Basic Parts
This year’s William_and_Mary iGEM team contributed eight basic parts to the iGEM Parts Registry, including one promoter, five coding sequences and two homologous regions. All parts were modified to remove illegal restriction enzyme cut sites, ensuring full compatibility with iGEM type IIS Assembly. These components are assembled into vectors which are used to control biofilm regulation and extracellular protein secretion in Bacillus subtilis and Mycobacterium smegmatis. For detailed information on experimental design, measurement methods, and results, please refer to our Experiment, Measurement, and Results pages.
| Name | BioBrick Identifier | Type | Description |
|---|---|---|---|
| xylA promoter | BBa_25EL4H3H | Promoter | A xylose inducible promoter for Bacillus Subtilis. |
| Bacillus subtilis TasA coding region | Twin of BBa_K1701005 | CDS | A gene in Bacillus Subtilis that produces amyloid fibers in biofilms. |
| Bacillus subtilis TapA-SipW-TasA coding region | BBa_25U0RROW | CDS | A coding region from Bacillus Subtilis that produces amyloid fibers in biofilms, with BsaI restriction cut site mutated out. |
| Bacillus subtilis BslA coding region | BBa_256V3L3M | CDS | A gene in Bacillus Subtilis that codes the hydrophobic protein BslA |
| Bacillus subtilis SinI coding region | Twin of BBa_K3498007 & BBa_K4769201 | CDS | A gene in Bacillus Subtilis that codes for an antagonist to the DNA binding protein SinR which inhibits biofilm coding gene epsA-O and tasA-sipW-tapA. |
| AmyE integration region - Left arm | Twin of BBa_K4103018 | Homologous Region | A homology arm for chromosomal integration at the amyE locus of Bacillus subtilis, used for stable insertion of genetic constructs via double-crossover recombination |
| AmyE integration region - Right arm | BBa_25Z8K8MR | Homologous Region | A homology arm for chromosomal integration at the amyE locus of Bacillus subtilis, used for stable insertion of genetic constructs via double-crossover recombination |
| MSMEG_6752 | BBa_25ONKG6K | CDS | A gene codes for a cellulase enzyme endoglucanase A that breaks down cellulose in Mycobacterium smegmatis. |
Composite Parts
We contributed six composite parts to the iGEM Registry, including four regulatory modules that produce key biofilm genes in Bacillus subtilis and one in Mycobacterium smegmatis, as well as an empty integration vector backbone that enables stable chromosomal insertion in B. subtilis. The vector serves as a tool for future Bacillus engineering projects and is fully compatible with both iGEM Type IIS (RFC 1000) and BioBrick (RFC 10) standards. Together, these parts are designed for precise control of biofilm formation and surface protein expression.
| Name | BioBrick Identifier | Type | Description |
|---|---|---|---|
| MSMEG_6752 transcriptional unit | BBa_25C1GJ0F | Regulatory | A composite part that produces MSMEG_6752, the cellulase endoglucanase A from Mycobacterium smegmatis. |
| xylose inducible SinI transcriptional unit | BBa_25UXTZY4 | Regulatory | A composite part that produces SinI, a regulator for the B.subtilis biofilm regulation pathway, inducible by xylose. |
| xylose inducible TapA-SipW-TasA transcriptional unit | BBa_2537NIQ9 | Regulatory | A composite part that produces TapA, SipW and TasA, proteins that contribute to biofilm production in Bacillus subtilis, inducible by xylose. |
| xylose inducible TasA transcriptional unit | BBa_25HGA1J2 | Regulatory | A composite part that produces TasA, a key biofilm matrix protein inB.subtilis, inducible by xylose. |
| xylose inducible BslA transcriptional unit | BBa_255Q8FC0 | Regulatory | A composite part that produces BslA, a hydrophobin inB.subtilis, inducible by xylose. |
| Bacillus subtilis integration vector pBS1C-N | BBa_254G63O6 | Empty vector | An integration vector for Bacillus subtilis that integrates into the amyE locus and compatible with iGEM type IIS (RFC 1000) and BioBrick (RFC 10) standard. |