| BBa_K5371006 | GAL4 | Coding | A gene from Saccharomyces cerevisiae S288C encoding a galactose-responsive transcription factor |
| BBa_K2637056 | GAL80 | Coding | A gene from Saccharomyces cerevisiae S288C encoding a transcription regulator which inhibits transcriptional activation by Gal4p |
| BBa_K3173005 | STE2 | Coding | A gene from Saccharomyces cerevisiae S288C encoding the Mating factor alpha receptor |
| BBa_25Y5QTBF | STE12 | Coding | A gene from Saccharomyces cerevisiae S288C encoding a component of signal pathway operating downstream of STE2 |
| BBa_25KNR1N3 | MjDOD | Coding | MjDOD was selected as the reporter gene to generate the visual output.This enzyme oxidizes L-DOPA to betalamic acid, Betalamic acid spontaneously reacts with endogenous amino acids or externally supplied amine donors, producing various colored betaxanthins |
| BBa_25W6SH7R | Pir1p | Coding | Pir1p was selected to anchor the N-terminus of the substrate tag to the cell wall |
| BBa_253NFXE9 | SNAP25 | Coding | Synaptosome-associated protein of 25 kDa (SNAP-25) serves as the substrate for botulinum neurotoxin type A (BoNT/A). BoNT/A cleaves SNAP25 at residues 197–198, thereby releasing the Mating factor alpha at the C-terminus of the substrate tag |
| BBa_25CSFL8E | Mating factor alpha | Coding | Upon proteolytic release by BoNT/A or TEV protease, the α-factor binds to the Ste2 receptor on the plasma membrane of Signal-transferring Strain, thereby initiating downstream signal transduction that culminates in activation of the reporter gene |
| BBa_25JA50EQ | URA3 marker gene | Coding | Enables orotidine-5'-phosphate decarboxylase activity. Involved in 'de novo' pyrimidine nucleobase biosynthetic process and UMP biosynthetic process. It is routinely employed for screening of the target yeast strain |
| BBa_J435273 | ScHIS3-marker | Cloning Plasmid | The plasmid carries the selection marker HIS3 |
| BBa_K4873002 | pUC19 | Cloning Plasmid | Cloning vector with an ampicillin resistance marker |
| BBa_K5471003 | pUC57 | Cloning Plasmid | Cloning vector with an ampicillin resistance marker |
| BBa_25YQ5CKG | pYES2 | Shuttle Plasmid | High-copy episomal vector for galactose-inducible expression of proteins in Saccharomyces cerevisiae |
| BBa_25XSHZU8 | pUC19-STE2-URA3 | Cloning Plasmid | A plasmid constructed to store donor sequence designed to knock out STE2 |
| BBa_2544NN57 | pUC19-GAL80-HIS3 | Cloning Plasmid | A plasmid constructed to store donor sequence designed to knock out GAL80 |
| BBa_258H678M | pUC19-GAL4-URA3 | Cloning Plasmid | A plasmid constructed to store donor sequence designed to knock out GAL4 |
| BBa_25FYVHU9 | GAL1 promoter | Promoter | An inducible promoter which is widely used in yeast protein expression system. It is tightly repressed in the presence of glucose and strongly induced in the presence of galactose |
| BBa_K3126010 | CYC1 terminator | Terminator | This part encodes the transcriptional terminator of the CYC1 gene from Saccharomyces cerevisiae. Ensures proper transcription termination and polyadenylation of mRNA transcripts |
| BBa_25SZUVYI | TEV protease cleavage site–α-factor encoding region | Coding | A gene from Saccharomyces cerevisiae S288C alpha type encoding a Alpha-factor mating pheromone.Since botulinum toxin is legally prohibited from use in our experiments, we employed TEV protease as a surrogate.Therefore we added the cleavage site of TEV protease |
| BBa_25C0C6QI | pUC19-FAR1-URA3 | Cloning Plasmid | A plasmid constructed to store donor sequence designed to knock out FAR1 |
| BBa_K1470002 | GAL4 DBD | Coding | GAL4 DNA-binding domain used to create a fusion protein called sTF, which triggers the transcription of reporter gene |
| BBa_25555HKK | STE12 PRD | Coding | STE12 pheromone-responsive domain used to create a fusion protein called sTF, triggering transcription of reporter gene |
| BBa_K5477001 | pSTE12 | Promoter | Promoter of STE12, activated by MAPK signaling cascade, responsible for inducing mating and pseudohyphal/invasive growth genes |
| BBa_256CPPNO | ste12 terminator | Terminator | Terminator of STE12, encoding a component of signal pathway operating downstream of STE2 |
| BBa_25VY4NHQ | pYES2-PGAL1-MjDOD-CYC1 | Plasmid | This plasmid can preliminarily validate the feasibility of the pigment synthesis scheme. The enzyme can convert L-DOPA in the culture medium into betaxanthin. After transforming this plasmid into yeast, addition of galactose can also induce the reaction |