▼ PARTS ▼

BASIC PARTS

Due to codon optimization, there were multiple Basic Parts which function the same but differed from each other in DNA sequence, so the Basic Parts were displayed in different versions.

Part ID Part Name Part Type Part Description
BBa_25OAK6TB HotPETase Coding Encodes HotPETase under the control of a T7 lac promoter. Cells express and secrete the enzyme upon IPTG induction.
BBa_25FWBRO7 HotPETase_core_v1 Coding Encodes HotPETase, codon-optimized as the core module for the CsgA-HotPETase-BaCBM2 fusion protein.
BBa_25KXWHXK HotPETase_core_v2 Coding Encodes HotPETase, codon-optimized as the core module for the CsgA-HotPETase fusion protein.
BBa_25QC4472 HotPETase_core_v3 Coding Encodes HotPETase, codon-optimized as the core module for the HotPETase-BaCBM2 fusion protein.
BBa_25HOIJ11 CsgA_opt_v1 Coding Encodes CsgA, codon optimised for CsgA-HotPETase-BaCBM2 fusion protein.
BBa_259DU4FX CsgA_opt_v2 Coding Encodes CsgA, codon optimised for CsgA-HotPETase fusion protein.
BBa_25322080 BaCBM2_opt_v1 Coding Encodes BaCBM2, codon-optimized for the CsgA-HotPETase-BaCBM2 fusion protein.
BBa_25SA07GK BaCBM2_opt_v2 Coding Encodes BaCBM2, codon-optimized for the HotPETase-BaCBM2 fusion protein.
BBa_25ZERWUB (G4S)2_linker_CsgA-HotPETase_v1 Coding Encodes a flexible linker (G4S)2 for fusion between CsgA and HotPETase, and is codon optimised for CsgA-HotPETase-BaCBM2 trifunctional fusion.
BBa_25DFWCJ3 (G4S)2_linker_CsgA-HotPETase_v2 Coding Encodes a flexible linker (G4S)2 for fusion between CsgA and HotPETase, and is codon optimised for CsgA-HotPETase bifunctional fusion.
BBa_25WIHZ1B (G4S)2_linker_HotPETase-BaCBM2_v1 Coding Encodes a flexible linker (G4S)2 for fusion between HotPETase and BaCBM2, and is codon optimised for CsgA-HotPETase-BaCBM2 trifunctional fusion.
BBa_25FM3WAD (G4S)2_linker_HotPETase-BaCBM2_v2 Coding Encodes a flexible linker (G4S)2 for fusion between HotPETase and BaCBM2, and is codon optimised for HotPETase-BaCBM2 bifunctional fusion.

COMPOSITE PARTS

Composite Parts were engineered sequentially with the three Basic Parts, namely CsgA, HotPETase, and BaCBM2. (G4S)2 linker was used to connect the Basic Parts.

Part ID Part Name Part Type Part Description
BBa_251QH76Y CsgA-HotPETase-BaCBM2 Coding Encodes a trifunctional fusion protein for targeted PET bioremediation.
BBa_252IGL39 CsgA-HotPETase Coding Encodes CsgA-(G4S)2-Hotpetase, a surface display system for whole-cell biocatalysis of PET.
BBa_25IHX9R7 HotPETase-BaCBM2 Coding Encodes a bifunctional enzyme for immobilized PET degradation.