R-DETOX 2.0

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RDX 2D

Our Challenge

RDX 3D

RDX (Hexahydro-1,3,5-trinitro-1,3,5-triazine) is a widely used explosive in military munitions and the defense industry. Its production and use have led to the contamination of soils, groundwater, and lakes, as RDX is chemically stable and difficult to degrade. Prolonged exposure to this compound has been associated with adverse health effects, including toxicity to organs such as the liver and kidneys, and the Environmental Protection Agency (EPA) identifies it as a potential carcinogen. Due to its persistence in the environment and associated health risks, there is an urgent need to develop effective, sustainable, and low-cost remediation strategies to remove RDX from contaminated ecosystems.

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Dedicated to saving lives and nature.

0
of the 1699 sites on the National Priorities List (NPL) have RDX contamination.
[1]
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countries have documented RDX contamination.
[2]
+0
military items contain RDX according to the U.S. Department of Defense (DoD).
[3]

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Over the Years

As part of our journey, our team’s initial cycle developed the first prototype of our solution. Since then, subsequent cycles have continuously optimized and refined this design, reflecting the evolution of our prototypes over the years.

RUM-UPRM | 2020
Device Right
Bacteria
RUM-UPRM | 2022
In 2020, our team designed a three-part circuit for RDX, enabling its detection, degradation, and the subsequent lysis of the bacteria. They also developed a separate three-device circuit for mercury bioremediation, which included detection, detoxification, and a blue-light–activated lysis system for biocontainment.
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Bacteria
RUM-UPRM | 2021
Device Right
RUM-UPRM | 2021
In 2021, our team proposed a concept to contain the bacteria using the three-part genetic circuit in a stirred-batch bioreactor system.
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RUM-UPRM | 2022
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Bacteria
RUM-UPRM | 2022
In 2022, our team optimized the RDX genetic circuit by introducing the Hmp/Hcp fusion promoter to address the lack of regulation in the algD promoter.
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Bacteria
RUM-UPRM | 2025
Device Right
RUM-UPRM | 2025
In 2025, our team focused on optimizing Device 2 by introducing a constitutive promoter and adding a riboswitch for tighter regulation. We maintained the key biodegradation genes xplA and xplB, simplified the circuit by removing the remaining components of Device 1, and preserved the killswitch for biosafety. Additionally, we established operational parameters for the Serial Bioreactor System (SeBiS) and incorporated a downstream electrochemical reactor to remove byproducts, ensuring safe and efficient RDX degradation.
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THIS IS OUR PROJECT


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Description

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Education

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Engineering

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Human Practices

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Hardware

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References

[1] Agency for Toxic Substances and Disease Registry (2012, Sep.). Toxicological Profile for RDX [Online]. Available: National Priorities List (NPL)

[2] Cary, T. J., Rylott, E. L., Zhang, L., Routsong, R. M., Palazzo, A. J., Strand, S. E., & Bruce, N. C. (2021, May 3). Field trial demonstrating phytoremediation of the military explosive RDX by XPLA/XPLB-expressing switchgrass. Nature News. Global Map

[3] U.S. Environmental Protection Agency, Federal Facilities Restoration and Reuse Office (2014, Jan.). Technical Fact Sheet – Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) [Online]. Available: EPA RDX Fact Sheet

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