Basic Parts

BioBricks	Name	Function	Result
BBa_25PGQT1D	ompA	Signal peptide, derived from the outer membrane protein A of Escherichia coli (strain K12), ompA is a short peptide chain composed of specific amino acid sequences. It is mainly located at the N-terminal of outer membrane protein A of E. coli. Its core function is to guide the protein to be correctly positioned on the extracellular membrane.	Confirmed in BL21(DE3) strains.
BBa_25KQM55E	LYS2	Signal peptide, derived from Lysis protein for colicins E2 and E3 of Escherichia coli. Its core function is to guide the protein to be correctly positioned on the extracellular membrane.	Confirmed in BL21(DE3) strains.
BBa_25FMKH14	TRAT3	Signal peptide, derived from TraT complement resistance protein of Escherichia coli. Its core function is to guide the protein to be correctly positioned on the extracellular membrane.	Confirmed in BL21(DE3) strains.
BBa_252ANKAM	FAEE	Signal peptide, derived from Chaperone protein faeE of Escherichia coli. Its core function is to guide the protein to be correctly positioned on the extracellular membrane.	Confirmed in BL21(DE3) strains.
BBa_25G71YDM	Amy	Signal peptide, derived from Alpha-amylase of Bacillus licheniformis. Its core function is to guide the protein to be correctly positioned on the extracellular membrane.	Confirmed in BL21(DE3) strains.
BBa_25KCPKBB	ydhT	Signal peptide, derived from Mannan endo-1,4-beta-mannosidase of Bacillus licheniformis. Its core function is to guide the protein to be correctly positioned on the extracellular membrane.	Confirmed in BL21(DE3) strains.
BBa_256MNFLJ	Linker(GSGSGS)	Flexible linker, Gly's small side chain provides high flexibility, Ser increases water solubility.	Confirmed in BL21(DE3) strains.
BBa_25Y1T7O4	BlJ24	Chitinase (gh18A) derived from Bacillus licheniformis j24	Not Confirmed
BBa_25K3P716	Blchi	Chitinase derived from Bacillus licheniformis ATCC 14580. Molecular Function: catalytic activity, Biological Process: carbohydrate metabolism. It is speculated based on amino acid sequence comparison that it has endonuclease activity.	Confirmed in BL21(DE3) strains.
BBa_25ZD70VG	Bschi	Chitinase derived from Bacillus subtilis. It is speculated based on amino acid sequence comparison that it has endonuclease activity.	Confirmed in BL21(DE3) strains.
BBa_25GA0H7E	Smchi	Chitinase derived from Serratia marcescens ; involved in extracellular degradation of chitin.	Not Confirmed
BBa_257MX9NY	Linker(GGSGGS)	Flexible linker, Gly's small side chain provides high flexibility, Ser increases water solubility.	Confirmed in BL21(DE3) strains.
BBa_25N93S1J	6xHis	The six consecutive histidine residues fused at the C-terminal (carboxyl-terminal) of the target protein can enable the purification of the protein.	Confirmed in BL21(DE3) strains.
BBa_25RKD52L	hSQS	Squalene synthase derived from Homo sapiens farnesyl-diphosphate farnesyltransferase 1 (FDFT1)	Confirmed in DH5α strains.
BBa_25BR1Q9C	RBS	Ribosome binding site, a key regulatory element for gene expression in prokaryotes.	Confirmed in DH5α strains.
BBa_2562VODX	AtSQE	The squalene monooxygenase derived from Arabidopsis thaliana converts the linear hydrocarbon molecule "squalene" into "2,3-oxidized squalene".	Confirmed in DH5α strains.
BBa_25LTIACW	EtAs	The beta-amyrin synthase derived from Euphorbia tirucalli catalyzes the cyclization of 2,3-oxidized squalene into β-amyrin.	Confirmed in DH5α strains.
BBa_251YYQPX	atoB	The acetoacetyl-CoA synthetase (atoB) derived from Escherichia coli catalyzes the conversion of acetyl-CoA to acetoacetyl-CoA.	Confirmed in DH5α strains.
BBa_251RESRX	tHMGR	The 3-hydroxy-3-methylglutaryl CoA reductase derived from Saccharomyces cerevisiae catalyzes the reduction of 3-hydroxy-3-methyl-valeryl CoA (HMG-CoA) to generate mevalonate (MVA), which is a crucial step in the mevalonate pathway.	Confirmed in DH5α strains.
BBa_257IFZCW	PGK1t	The terminator of the phosphoglycerate kinase 1 (PGK1) gene.	Confirmed in DH5α strains.

Composite Parts

BioBricks	Name	Function	Result
BBa_25ZAASV5	T7 promoter-lac operator-RBS-sfGFP-T7 terminator	The composite component uses the T7/LAC system as an inducible promoter to drive the expression of sgGFP, and its expression sequence is assembled according to the standard mode of "promoter + RBS → coding sequence → terminator".Functionally, the expression of fluorescent protein can be controlled artificially by adding IPTG.	Confirmed in BL21(DE3) strains.
BBa_25J819FF	T7 promoter-lac operator-RBS-ompA-linker-sfGFP-T7 terminator	The complex uses the T7/lac system as an inducible promoter, and adds IPTG to drive the expression of sfGFP. The expression sequence is assembled according to the standard pattern of "promoter + RBS → coding sequence → terminator". Functionally, the signal peptide ompA at the N-terminal can guide fluorescent proteins to the outer membrane and secrete them to the extracellular.	Confirmed in BL21(DE3) strains.
BBa_25T5PW7S	T7 promoter-lac operator-RBS-Amy-linker-sfGFP-T7 terminator	The complex uses the T7/lac system as an inducible promoter, and adds IPTG to drive the expression of sfGFP. The expression sequence is assembled according to the standard pattern of "promoter + RBS → coding sequence → terminator". Functionally, the signal peptide Amy at the N-terminal can guide fluorescent proteins to the outer membrane and secrete them to the extracellular.	Confirmed in BL21(DE3) strains.
BBa_25FLX4K5	T7 promoter-lac operator-RBS-ydhT-linker-sfGFP-T7 terminator	The complex uses the T7/lac system as an inducible promoter, and adds IPTG to drive the expression of sfGFP. The expression sequence is assembled according to the standard pattern of "promoter + RBS → coding sequence → terminator". Functionally, the signal peptide ydhT at the N-terminal can guide fluorescent proteins to the outer membrane and secrete them to the extracellular.	Confirmed in BL21(DE3) strains.
BBa_25X7ADU7	T7 promoter-lac operator-RBS-LYS2-linker-sfGFP-T7 terminator	The complex uses the T7/lac system as an inducible promoter, and adds IPTG to drive the expression of sfGFP. The expression sequence is assembled according to the standard pattern of "promoter + RBS → coding sequence → terminator". Functionally, the signal peptide LYS2 at the N-terminal can guide fluorescent proteins to the outer membrane and secrete them to the extracellular.	Confirmed in BL21(DE3) strains.
BBa_2573DEYV	T7 promoter-lac operator-RBS-FAEE-linker-sfGFP-T7 terminator	The complex uses the T7/lac system as an inducible promoter, and adds IPTG to drive the expression of sfGFP. The expression sequence is assembled according to the standard pattern of "promoter + RBS → coding sequence → terminator". Functionally, the signal peptide FAEE at the N-terminal can guide fluorescent proteins to the outer membrane and secrete them to the extracellular.	Confirmed in BL21(DE3) strains.
BBa_25XLRH1P	T7 promoter-lac operator-RBS-TRAR3-linker-sfGFP-T7 terminator	The complex uses the T7/lac system as an inducible promoter, and adds IPTG to drive the expression of sfGFP. The expression sequence is assembled according to the standard pattern of "promoter + RBS → coding sequence → terminator". Functionally, the signal peptide FAEE at the N-terminal can guide fluorescent proteins to the outer membrane and secrete them to the extracellular.	Confirmed in BL21(DE3) strains.
BBa_25BNQWPT	T7 promoter-lac operator-RBS-ompA-linker-Blchi-linker-6xHis-T7 terminator	This complex uses the T7/lac system as the induction promoter and adds IPTG to drive the expression of chitinase. The expression sequence is assembled following the standard pattern of "promoter + RBS → coding sequence → terminator". Functionally, the signal peptide ompA located at the N-terminus can guide the chitinase to reach the outer membrane and secrete it outside the cell.	Confirmed in BL21(DE3) strains.
BBa_257SYTKG	T7 promoter-lac operator-RBS-ompA-linker-Bschi-linker-6xHis-T7 terminator	This complex uses the T7/lac system as the induction promoter and adds IPTG to drive the expression of chitinase. The expression sequence is assembled following the standard pattern of "promoter + RBS → coding sequence → terminator". Functionally, the signal peptide ompA located at the N-terminus can guide the chitinase to reach the outer membrane and secrete it outside the cell.	Confirmed in BL21(DE3) strains.
BBa_25LK7NK1	T7 promoter-lac operator-RBS-ompA-linker-Smchi-linker-6xHis-T7 terminator	This complex uses the T7/lac system as the induction promoter and adds IPTG to drive the expression of chitinase. The expression sequence is assembled following the standard pattern of "promoter + RBS → coding sequence → terminator". Functionally, the signal peptide ompA located at the N-terminus can guide the chitinase to reach the outer membrane and secrete it outside the cell.	Not Confirmed
BBa_259RZK1N	T7 promoter-lac operator-RBS-ompA-linker-BlJ24-linker-6xHis-T7 terminator	This complex uses the T7/lac system as the induction promoter and adds IPTG to drive the expression of chitinase. The expression sequence is assembled following the standard pattern of "promoter + RBS → coding sequence → terminator". Functionally, the signal peptide ompA located at the N-terminus can guide the chitinase to reach the outer membrane and secrete it outside the cell.	Not Confirmed
BBa_254KCXIV	Ptac-RBS-hSQS-RBS-AtSQE-RBS-EtAs-rrnBT1	The complex uses Ptac as an inducer promoter and adds IPTG to drive the tandem expression of squalene synthase, squalene oxide cyclase and amyrin synthase. Functionally, this module can construct a synthesis pathway of β-amyrin in Escherichia coli, and gradually convert FPP in Escherichia coli into squalene, 2,3-oxide squalene and β-amyrin.	Confirmed in DH5α strains.
BBa_25HLP433	Lac promoter-RBS-atoB-HMGS-tHMGR-rrnBT1	This complex uses Plac as the constitutive promoter to continuously express the key enzymes of the MEP/MVA pathway encoded by atoB, HMGS and tHMGR. Functionally, this module increases the accumulation of FPP by increasing the copy number of endogenous genes in Escherichia coli and introducing the key enzymes of the MVA pathway in Saccharomyces cerevisiae.	Confirmed in DH5α strains.
BBa_25T64Z52	Lac promoter-RBS-ERG12-ERG8-MVD1-idi-ispA-rrnBT1	This complex uses Plac as the constitutive promoter and continuously expresses the key enzymes of the MEP/MVA pathway encoded by ERG12, ERG8, MVD1, idi and ispA. Functionally, this module increases the accumulation of FPP by increasing the copy number of endogenous genes in Escherichia coli and introducing the key enzymes of the yeast MVA pathway.	Confirmed in DH5α strains.
BBa_25ZOD7RN	Pgrac-RBS-ompA-linker-Blchi-linker-6xHis-trp terminator	This complex uses Pgrac as the inducer promoter and adds IPTG to drive the expression of chitinase. Functionally, the signal peptide located at the N-terminus can guide the chitinase to reach the extracellular membrane and secrete it outside the cell.	Confirmed in BS168delta4 strains.
BBa_250LNQQZ	Pgrac-RBS-ompA-linker-Bschi-linker-6xHis-trp terminator	This complex uses Pgrac as the inducer promoter and adds IPTG to drive the expression of chitinase. Functionally, the signal peptide located at the N-terminus can guide the chitinase to reach the extracellular membrane and secrete it outside the cell.	Confirmed in BS168delta4 strains.