Safety and Security
Our strategies have been carefully designed to emphasize both user safety and environmental responsibility. This approach primarily utilizes biological materials and recycled substances, such as ethidium bromide. These components are managed in a way that prevents any negative environmental impact. To uphold ethical standards and ensure the integrity of our strategy, we consulted with experts in the field, who verified that the target we developed operates in the best interest of users without causing mutations. These professionals have confirmed that the project is both safe and ethical.
The main objective of our project is to propose an alternative treatment to drug resistant Klebsiella pneumoniae. To develop this strategy, we used the microorganism Escherichia coli NEB-5-alpha and DH5-alpha, commonly found in global environments and classified as BSL-1 bacteria. Once the genes were inserted, we would have carefully followed with the expression in a BSL-2 laboratory environment, as aerobactin is considered a virulence factor and could pose a threat to the experimenter. All potentially hazardous chemicals were handled with the utmost care in a controlled environment (flow hood), and we strictly adhered to general laboratory safety protocols throughout our work.
BSL1 Setup
This is the BSL1 lab space used for experiments that come before the expression of aerobactin.
BSL1 Setup
This is the BSL1 lab space used for experiments that come before the expression of aerobactin.
BSL2 Setup
This is the BSL2 lab space that would have been used for aerobactin expression.
All experiments conducted adhered to standard procedures, including DNA electrophoresis, DNA extraction, and cloning. Among the various protocols used, we incorporated ethidium bromide—a substance widely recognized for its carcinogenic and mutagenic properties. Because of this, our team members underwent thorough training (SIMDUT and CL2 training) before handling it, ensuring that we minimized any associated risks.
Throughout the project, we upheld a strict commitment to laboratory safety. Experts closely monitored our safety protocols [1], guaranteeing that our workspace complied with both national and university regulations. Every experiment was performed under the supervision of professionals with extensive experience in the field. Furthermore, we implemented various risk management measures, such as maintaining accident reports, providing personal protective equipment, securing proper storage for laboratory materials, and establishing a comprehensive waste management system. We also emphasized ergonomic work practices, adapting postures to protect the health and well-being of our team members.
The iGEM Ulaval team is deeply committed to promoting sustainable health initiatives, inspired by recent frameworks like the Okanagan Charter [2]. This charter guides our efforts, positioning our team as leaders in sustainable health within the Francophone community.
References
- Guide de sécurité en laboratoire. (2024, 15 avril). Service de Sécurité et de Prévention. https://www.ssp.ulaval.ca/matieres-dangereuses/guide-de-securite-en-laboratoire/
- Charte de l’Okanagan | Santé durable | Université Laval. (s. d.). https://www.ulaval.ca/sante-durable/charte-de-lokanagan