACTTGTACA
642_R_EGFP-Stop-HindIII_T7ttATTGCTCAGCGGAAGCTTATTACTTGTA
651_F_Kozak-CD4SP_T7p-KpnITAGGGTACCGCCACCAT
652_R_BFP-Stop_HindIIIGGAAGCTTATTAGTTGAGCTTATGG
661_F_pET_Twin-StrepCAGTTCGAGAAATAATAAGCGGCCGCACTCG
662_R_TwinStrep_pETGCTTATTATTTCTCGAACTGCGGGTG
663_F_Twin-STrep_pETGAAATACTAGATGTCGGGAAGCGCATG
664_R_pET_Twin-StrepTTCCCGACATCTAGTATTTCTCCTCTTTAATC
671_F_BmTyr_pETAAGAGGAGAAATACTAGATGAGCAACAAGTATAGGGTG
672_R_BmTyr_TwinStrepTGACTCCATGCGCTTCCCGAGGAGGACCGCTTGCTC
673_F_Twin-StrepTCGGGAAGCGCATGGAG
674_R_pETCATCTAGTATTTCTCCTCTTTAATCTC
711_F_BmTyr_pETAAGAGGAGAAATACTAGATGCAACTGCATAACCGC
712_R_BmTyr_TwinStrepTGACTCCATGCGCTTCCCGAGGGACCGTTGATGAAGC
722_R_BmTyr_TwinStrepTGACTCCATGCGCTTCCCGAAGGGTTAATGCTTTGCAG
723_F_TwinStrep_P4TCTGAAGCAGGAAAACCAACAGTTGGAGGAAGAGAACGCAGCCCTGGAGTATGGGTCGGGAAGCGCATGG
732_F_CBmTyr(85)_pETAAGAGGAGAAATACTAGATGGAAGTCACCCTCCCTTAC
734_R_pET_P3TCAGGGCTGCGTTCTTCTGCTCCAGTTGGGCGATCTCTTCCTCAAGCTGTTGGATCATCTAGTATTTCTCCTCTTTAATCTC
742_R_NBmTyr(157)_TwinStrepTGACTCCATGCGCTTCCCGACTTAGTCGCACCGAAGTTTC
752_F_CBmTyr(157)AAGAGGAGAAATACTAGATGGAAGCCCCTACACTTCC
762_R_NBmTyr(201)_TwinStrepTGACTCCATGCGCTTCCCGAGGGACCGTTGATGAAGC
772_F_BmTyr_pETAAGAGGAGAAATACTAGATGCAACTGCATAACCGCGTC
791_F_HcTyr 1 Δ(297-335)::GENLYFQMG_pETAAGAGGAGAAATACTAGATGAAAACCCGGCAGAACG
792_R_HcTyr 1 Δ(297-335)::GENLYFQMG_TwinStrep(726A>T)TGACTCCATGCGCTTCCAGAATCTATCTCGGAATGCAGGG
793_F_TwinStrep(726A>T)_pETTCTGGAAGCGCATGGAG
811_R_HcTyr1(1-296)-TEVCS(M)_Twin-StrepTGACTCCATGCGCTACCCGACTGGAAGTACAGGTTTTCGC
813_F_TwinStrep(281A>T)_pETTCGGGTAGCGCATGG
821_F_LsTyr_pETAAGAGGAGAAATACTAGATGACCACTATCCGCAAG
822_R_LsTyr_TwinStrepTGACTCCATGCGCTTCCCGACTGGTAACGATAACCCAAC
831_F_SavMelC1(27-118)_pETAAGAGGAGAAATACTAGATGGCTCCTTCTGCTTC
832_R_SavMelC1(27-118)_TwinStrepTGACTCCATGCGCTTCCCGAGTTAAATGGGACCAGTGG
841_F_SavMelC2_pETAAGAGGAGAAATACTAGATGACTGTACGCAAGAACC
842_R_SavMelC2_TwinStrepTGACTCCATGCGCTTCCCGAGACTGTGTCGAAGGTGTAATG
871_F_SkMelC1(33-124)_pETAAGAGGAGAAATACTAGATGGCAGGACACCAGC
872_R_SkMelC1(33-124)_TwinStrepTGACTCCATGCGCTTCCCGAGTTCGCAGGAAATGGCAG
881_F_SkMelC2_pETAAGAGGAGAAATACTAGATGACTGTGCGCAAGAAC
882_R_SkMelC2_TwinStrepTGACTCCATGCGCTTCCCGACACGTCGAAGGTATAAAACTTG
891_F_VsTyr(37-357)-TEVCS(M)-VsTyr(371-518)_pETAAGAGGAGAAATACTAGATGAAATACCACAGGCTGAATC
892_R_VsTyr(37-357)-TEVCS(M)-VsTyr(371-518)_TwinStrepTGACTCCATGCGCTTCCCGAGAAAGTGCCGACGGAAAT
901_R_VsTyr(37-357)-TEVCS(M)_TwinStrep(281A>T)TGACTCCATGCGCTACCCGACTGGAAGTACAGGTTTTCAGAG
911_F_MxEnc_LinkerGGATCTGGTGGTGGACCTGACTTCCTGGG
911_F_TEV_LinkerATCTGGTGGTGGGGGCTCCGAAAACCTG
912_R_pcDNA_TyrBmACTTGTTGCTCATGGTGGCGGTACC
913_F_TyrBm_pcDNACGCCACCATGAGCAACAAGTACAGAGTGC
914_R_TyrBm_LinkerTCCACCACCAGATCCGCTGCTCCGCTTGC
921_F_QtEnc_LinkerGGATCTGGTGGTGGAAATAAGTCACAGTTGTATCC
931_F_TmEnc_LinkerGGATCTGGTGGTGGAGAGTTCCTGAAGCGC
941_F_pcDNA_DD-CAGAACTGGAATAATAAAGTCAAGCTTCCGC
942_R_pcDNA_LinkerCCAGAACCCATGGTGGCGGTACC
943_F_Linker_pcDNACGCCACCATGGGCGGCGGAGGATC
943_F_TyrBm_LinkerCACCATGGGTTCTGGTGGAGGCGGAAGTAGC
944_R_DD-C_pcDNAGACTTTATTATTCCAGTTCTAGAAGCTCC
951_F_Linker_MxSigCGTGGGCAGCTTGAGGCGCGGTTCTGGTGGAGGC
952_R_pcDNA_MxSigCTCAAGCTGCCCACGGTAAGCATGGTGGCGGTACC
961_F_Linker_QtSigACTGTTGGUTCTCTGATTCAGGGTTCTGGTGGAGGCG
962_R_pcDNA_QtSigCAGAGAACCAACAGTAAATCCTTTCATGGTGGCGGTACCC
971_F_Linker_TmSigTCTGGGCATTCGGAAGCTGGGTTCTGGTGGAGG
972_R_pcDNA_TmSigTTCCGAATGCCCAGATCCCCCCCCATGGTGGCGGTAC
981_F_MxEncCCTGACTTCCTGGGC
982_R_pcDNACATGGTGGCGGTACC
991_F_QtEncAATAAGTCACAGTTGTATCCG
1001_F_TmEncGAGTTCCTGAAGCGCT
1011_F_Kozak_mCMVAAGCAGAGCTGGTACCGCCACCATG
1011_F_TRE_pcDNAT7v2TACGCATAGGTCGAGTTTACCACTCC
1012_R_mCMV_KozakGTACCAGCTCTGCTTATATAGGCCTCC
1013_F_Kozak_mCMVAAGCAGAGCTGGTACCGCCAC
1013_F_TRE_pcDNAT7v2TACGCATAGGTCGAGTTTACCACTCC
1014_R_pcDNAT7v2_TRECTCGACCTATGCGTATATCTGGCCC
CAG fwdCTCTGCTAACCATGTTCATG
EMCV IRESTTATTCCAAGCGGCTTC
mTagBFPAGTTCCTCTCCTTTAGACACC
WPREATCCAGAGGTTGATTGTCG
Escherichia Coli Human Embryonic Kidney Cells Modular Extracellular Sensory Architecture Tobacco Etch Virus Technical University of Munich Ludwig-Maximilians-Universität München Förster Resonance Energy Transfer Ligand Binding Domain Transmembrane Domain Intracellular Effector Domain Generalized Extracellular Molecule Sensor Synthetic Intermembrane Proteolysis Receptors Transcription Factor Human Embryonic Kidney Human Embryonic Kidney Amino Acid Triangulation Number C-terminal Domain N-terminal Domain Split Protease-Cleavable Orthogonal Coiled-Coil High-Performance Liquid Chromatography Heterodimeric Coiled-Coiled Peptides enhanced Unagi (eel) Green fluorescent protein tetracycline-controlled transactivator Heterodimeric Coiled-Coil Peptide P3 Heterodimeric Coiled-Coil Peptide P4 circular permutation Bicinchoninic acid Bovine Serum Albumin Erythropoietin