1. Basic Parts

Part Number	Part Name	Description	Status
BBa_25O2B17G	GPS	This basic part is the coding sequence (CDS) of GPP synthase (GPS/GPPS), which catalyzes the condensation of IPP and DMAPP into GPP (geranyl pyrophosphate) inside the cell.	Successful PCR of Escherichia coli colony, successful expression and activation
BBa_25K8S57E	CsTPS1	This basic part encodes the coding sequence (CDS) of CsTPS1 (geraniol synthase), which specifically converts geranyl pyrophosphate (GPP) into geraniol.	Successful PCR of Escherichia coli colony, successful expression and activation
BBa_25ZQBVLG	GeDH	This basic part is the coding sequence (CDS) for GeDH (geraniol dehydrogenase). In the three-step cascade "GPS → CsTPS1 → GeDH" of this project, it is responsible for oxidizing geraniol into the target aldehyde product.	Successful PCR of Escherichia coli colony, successful expression and activation
BBa_25HC9783	GeDH-54883	GeDH-54883 is a four-point mutant (Q60R, L125P, F147Y, A300D). In addition to optimizing entrance electrostatics and channel rigidity, the F147Y mutation introduces an aromatic hydroxyl group, providing additional π/H-bond interactions to enhance selectivity and peak conversion rate.	Successful PCR of Escherichia coli colony, successful expression and activation
BBa_258IK945	GeDH-64117	GeDH-64117 is a multi-point mutant generated on the wild-type GeDH background, introducing Q60R, L125P, A298S, and A300D.	Model simulation, optimized protein
BBa_25PJ74PH	GeDH-54456	GeDH-54456 is a four-point mutant (Q60L, L125P, F147Y, A300Q).	Model simulation, optimized protein
BBa_25OX8KML	GeDH-63928	GeDH-63928 is a triple mutant (Q60R, A298T, A300D). It maintains the hydrophobic background of the channel while moderately increasing polarity and entrance electrostatics, making it suitable for robust flux enhancement and process scale-up.	Model simulation, optimized protein

2. Composite Parts

Part Number	Part Name	Description
BBa_25FBLC3S	P_T7-lacO - RBS - GPS - T7 Terminator	This composite part replicates the core expression logic of pET-28a(+): it employs the inducible strong promoter T7-lacO, paired with a strong RBS, and terminates transcription with the T7 terminator.
BBa_25ACTZLO	P_T7-lacO-RBS-CsTPS1-T7 Terminator	This composite part is driven by the T7-lacO inducible strong promoter to express CsTPS1 (Geraniol Synthase). It is assembled in the standard order of“promoter →RBS→target gene→terminator,”functionally equivalent to the core expression unit of pET-21a.
BBa_25DYJEGO	P_BAD-RBS-GeDH-Terminator	This composite part employs PBAD as a titratable inducible promoter to drive the expression of GeDH (Geraniol Dehydrogenase), assembled in the standard order “Promoter → RBS → Coding Sequence → Terminator”.
BBa_25M8PEZ2	PBAD-RBS-GeDH-54883-Terminator	This composite part uses PBAD (BBa_I0500) as the promoter to drive expression of a rationally engineered GeDH-54883 variant (Q60R / L125P / F147Y / A300D). It is assembled in the standard order “promoter → RBS → gene of interest → terminator” and mapped onto the AraC/L -arabinose regulatory framework of pBAD23.