New Basic Parts

Name	ID	Type	Length(bp)	Function
ELP	BBa_25SG7TTA	Coding	612	An elastin-like peptide is encoded for temperature-responsive reversible phase transition and purification.
TfCa	BBa_259263IW	Coding	1491	Encodes the intermediate product MHET hydrolase, an enzyme that degrades PET hydrolysis intermediates.
Tfh	BBa_25SAE1A7	Coding	780	Encodes the PET-degrading enzyme Tfh, responsible for the initial hydrolysis of PET plastic.
SpyTag	BBa_25UXW78O	Coding	39	A peptide tag is encoded to form a covalent bond with SpyCatcher for protein anchoring.
SpyCatcher	BBa_25K73HX1	Coding	348	A protein domain is encoded to specifically recognize and covalently bind to the SpyTag peptide.
SnoopTag	BBa_259AEIGK	Coding	39	A peptide tag is encoded for interaction with SnoopCatcher to facilitate protein heterodimer assembly.
SnoopCatcher	BBa_25DIL6RB	Coding	339	A protein domain is encoded to specifically recognize and bind the SnoopTag peptide for ordered protein assembly.
Linker	BBa_25DWWUE8	Protein_Domain	33	A flexible peptide is encoded to be used as a spatial separator between functional domains in a fusion protein.
TphA1	BBa_25IB1IC9	Coding	1011	TphA1 forms together with TphA2 and TphA3 the terephthalic acid 1,2-dioxygenase system (TERDOS).
TphA2	BBa_256CWYU7	Coding	1252	TPA 1,2-dioxygenase oxydase subunit alpha.
TphA3	BBa_258YI2Y4	Coding	468	TPA 1,2-dioxygenase (TPADO) subunit beta (UniProt: Q3C1D4) encoded by the tphA3II gene, is one of the three proteins that form the TPADO complex.
TphB	BBa_25OEPQGZ	Coding	951	TphB codes 1,2-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate dehydrogenase(DCDDH), which can hydrolyze 1,2-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate to protocatechuate.
gcl	BBa_2548X1RK	Coding	1870	Catalyzes the condensation of two glyoxylate molecules to form tartronic semialdehyde. This reaction is the key carbon-carbon bond formation step in the ethylene glycol (EG) assimilation pathway.
hyi	BBa_25S9ID4S	Coding	777	Catalyzes the isomerization of hydroxypyruvate to tartronic semialdehyde, serving as a key bridging reaction that connects upstream glycolate metabolism to the downstream glycerate pathway for central metabolic integration.
glxR	BBa_25C5WV92	Coding	879	An alternative glyoxylate reductase that reduces tartronic semialdehyde to glycerate, serving a similar metabolic role to GarR in channeling carbon flux from glycolate assimilation into central metabolism.
glxK	BBa_25E9JQQL	Coding	1146	Phosphorylates glycerate to generate 2-phosphoglycerate (2-PG), enabling its direct entry into the glycolytic pathway for energy production and growth.
garR	BBa_25IF2P7B	Coding	891	Reduces tartronic semialdehyde to glycerate. It serves as a crucial step in channeling carbon from the EG pathway into central metabolism.
phaA	BBa_25CJPJQX	Coding	1197	Condenses two acetyl-CoA molecules to form acetoacetyl-CoA, the first committed step in the biosynthesis of the ester monomer for PEA.
phaB	BBa_25GN3JOD	Coding	756	Reduces acetoacetyl-CoA to (R)-3-hydroxybutyryl-CoA, generating the activated ester monomer used for PEA polymerization.
phaC	BBa_250CGRZL	Coding	1695	Catalyzes the copolymerization of (R)-3-hydroxybutyryl-CoA (ester monomer) and β-alanyl-CoA (amide monomer) to form the poly(ester amide) (PEA) polymer.
pct540	BBa_25L57841	Coding	1593	Functions as a CoA-transferase that re-activates the free acid form of the ester monomer, (R)-3-hydroxybutyrate, to its CoA-thioester form, enhancing monomer recycling and polymerization efficiency.
Act	BBa_25G4M7NG	Coding	1212	Activates β-alanine by attaching a CoA moiety to form β-alanyl-CoA, the activated amide monomer for polymerization.
panD	BBa_25DAKZS4	Coding	471	Decarboxylates L-aspartate to produce β-alanine, providing the essential precursor for the amide monomer of PEA.
fucO	BBa_2531KHUP	Coding	1149	Oxidizes ethylene glycol (EG) to glycolaldehyde, initiating the first step of EG metabolism.
aldA	BBa_25601S3B	Coding	1440	Further oxidizes glycolaldehyde to glycolate, continuing the activation of EG for downstream assimilation.
Pm93	BBa_25Z7HO0F	Promoter	87	A strong constitutive promoter that drives high-level, continuous expression of downstream genes without requiring induction, ensuring robust enzyme production in the engineered pathway.

Composition Parts

Name	ID	Type	Length(bp)	Function
T7 Promoter-lac operator-RBS-ELP-linker-SpyCatcher-6xHis-T7 Terminator	BBa_255YB1F5	Translational unit	1092	The ELP-SpyCatcher fusion protein is synthesized from this part for carrier functionalization and temperature-responsive immobilization.
T7 Promoter-lac operator-RBS-SpyTag-Linker-TfCa-Linker-SnoopTag-T7 Terminator	BBa_251W7L7C	Translational unit	1750	This part expresses a TfCa fusion protein engineered with SpyTag and SnoopTag for directed immobilization and enzyme assembly.
T7 Promoter-lac operator-RBS-SnoopCatcher-Linker-Tfh-6xHis-T7 Terminator	BBa_25RCF92Y	Translational unit	1250	A SnoopCatcher-Tfh fusion protein is produced by this part, designed to form a heterodimer with the TfCa fusion enzyme.
T7 Promoter-lac operator-RBS-TphA1-RBS-TphA2-RBS-TphA3-T7 Promoter-lac operator-RBS-TphB-T7 Term	BBa_25IQN8NN	Translational unit	3988	Carry and express the enzymes required for the conversion of terephthalic acid (TPA) into protocatechuic acid (PCA).
Pm93+RBS+gcl+RBS+garR+Pm93+RBS+glxK+Term	BBa_25MV7LOL	Device	4191	This device is the key module supporting the core finding of this study: a model-driven, minimized pathway design. It challenges the canonical four-gene paradigm by implementing a rational“gcl-garR-glxK”circuit, where the isozyme GarR replaces GlxR and the Hyi step is strategically omitted to optimize glyoxylate flux. Its equivalent performance to the four-gene baseline validates the power of model-guided pathway refactoring.
Pm93+RBS+gcl+RBS+hyi+Pm93+RBS+glxR+RBS+glxK+Term	BBa_253CDWT2	Device	4985	This device serves as a control module that reconstitutes the standard four-gene pathway reported in literature. Its purpose is to provide a performance benchmark for direct experimental comparison, enabling a clear evaluation of our minimized three-gene design against the established baseline.
Pm93+RBS+fucO+RBS+aldA+Term	BBa_25CO18KO	Device	2769	This module functions as an aldehyde scavenger and redox-balancing unit. Constitutive co-expression of AldA (aldehyde dehydrogenase) and FucO (alcohol dehydrogenase) establishes a substrate cycle that can oxidize toxic aldehydes to their corresponding acids while managing the intracellular NADH/NAD+ ratio. This is critical for maintaining redox homeostasis during the bioconversion of C2 substrates.
Trc promoter+RBS+phaB+RBS+panD+Trc promoter+RBS+phaA+rrnB-T2-Term	BBa_25Y458VZ	Device	2593	This device serves as the core monomer supply module for PEA biosynthesis, engineered for high-yield, balanced co-production of ester and amide precursors. Unit 1 constitutively co-expresses PhaB and PanD to generate the key PEA monomers. Unit 2 expresses PhaA to enhance precursor supply flux. This module provides a balanced substrate pool for downstream polymerization.
AraBAD promoter+RBS+phaC+AraBAD promoter+RBS+pct540+RBS+Act+rrnB-T1-Term	BBa_25W7TTDX	Device	5153	This device functions as an inducible polymerization module, catalyzing the copolymerization of monomers into PEA. It employs dual AraBAD promoters for independent regulation of the native PHA synthase (PhaC) and a broad-substrate hybrid synthase (Pct540-Act). Upon induction, it assembles diverse monomers into custom PEA polymers.

Exsiting parts

Name	ID	Type	Length(bp)	Function
T7 Promoter	BBa_K5328015	T7	19	Used to initiate and regulate the expression of downstream genes.
lac operator	BBa_K428400	Regulatory	25	Used for repressor-mediated control of downstream gene expression.
6xHis	BBa_K5487119	Miscellaneous (Deprecated)	18	6xHis affinity tag.
T7 Terminator	BBa_K5248006	Terminator [SO:0000141]	48	Terminator for bacterial expression.
AraBAD promoter	BBa_K4657003	promoter	285	araBAD promoter is an E. coli promoter that is tightly controlled by inducer L-arabinose and repressor Arac.
trc promoter	BBa_K4800000	promoter	30	Our Ptrc was used to provide enhanced translation initiation and high-level expression in Escherichia coli.
rrnB-T1-Term	BBa_K3033016	Terminator [SO:0000141]	87	Derived from pBAD24, and synthesized through IDT. This codes for a rrnB T1 Terminator site
rrnB-T2-Term	BBa_K3763043	Terminator [SO:0000141]	28	The rrnB T1T2 terminator, derived from E. coli ribosomal RNA genes and containing multiple inverted repeats, is an efficient factor-independent transcription termination sequence widely used in cloning vectors to prevent spurious expression.