Today, with the global issue of population aging becoming increasingly severe, people are paying more attention to anti-aging–related problems. How to effectively address a series of health issues during the human aging process has become a hot topic of discussion. As age increases, these problems seriously affect people’s quality of life and health span.

At present, the anti-aging products on the market face many dilemmas:Traditional chemical synthesis of NMN is costly, leading to high product prices；Most oral products lack scientific combination formulas, with limited absorption and utilization；Skincare-related antioxidant products mostly rely on a single ingredient, with insignificant effects, and cannot balance internal metabolic regulation with external skin repair；More importantly, the issue of premature aging in men caused by metabolic diseases such as diabetes and obesity has not yet been sufficiently addressed.

Therefore, our team plans to launch a set of synthetic-biology–based dual-version anti-aging product series—ChronoCure:

Female version: focusing on oral NMN + glutathione (GSH) solution and external-use facial mask, combining internal antioxidant effects with external skin repair.

Male version: designed as NMN + GLP-1 capsules, targeting the premature aging pain points of people with diabetes and obesity, in order to delay the aging process.

Our product series is based on cutting-edge research in synthetic biology and biomedicine, providing comprehensive anti-aging solutions for both women and men. By combining Nicotinamide Mononucleotide (NMN) with multiple synergistic active substances, our formula offers scientific synergy in cellular energy metabolism, oxidative stress resistance, and metabolic regulation.

Female Oral Solution

NMN directly increases intracellular NAD⁺ levels, enhances mitochondrial function, and delays cellular senescence. Glutathione (GSH), as a core antioxidant, eliminates free radicals and reduces oxidative damage. The synergy of NMN + GSH creates dual protection, acting from both metabolic and antioxidant dimensions, providing women with a comprehensive internal anti-aging solution.

Female Skincare Product

NMN and GSH are applied through silk sheet masks and hyaluronic acid essence. NMN promotes dermal cell energy renewal. GSH resists oxidative stress from UV rays and pollution. Hyaluronic acid provides deep hydration and barrier repair. This triple-function design achieves antioxidant protection, cellular repair, and visible skin revitalization.

Male Capsule

Figure 1: Product Concept Diagram

NMN restores intracellular NAD⁺ levels, while GLP-1 (glucagon-like peptide-1), a recombinant peptide closely related to glucose and lipid metabolism, can alleviate accelerated aging caused by obesity. This combination regulates blood glucose, improves insulin sensitivity, and reduces systemic inflammation, thereby providing men with targeted metabolic anti-aging benefits.

We define potential customers as the target groups likely to purchase and use ChronoCure products, while stakeholders refer to individuals or institutions that play a key role in the development of ChronoCure. Through analysis and interviews (see iHP section), we further clarified the characteristics and needs of the core customer groups and summarized them into the following three categories:

1、Middle-aged executive group This group is the most core potential customer segment of ChronoCure. They generally focus on how to balance life and health amid busy careers and family responsibilities, and regard anti-aging as a form of “health investment.” They usually have strong purchasing power, with high demands for quality and brand endorsement. They rarely remain at the level of “basic life security” but instead pay more attention to scientific evidence, long-term value, and identity recognition. Therefore, their acceptance of ChronoCure depends not only on its efficacy but also on brand positioning and user experience.

2、Private medical aesthetics institutions These are not only important channel partners but can also strengthen ChronoCure’s market influence through clinical application and client recommendations. By integrating ChronoCure products into their service system, medical aesthetics institutions can provide clients with an integrated “medical aesthetics + nutrition” anti-aging solution.

3、Nutritional health product companies As strategic partners, these companies can form complementary advantages in production, supply chain, and market expansion. At the same time, they may also become potential competitors, driving ChronoCure to maintain competitive barriers in product differentiation, brand positioning, and scientific research strength.

By clarifying the roles of different customers and stakeholders, we can more effectively optimize product design, market strategies, and cooperation models, laying a solid foundation for the sustainable development of ChronoCure.

Middle-aged executive group

Figure 2: Parent Interviews

Our parents are mostly executives, with ages concentrated between 45–50. Through interviews with them, we found that they generally find it difficult to balance heavy professional responsibilities, personal health, and family life, and that long-term stress, irregular routines, and metabolic problems accelerate aging. Their common pain points include declining energy, slowed metabolism, and early skin aging. Therefore, women tend to prefer oral solutions and facial masks, while men lean toward convenient and efficient capsule dosage forms. This group generally places great importance on long-term health maintenance and is willing to invest in anti-aging solutions with scientific evidence. Based on their dual pursuit of efficacy and brand trust, we position middle-aged executives as the core potential customer group of ChronoCure. They are not only expected to be early adopters of the product but may also promote its widespread dissemination through their own influence.

Health Investment

The executive group regards anti-aging products as a long-term investment in health and quality of life, rather than just daily supplements. They pay less attention to basic survival needs, instead pursuing solutions that can enhance vitality, improve appearance, and extend healthy lifespan.

Science and Safety

They emphasize that product use must be based on scientific validation and safety assurance. They express concerns about the authenticity of some NMN products on the market and show a stronger preference for products with transparent ingredients, verified efficacy, and compliance with international standards.

Convenience and Lifestyle Fit

Given their busy schedules, the executive group prefers convenient and efficient anti-aging solutions that easily integrate into daily life. Ready-to-drink NMN+GSH oral solutions, as well as simple facial masks and serums, are considered highly attractive.

Brand and Identity Recognition

The executive group favors brands that reflect exclusivity and status. They hope that ChronoCure will not only deliver health benefits but also provide a sense of exclusivity and prestige, thereby reinforcing their identity as pioneers in health investment.

Gender Differences

Female executives pay more attention to appearance and skin condition, preferring solutions that combine nutrition with medical aesthetics and skincare. Male executives, on the other hand, tend to focus more on nutritional supplementation and fitness to maintain health, favoring products such as NMN+GLP-1 capsules that act directly on metabolism and energy.

Customer journey

Figure 3: Customer journey

In a business plan, user needs analysis requires not only understanding customer pain points and preferences, but also gaining deep insight into the entire process of contact, purchase, usage, and feedback—the so-called user journey. By mapping the user journey, we can identify key touchpoints, optimize product design and service models, and thereby enhance user experience and loyalty. For ChronoCure, middle-aged executives are the most core and strategically significant potential customers. Their characteristics in health investment, brand identity, and long-term anti-aging needs make their user journey analysis especially crucial. The user journey also helps us in future customer interactions.

Private medical aesthetics institutions

Figure 4: Medical Aesthetic Institutions

Through interviews with private medical aesthetic institutions, we found that they are highly focused on the upgraded demand of clients for comprehensive anti-aging solutions, not only hoping to improve appearance and skin condition, but also expecting to achieve deeper health benefits, in order to align with the concept of “Beauty + Health.”

As an innovative product based on synthetic biology and biomedical research, ChronoCure can provide an integrated solution that combines oral supplementation (such as NMN capsules or liquids) with topical care (such as masks and serums), meeting the institutions’ need to create holistic service packages that enhance customer satisfaction and perceived value.

At the same time, institutions pointed out that endorsement from scientific research organizations can make ChronoCure more convincing, effectively addressing clients’ concerns about safety and efficacy.

They also emphasized that personalized experiences and a sense of exclusivity are crucial factors in attracting high-end customers, and ChronoCure’s customization potential and exclusive positioning precisely help them achieve brand differentiation.

Private medical aesthetic institutions believe that we can become strategic partners, hoping to jointly expand the market and strengthen client loyalty through co-branded campaigns or bundled service packages.

Nutritional Health Product Enterprises

Figure 5: Screenshot of Tencent Meeting with the Nutritional Health Product Enterprise Owner

Through our interview with the owner of a nutritional health product enterprise, we found that she pays close attention to the market potential of NMN and related anti-aging products, while also pointing out that the current production and commercialization models face significant obstacles. She noted that existing NMN products generally suffer from high synthesis costs, complex purification processes, and unstable supply chains. For this reason, she expressed strong interest in ChronoCure’s synthetic biology approach, believing it has the potential to significantly reduce costs, enhance large-scale production capacity, and improve product accessibility. At the same time, she emphasized that in order to gain a foothold in the global market, ChronoCure must be well-prepared with comprehensive clinical data, safety documentation, and compliance strategies to meet international regulatory standards. She further stated that if ChronoCure’s formulations demonstrate superior efficacy and differentiation compared to current products, her enterprise would be willing to pursue collaborations such as joint ventures, co-branding, or licensing agreements, considering us a highly promising strategic partner. However, she also acknowledged that ChronoCure may eventually become a direct competitor, particularly once we establish independent production systems and consumer-facing branding. This dual role positions nutritional health product enterprises as both potential collaborators and inevitable competitors.

It is essential to conduct an in-depth analysis of the customer needs that existing anti-aging products have not yet fully addressed. By identifying these unmet demands in the market, ChronoCure can uncover important opportunities that need to be filled.

Factors	Chemically Synthesized NMN Products	Single Glutathione Products	Ordinary Skincare Products	ChronoCure Women’s Oral Liquid	ChronoCure Women’s Skincare	ChronoCure Men’s Capsules
Scientific validation	🔴 Partially lacking	🟡 Limited clinical evidence	🟡 Has foundation but lacks systematic anti-aging	🟢 Synthetic biology + research support	🟢 Ingredient ratio supported by research	🟢 GLP-1 with mature clinical basis
Safety	🔴 Non-transparent in part	🟡 Safe but unstable dosage	🟡 Relatively safe	🟢 Transparent ingredients, standardized dosage	🟢 Low irritation, suitable for medical aesthetics	🟢 Clinically mature peptides + safe dosage
Bioavailability	🔴 Low absorption rate	🟡 Limited absorption rate	🔴 No oral effect	🟢 Liquid form, improves utilization rate	🟡 —	🟢 Capsule delivery, stable absorption
Antioxidant efficacy	🔴 Limited	🟡 Moderate	🟡 Local effect	🟢 NMN + GSH synergy	🟢 Topical antioxidant + repair	🟢 Assists in reducing inflammation
Metabolic regulation	🟡 Basic	🔴 None	🔴 None	🟡 Secondary	🟡 Secondary	🟢 Directly targets metabolic early-aging pain points
Skin & appearance	🟡 Secondary	🟡 Secondary	🟢 Visible improvement	🟡 Secondary	🟢 Directly targets skin aging	🟡 Secondary
Convenience	🟢 Capsule form relatively convenient	🟡 Powder/tablet	🟢 Easy application	🟢 Ready-to-drink small bottles	🟢 Mask + serum set	🟢 Capsule, portable
Brand credibility	🔴 Chaotic market	🔴 Generally lacking	🟡 Mostly mass-market brands	🟢 Scientific research endorsement	🟢 Medical aesthetics cooperation channels	🟢 Positioned for health investment groups
Affordability	🔴 Relatively expensive	🟡 Moderately priced	🟡 Diverse pricing	🟡 Mid-to-high-end positioning	🟡 Mid-to-high-end positioning	🟡 High-end positioning

Table 1: Unmet Needs of Existing Products (Insufficient → 🔴 Red; Secondary / Limited → 🟡 Yellow; Clear Advantage → 🟢 Green)

Analysis

The following analysis focuses on the unmet needs in the anti-aging market, starting from the perceptions and pain points of target customers, and further examining the product features of ChronoCure to demonstrate how it meets user expectations.

Unmet Needs

Efficacy

Potential customers commonly report that existing anti-aging products (such as single-ingredient NMN supplements or basic skincare items) have limited effects, failing to deliver significant improvements in energy, metabolism, or appearance. Users often complain about slow onset, weak results, and lack of scientific validation.

ChronoCure: The NMN+GSH oral liquid enhances mitochondrial energy and antioxidant protection, the skincare set delivers visible rejuvenation effects, and the NMN+GLP-1 capsules directly address metabolic imbalance, ensuring scientifically supported multidimensional efficacy.

Safety

Consumers are concerned about the lack of transparency in the quality and authenticity of current NMN products, many of which do not disclose ingredient sources or dosage information. Market skincare products may also contain irritating substances or chemicals with potential side effects.

To address these pain points, ChronoCure places safety and transparency at its core: it uses the internationally recognized safe probiotic chassis E. coli Nissle 1917 as the production foundation; in future manufacturing, it will adopt high-standard purification, along with strict sterilization and quality monitoring at every stage to prevent potential risks; all product formulations will remain open and transparent, with clearly labeled ingredient sources and standardized dosages, fully complying with international certifications and regulatory standards.

Absorption Rate

Current NMN capsules often suffer from poor gastrointestinal absorption and rapid degradation, limiting their effectiveness; single antioxidants like glutathione also have low oral bioavailability.

ChronoCure’s oral liquid design significantly improves absorption efficiency, while GLP-1 in the capsules ensures stable metabolic regulation.

Appearance & Skin Improvement

Female executives particularly noted that most anti-aging supplements show no obvious skin improvement, while existing skincare products are often limited to surface hydration or whitening, unable to address cellular oxidative damage.

ChronoCure’s masks and serums, combining NMN, GSH, and hyaluronic acid, achieve deep repair, antioxidant protection, and visible rejuvenation—precisely meeting this unmet need.

Metabolic Regulation

Male executives pointed out that current NMN supplements cannot address accelerated aging issues linked to obesity, diabetes, or metabolic syndrome, and that existing products lack targeted metabolic regulation.

ChronoCure tackles this by combining NMN with GLP-1 in capsules, integrating NAD⁺ restoration with clinically validated metabolic control.

Brand Credibility

Executives emphasized that existing anti-aging products are often over-marketed and under-researched, leading to declining consumer trust.

ChronoCure, leveraging synthetic biology innovation, establishes itself as a scientifically credible and socially recognized solution.

We conducted ChronoCure’s market evaluation by analyzing the Serviceable Obtainable Market (SOM), the Serviceable Available Market (SAM), and the Total Addressable Market (TAM) for NMN-based anti-aging products.

Figure 6: Overall NMN Market

Summary

Through the analysis of SOM, SAM, and TAM for NMN anti-aging products, ChronoCure has identified its entry point in South China, expansion opportunities in the Chinese market, and high growth potential on a global scale. The SOM, SAM, and TAM analysis confirms ChronoCure’s significant potential for long-term profitability.

For ChronoCure, it is crucial to identify potential competitors and closely monitor their market dynamics, product innovations, and marketing strategies. This enables us to adjust our positioning in a timely manner and highlight our competitive advantages.

Direct Competitors

ChronoCure’s direct competitors are NMN products with highly similar mechanisms of action, primarily focused on boosting NAD⁺. These competitors share the same target customer base with us, and their value propositions almost completely overlap—anti-aging, vitality enhancement, and lifespan extension. Representative brands include GeneHarbor, Elysium, and Herbalmax, whose strong market recognition and brand influence make them the most direct rivals of ChronoCure.

Indirect Competitors

ChronoCure’s indirect competitors are products with different mechanisms but the same ultimate goal: anti-aging, vitality enhancement, and health improvement. Examples include collagen, resveratrol products, coenzyme Q10, traditional Chinese tonics, and fitness nutritional supplements. Despite diverse mechanisms, these products compete with NMN for the same consumer budget and attention.

Future Competitors

ChronoCure’s future competitors are emerging anti-aging technologies that are not yet widely commercialized but hold disruptive potential. These include epigenetic reprogramming therapies, senolytic drugs, novel NAD⁺ activation supplements, and AI-based personalized longevity management platforms.

Although still in early stages, such technologies may reshape the entire nutritional supplements and health management market.

Category	Representative Products / Companies	Threats / Opportunities	ChronoCure’s Response Strategies
Direct Competitors	NMN brands such as GeneHarbor, Elysium, Herbalmax	Threats: Same mechanism of action (boosting NAD⁺), highly overlapping target customer groups, strong brand recognition. Opportunities: Market education already completed, consumers have high awareness of NMN products.	Highlight scientific validation (synthetic biology, clinical data). Provide gender-differentiated formulas (female oral + skincare, male metabolic capsules). Launch an integrated solution (oral + topical), creating differentiated competitive barriers.
Indirect Competitors	Collagen supplements, resveratrol, coenzyme Q10, traditional Chinese tonics, fitness nutritional supplements	Threats: Compete for the same health budget and consumer attention, wide price range, easily attract consumers seeking “alternative solutions.” Opportunities: Most lack scientific validation or cannot provide comprehensive anti-aging effects.	Strengthen multi-dimensional efficacy (metabolism + antioxidant + skin repair). Build a high-end brand image, distinguishing from mass-market health supplements. Establish presence in medical aesthetics/medical channels, emphasizing medical-grade scientific support.
Future Competitors	Epigenetic reprogramming, senolytic drugs, novel NAD⁺ activators, AI-based personalized longevity management platforms	Threats: Technologies with disruptive potential may reshape the entire anti-aging supplements and health management market. Opportunities: Still in early stages, requiring clinical and market validation.	Continuously invest in R&D and technology iteration. Build partnerships with research institutions, hospitals, and medical aesthetics platforms. Maintain product upgrade flexibility, ready to incorporate new scientific findings into the product line.

Table 2: Analysis of ChronoCure’s Future Competitors

PESTEL Analysis for NMN Industry

Figure 7: PESTEL Radar Chart of the NMN Industry

Economic scored the highest, indicating that market size and growth potential are the most prominent.

Social, Technological, and Environmental dimensions performed strongly, reflecting robust demand, significant technological drivers, and a clear trend toward green production.

Political and Legal dimensions were relatively weaker, mainly constrained by policy uncertainties and compliance risks.

Strategic Planning

To increase the probability of entrepreneurial success, ChronoCure has formulated multiple strategies to address shortcomings and mitigate potential risks.

Weaknesses and threats	Strategies
Regulatory uncertainties in China	Engaging with legal advisors to ensure compliance with cross-border e-commerce rules Monitoring NHC and FDA policies to quickly adapt
High market competition	Highlighting differentiated value (gender-specific formulas, oral + skincare combination) Building brand credibility through scientific validation
Limited public awareness of NMN safety and efficacy	Launching science-based educational campaigns Collaborating with medical beauty institutions to strengthen customer trust
Potential consumer confusion about multiple product lines	Developing clear product pages with Q&A Hosting online live sessions for customer guidance
Consumer bias toward established international brands	Forging partnerships with renowned healthcare & wellness brands Broadening advertising channels across digital and offline platforms
Risk of technological imitation	Applying for patents on formulations and delivery methods Enhancing product functionalities (e.g., GLP-1 synergy, supramolecular eutectic tech)

Table 3: ChronoCure Strategic Planning

Figure 8: Summary of Product Development Plan Interviews — Liu Chao, Investment Manager at China Merchants Bank 1-1, 2; Director of Corporate Planning at LZU Research Institute (Listed Company) 1-3; YunGu Coffee 2-1, 2; Qianjiang Ventures 2-3

During the second interview with Professor Zhao, Director of Corporate Planning at LZU Research Institute (Listed Company), as well as interviews with the investment manager at China Merchants Bank, Qianjiang Ventures, and high-end brand operation teams, we came to deeply realize that enterprise development requires not only a clear strategic plan and stable financing channels but also the integration of brand positioning, market expansion, and consumer experience. These insights have provided important guidance and direction for ChronoCure in shaping its future development path.

Timeline

Figure 9: Product Development Timeline

Blue (R&D): Product research and development, trial sales, new product pipeline, clinical validation, continuous iteration

Orange (Business): Company registration, financing, market education, large-scale production, digital platform, subscription model

Purple (Internationalization): Overseas expansion, FDA/EMA certification, global anti-aging ecosystem

Milestone —— ChronoCure

Figure 10: ChronoCure Development Milestones

Revenue Sources

At different stages of product development, ChronoCure’s investment priorities and revenue streams vary. Based on the characteristics of each stage, we have planned a practical and feasible revenue curve to ensure the product achieves long-term sustainable development.

Figure 11: ChronoCure Revenue Growth Curve

Production Pathway

Figure 12: Product Production Process

In ChronoCure’s production and distribution process, every stage of product development and manufacturing has been carefully designed and strictly controlled to ensure safety, scientific rigor, and sustainability. The SR-Shenzhen team has planned a complete production pathway—from strain optimization to end-user application—with each step taking into account biosafety, quality control, and user experience.

Advertising

Figure 13: SR-Shenzhen Advertising Promotion Strategies

SR-Shenzhen is committed to promoting our ChronoCure anti-aging products to target user groups through multi-level strategies. Our approach includes experiential marketing, online and offline advertising campaigns, and collaborations with authoritative endorsements to maximize public awareness and brand credibility. Advertising is crucial for making our products widely recognized. We will combine poster placements, social media campaigns, and the influence of health bloggers and beauty KOLs to effectively reach middle-aged executives, female consumers, and young people concerned about their parents’ health.

In addition, partnerships with medical aesthetics institutions, nutrition and healthcare enterprises, and relevant industry associations will help us establish a professional image and strengthen public trust, thereby facilitating market expansion. At the same time, through interviews and feedback from partners, we have also identified current advertising trends to ensure that our promotional strategies remain aligned with the latest market dynamics.

Resources

Figure 14: This diagram summarizes the key partners, key resources, and key activities mentioned above.

Risk Analysis

In advancing SR-Shenzhen’s anti-aging products (NMN, GSH, GLP-1) from the laboratory to the market, we have identified and summarized the major potential risks and proposed corresponding solutions to ensure sustainable development and successful commercialization.

PHASE	RISK	POSSIBLE SOLUTIONS
Regulation	Risk of approval delay or policy changes for NMN in China.	Continuously track policy updates from the National Health Commission and NMPA; maintain communication with legal advisors; initiate sales through cross-border e-commerce while preparing filing materials.
Funding	Large funding required for R&D and industrial scaling; insufficient investment may restrict progress.	Actively connect with venture capital, private banks, and government funds; conduct brand promotion to enhance investment appeal; adopt phased financing or joint R&D if necessary.
Upscaling	During industrial-scale production, fermentation efficiency may decrease or product purity may be insufficient, affecting large-scale supply.	Collaborate with universities and biomanufacturing plants to optimize processes; introduce third-generation engineered strains and advanced purification methods; establish a small pilot–pilot–mass production iterative system.
Production Quality	NMN, GSH, and GLP-1 may show batch-to-batch variation during production, impacting stability and consumer trust.	Introduce GMP-certified factories and quality control systems; involve experienced production experts; establish strict product traceability and testing procedures.
Advertising & Selling	Some consumers may hold biases against “synthetic biology” products or believe NMN and other anti-aging products lack scientific basis.	Conduct science communication campaigns; invite doctors, nutritionists, and researchers for endorsements; use medical aesthetics institutions, health influencers, and middle-aged executive case studies to increase credibility.

Table 4: ChronoCure Risk Analysis

Exit Strategy

Figure 15: Outline of Team Exit Strategies

Based on recommendations from investment managers and entrepreneurship advisors during interviews, SR-Shenzhen has designed feasible exit strategies to minimize potential losses while leaving room for future development:

1、Horizontal Merger

Merging with large nutrition, healthcare, or medical aesthetics groups to leverage their capital, distribution channels, and brand advantages, thereby expanding product market acceptance. By utilizing investment banking and M&A advisory resources, the team can identify potential partners with global sales networks to achieve capital and resource integration.

2、Licensing

Licensing our core strains, production processes, or brand to multinational healthcare or biotechnology companies. Through licensing agreements, we can secure stable royalty income and brand usage fees, while leveraging the partner’s capital and production capacity to accelerate product scaling.

3、Strategic Partnership

Forming long-term strategic alliances with medical aesthetics institutions, pharmaceutical companies, and cross-border e-commerce platforms to share sales channels and R&D resources. This approach not only reduces the risks of independent operations but also enhances brand influence.

Skills

Behind ChronoCure stands a passionate student team from Shenzhen. The members come from diverse academic backgrounds spanning life sciences, computer science, economics, and the humanities. This interdisciplinary nature not only ensures rigorous experimental practices but also brings comprehensive perspectives on social, economic, and ethical considerations. With solid training in molecular cloning, metabolic engineering, and fermentation, the team has successfully constructed engineered strains capable of synthesizing NMN, GSH, and GLP-1, laying a strong technical foundation for a scalable anti-aging solution.

Beyond scientific expertise, SR-Shenzhen members also excel in business planning, market research, and human practices, enabling the integration of scientific breakthroughs with real market needs. Our shared vision is rooted in a deep awareness of the challenges posed by an aging society, aiming to create accessible, sustainable, and science-driven health products.

On Our journey of research and entrepreneurship, the team has received strong support from mentors and experts in synthetic biology, nutrition, and investment management. These mentors have provided crucial guidance in experimental design, biosafety assessment, and commercialization pathways. At the same time, SR-Shenzhen has also benefited from collaboration with academic institutions and private investment advisors, gaining access to advanced experimental platforms and drawing valuable experience in bridging the gap between the laboratory and industrialization.

Capabilities

ChronoCure’s core capability lies in transforming synthetic biology research achievements into scalable anti-aging products in a stable and sustainable manner.

R&D Capabilities

At the research and development level, the team has successfully constructed multiple generations of optimized engineered strains:

NMN: Achieved efficient synthesis through multi-gene module optimization (NiaP, nadV, BaPRS, PnuC).

GSH: Enabled one-step glutathione synthesis by expressing the bifunctional enzyme GshF.

GLP-1: Realized secretory production using the PelB signal peptide, providing feasibility for future oral formulations.

These achievements have been validated under laboratory shake-flask and small-scale fermentation conditions, showing stable yields and good purity.

Production Capabilities

At the industrialization level, the team is capable of translating laboratory results into industrial fermentation and downstream purification. Plans for future production include:

Using fed-batch fermentation to enhance the stable yield of NMN.

Applying batch fermentation + cell disruption + chromatographic purification for GSH and GLP-1 to ensure quality and safety.

Establishing a closed-loop production system for biosafety: sterilizing fermentation residues at 121 °C, using dedicated purification pipelines to avoid leakage, and implementing environmental monitoring to ensure zero external release.

Productization and Market Capabilities

In terms of productization and market transformation, ChronoCure’s strengths are reflected in:

Diverse product formats: Women may choose NMN + GSH oral liquids and skincare products, while men may opt for NMN + GLP-1 capsules.

Supply chain and business model: Future production lines will ensure stable raw materials through fermentation and purification factories, followed by product formulation and packaging in dosage-form plants, and finally distribution via e-commerce platforms, medical aesthetics institutions, and healthcare channels.

This capability system means that ChronoCure can not only solve the technology transfer challenges from laboratory to industry but also establish a closed loop between product development and market delivery. Looking ahead, with factory expansion and international deployment, ChronoCure has the scalable potential to grow from the South China market to nationwide and even global markets.

Stakeholders

To achieve the sustainable development of ChronoCure, SR-Shenzhen recognizes that establishing and maintaining long-term, stable stakeholder relationships is essential. We not only need to consider how to sustain collaboration but also clarify what value we can offer them and what they, in turn, can bring to us.

Accordingly, the team has divided stakeholders into four main categories: Customers, Specialists, Business Partners, and Competitors, and has developed corresponding strategies for interaction and collaboration with each group.

1、Customers

Figure 16: ChronoCure’s Target Customer System

ChronoCure’s target customer system consists of three core groups—middle-aged executives, medical aesthetics institutions, and nutrition & healthcare enterprises. These groups are not only potential users but also play varying roles in shaping the product’s promotion pathways and brand image. In our communications, these customer groups generally expressed strong willingness to collaborate: they hope to become future users of our products while also showing interest in investment and long-term partnerships, thereby laying a solid foundation for ChronoCure’s commercialization and sustainable development.

For more details, please refer to Human Practice

2、Specialists

Figure 17: Summary of ChronoCure’s Expert and Mentor Engagement

The growth of ChronoCure would not have been possible without the support of expert mentors. With guidance from patent agents, investment and financing advisors, Professor Zhao, and university research mentors, we have received systematic instruction in intellectual property protection, financing strategies, market pricing, and scientific optimization. This has enabled our team to understand the key steps for commercial implementation, helping us move from “experimental feasibility” to “industrial feasibility.”

3、Business Partners

Figure 18: Summary of ChronoCure’s Partner Engagement

ChronoCure defines business partners as collaborators who can achieve mutual benefit across different links of the production, sales, and branding chain. Venture capital institutions and biotech companies provide us with financing guidance and pathways for industrialization; high-end brand case studies offer inspiration for brand building; beauty salons and medical aesthetics channels help us access premium consumer markets and achieve brand synergy; and future cooperation with GMP-certified manufacturing enterprises will ensure the safety and quality of large-scale production. In this way, we aim to realize coordinated and mutually beneficial collaboration across the entire chain of capital, branding, channels, and manufacturing.

4、Competitors

In the anti-aging product market, competition is intense: international brands dominate the high-end segment, while domestic manufacturers compete in the mid-range market. To maintain competitiveness, SR-Shenzhen has adopted a dual strategy:

Technological Leadership: Continuously optimizing strains and pathways, exploring the synergistic mechanisms of NMN and GSH, as well as the potential of GLP-1 in metabolic regulation.

Patent Protection: Initiating the application process for core patents to secure technological barriers in strains, fermentation methods, and product formulations.

ID	Category	Patent Type	Patent Content	Description
C1	Product Patent	Invention Patent	NMN + GSH + GLP-1 Synergistic Anti-Aging Formulation	First-of-its-kind multi-pathway synergistic combination targeting metabolism, antioxidation, and endocrine regulation, offering significant differentiation advantages.
C2	Product Patent	Invention / Utility Model	Integrated Oral Liquid and Facial Mask Antioxidant Product	Dual-effect design of “internal regulation + external nourishment,” adaptable to both medical aesthetics and home use scenarios.
C3	Process Patent	Invention Patent	Efficient NMN Biosynthesis Pathway (NiaP + nadV + BaPRS + PnuC)	Multi-gene synergy improves conversion rate and yield, enabling industrial-scale production.
C4	Process Patent	Invention Patent	One-Enzyme GSH Biosynthesis System (GshF Fusion Enzyme)	Single bifunctional enzyme enables one-step glutathione synthesis, reducing cost and by-products.
C5	Process Patent	Invention Patent	GLP-1 Secretion and Purification System (PelB-GLP-1)	Constructed a secretory expression system to improve peptide purity and stability.
C6	Process Patent	Invention / Utility Model	Freeze-Drying and Protective Agent Formulation (Moisture < 5%)	Maintains cell viability and stability, extends shelf life, suitable for home storage at room temperature.
C7	Application Patent	Invention Patent	Women’s Antioxidation + Skin Repair Combined Solution	Jointly applied with medical aesthetics institutions to achieve “post-surgery repair + anti-aging skincare.”
C8	Application Patent	Invention Patent	Precision Nutrition Intervention (Middle-aged / High-stress / Sedentary Populations)	Personalized recommendation algorithm based on lifestyle and metabolic status.
C9	Application Patent	Invention Patent	Dual Suicide System (Cold-Inducible + Arabinose-Inducible)	Enhances biosafety by preventing strain leakage and environmental spread.
C10	Application Patent	Invention Patent	Fermentation Residue and Medium Recycling System	Enables green manufacturing and resource reutilization, aligned with ESG and SDG standards.

Table 5: ChronoCure Future Patent Application List

Figure 19: Positive Impacts in NMN Product Development

The long-term impact of ChronoCure lies not only at the scientific level (safety, efficacy, low side effects), but also at the social level (delaying aging, improving quality of life, reducing chronic disease risks), while carrying significance at the environmental level (green production and sustainable development).

Although we face regulatory, market, and economic risks, through continuous scientific iteration, compliance strategies, and brand building, ChronoCure has the potential to become a global anti-aging solution that balances health, sustainability, and social value.

Risks

Figure 20: Navigating Risks in NMN Product Development

Our project faces several risks: safety risks such as potential strain leakage and public misperceptions of synthetic biology; regulatory risks arising from varying international policies on NMN; market risks due to intense competition and limited differentiation; and economic risks related to high upfront costs and uncertain short-term returns. In the future, we will establish closed-loop production and transparent monitoring, closely track and comply with global regulations, enhance product differentiation and brand credibility, and adopt phased financing with an asset-light production model—ensuring sustainable growth.

ChronoCure’s future development will follow a “research-driven + business transformation” dual-path approach. We will first focus on achieving profitability to ensure a sustainable cash flow foundation for the project. On this basis, the team will continuously optimize the chassis strains and production processes, ensuring low-cost and high-yield raw material supply, thereby forming a solid technological barrier. At the same time, we will design more differentiated product portfolios. Through the coordinated advancement of scientific research and market strategies, ChronoCure will gradually realize the industrial implementation of research achievements and establish a multi-level, diversified anti-aging solution system.

【1】NMN market supervision: Which are the safest and most reliable NMN brands? (2024). NetEase News. Retrieved July 13, 2025, from https://c.m.163.com/news/a/JANI86D105566JGZ.html

【2】China’s new NMN policy dynamics and competitiveness analysis of the world’s top seven anti-aging brands in 2025. (2025). NetEase News. Retrieved July 13, 2025, from http://m.163.com/dy/article/K1PTU9OD0550UDQD.html

【3】[No title]. (2025). Sina Technology. Retrieved July 13, 2025, from http://finance.sina.cn/tech/2025-05-29/detail-ineyfkhe3787376.d.html

【4】YHResearch report: Global NMN industry trends 2025–2031. (n.d.). Gelonghui. Retrieved July 13, 2025, from https://m.gelonghui.com/p/2029890

【5】Decoding rock codes and constructing a tea tree NMN biosynthesis system: An innovative exploration of longevity tea driven by mineral–microbe synergy. (2025). Docin. Retrieved July 13, 2025, from https://m.book118.com/html/2025/0516/6041234243011125.shtm

【6】Tianjin Institute of Industrial Biotechnology, CAS, improves NMN production via de novo biosynthesis pathway. (2024). Tianjin Institute of Industrial Biotechnology, CAS. Retrieved July 13, 2025, from http://www.tib.cas.cn/xwdt/kyjz/202412/t20241224_7509412.html

【7】[No title]. (n.d.). Zhonghongwang. Retrieved July 13, 2025, from https://www.zhonghongwang.com/show-140-401180-1.html

【8】Enzymatic production of NMN. (2024). Shandong Testing Center. Retrieved July 13, 2025, from https://www.sdaps.cn/cgzh/swyw/2024/0111/1689.html

【9】Will NMN become inactive during hot weather transportation? W+ Telomere Tower preservation method and three international brand recommendations. (2025). Phoenix Net Shandong. Retrieved July 13, 2025, from https://sd.ifeng.com/c/8kqRbtG2l0I

【10】Cross-border e-commerce chaos needs resolution: Industry calls for active NMN supervision. (2024). China Internet Finance. Retrieved July 13, 2025, from https://finance.china.com.cn/roll/20240719/6143145.shtml

【11】Hu, L. (2021). “Miracle anti-aging drug” NMN: How can it be effective if it is not legal? China Economic Net. Retrieved July 13, 2025, from http://www.ce.cn/cysc/yy/hydt/202102/01/t20210201_36279299.shtml

【12】[No title]. (n.d.). Bontac Bio. Retrieved July 13, 2025, from https://www.bontac-bio.com/en/newsinfo4.html