As part of our project, we developed several composite parts, using existing parts in the iGEM Registry of Standard Biological Parts and parts described in various literature entries, which we adapted and had them custom-made by DNA synthesis companies. Below is an overview of our composite parts and the basic parts used to build them. TRI-LYTAC is our entry for Best Part Collection.

# Module Part Name Type Description Source / Sequence Origin
1 TRI-LYTAC AT8 scFv Basic Antibody fragment recognizing pTau (S202/T205, AT8 epitope) Literature (Mercken 1992, PDB 6O6F template)
2 HAI peptide (TfR-binding) Basic Shuttle peptide for transferrin receptor–mediated transcytosis Literature (Yu 2011)
3 IGF-2 dimer (F19L variant) Basic Ligand for CI-M6PR lysosomal routing Literature (Banik 2020; Ahn 2021)
4 (G4S)₃ linker Basic Flexible linker for spacing domains Common design element
5 Igκ secretion leader Basic Signal peptide for secretion Addgene plasmids, mammalian consensus
6 TRI-LYTAC v1 Composite Full chimera: AT8–HAI–IGF-2, secreted Benchling assembly of above
7 TRI-LYTAC controls (Drop-TfR, Drop-lys, GFP binder) Composite Variants for mechanistic testing Assembled constructs
8 TauTuner LOV2 domain Basic Blue-light responsive optogenetic domain Literature (Strickland 2012)
9 PP2A catalytic subunit Basic Major phosphatase dephosphorylating tau UniProt Q15257
10 dTAG degron Basic Small molecule–inducible degradation tag Nabet 2018
11 TetR-KRAB Basic Transcriptional repressor Addgene #85435 etc.
12 TauTuner v1 Composite LOV2–(G4S)₃–PP2A–dTAG–NLS fusion Custom assembly
13 LOV2-dead mutant Improved LOV2 inactive (dark-locked) Derived mutant
14 Logic Gates pSTAT3 promoter Basic Promoter activated by inflammatory JAK/STAT signaling Literature (SAA1 gene)
15 pARE promoter Basic Antioxidant response element (Nrf2 binding) Literature (NQO1, HO-1)
16 pCHOP promoter Basic ER stress–responsive promoter Literature (GADD153)
17 GAL4-DBD Basic GAL4 DNA-binding domain Yeast GAL4
18 GAL4-AD (VP16AD) Basic Activation domain (viral protein 16) Herpes simplex VP16
19 UAS promoter (5× GAL4 sites + min promoter) Basic Upstream activating sequence Yeast GAL4 system
20 Cre recombinase Basic Site-specific recombinase Literature / Addgene
21 STOP cassette (loxP-flanked) Basic Floxed transcriptional STOP Addgene plasmids
22 Split GAL4 system Composite pSTAT3→DBD, pARE→AD, UAS→Therapeutic Custom assembly
23 Logic-commitment (pCHOP→Cre, floxed cassette) Composite ER stress irreversible switch Custom
24 Delivery & Reporting Secretion leader variants (PelB, Tat) Basic Alternative secretion tags Addgene / UniProt
25 HiBiT tag Basic 11 aa NanoLuc reporter peptide Promega
26 NanoLuc luciferase Basic Small luminescent reporter Promega
27 mNeonGreen / GFP nanobody Basic Reporter or control binder Addgene
28 Promoter→HiBiT reporters (STAT3, ARE, CHOP) Composite For promoter strength validation Custom