B: Basic
C: Composite
*Plasmid construct outsourced and synthesized. Cloning, transformation, and sequencing were done by our team
References
Chandler, M., Lyalina, T., Halman, J., Rackley, L., Lee, L., Dang, D., Ke, W., … Afonin, K. A. (2018). Broccoli Fluorets: Split Aptamers as a User-Friendly Fluorescent Toolkit for Dynamic RNA Nanotechnology. Molecules, 23(12), 3178–3178. https://doi.org/10.3390/molecules23123178
Li, Y., Lucci, T., Dujovne, M. V., Jung, J. K., Capdevila, D. A., & Lucks, J. B. (2024). Engineering a cell-free biosensor signal amplification circuit with polymerase strand recycling. BioRxiv : The Preprint Server for Biology, 2024.04.25.591074. https://doi.org/10.1101/2024.04.25.591074
Beabout, K., Bernhards, C. B., Thakur, M., Turner, K. B., Cole, S. D., Walper, S. A., Chávez, J. L., & Lux, M. W. (2021). Optimization of Heavy Metal Sensors Based on Transcription Factors and Cell-Free Expression Systems. ACS Synthetic Biology, 10(11), 3040–3054. https://doi.org/10.1021/acssynbio.1c00331
Filonov, Grigory S., Kam, Christina W., Song, W., & Jaffrey, Samie R. (2015). In-Gel Imaging of RNA Processing Using Broccoli Reveals Optimal Aptamer Expression Strategies. Chemistry & Biology, 22(5), 649–660. https://doi.org/10.1016/j.chembiol.2015.04.018
Long, S. (1994). METHOD 200.8 DETERMINATION OF TRACE ELEMENTS IN WATERS AND WASTES BY INDUCTIVELY COUPLED PLASMA -MASS SPECTROMETRY Revision 5.4 EMMC Version. https://www.epa.gov/sites/default/files/2015-06/documents/epa-200.8.pdf
Team:Edinburgh/Model - 2020.igem.org. (2020). Igem.org. https://2020.igem.org/Team%3AEdinburgh/Model?utm_source=
Chen SY, Zhang Y, Li R, Wang B, Ye BC. De Novo Design of the ArsR Regulated Pars Promoter Enables a Highly Sensitive Whole-Cell Biosensor for Arsenic Contamination. Anal Chem. 2022 May 24;94(20):7210-7218. doi: 10.1021/acs.analchem.2c00055. Epub 2022 May 10. PMID: 35537205; PMCID: PMC9134189.