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Enzyme-Based Antifouling Technology
Our iGEM project aims to combat marine biofouling by targeting cp19k, a key adhesive protein used by barnacles. We express cp19k in E. coli BL21 (DE3), then test a range of proteases to identify which one(s) degrade it most effectively. The goal is to develop a sustainable, enzyme-based solution—Barnacure—as an eco-friendly alternative to toxic antifouling methods.
Marine biofouling costs billions annually. Toxic antifouling paints containing copper oxide and TBT devastate marine ecosystems through coral bleaching, shellfish die-offs, and heavy metal accumulation.
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Barnacure targets cp19k, the barnacle cement protein. We engineered E. coli to express and purify cp19k, then tested proteases like alcalase and trypsin to develop an enzyme-based degradation system.
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A biodegradable enzymatic coating that prevents barnacle adhesion naturally. Zero toxicity, 100% sustainable, protecting marine biodiversity while saving fuel and reducing emissions.
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