Biological Parts

1

BBa_2523FOB1

miR-34a-5p

has-miR-34a-5p mature miRNA

Basic part

registry.igem.org/parts/bba-2523fob1

2

BBa_25ZRBPGM

miR-93-3p

has-miR-93-3p mature miRNA

Basic part

registry.igem.org/parts/bba-25zrbpgm

3

BBa_25HQZU2J

sLIRA-miR34A-5p

The sequence of the single-loop toehold nucleic acid detection system

Basic part

registry.igem.org/parts/bba-25hqzu2j

4

BBa_25TPW58J

lentiCRISPRv2_ZsG gRNA_1

This plasmid contains two expression cassettes, hSpCas9 and the chimeric guide RNA.

Basic part

registry.igem.org/parts/bba-25tpw58j

5

BBa_25XS3W32

TEAD4-gRNA sequence

gRNA sequence is designed to target the conserved exonic sequence common to these TEAD4 transcripts.

Basic part

registry.igem.org/parts/bba-25xs3w32

6

BBa_25T222K9

pCOLAD-dLIRA-miR-34a-5p/93a-p-EGFP

A detection plasmid system carrying a double-loop sequence to detect extracts from patients' serum or tissue cells

Composite 

Part

registry.igem.org/parts/bba-25t222k9

7

BBa_25AAS5PI

pCDFD-miR-93-3p/miR-34a-5p

This plasmid to induce the expression of the two miRNAs in BL21 Escherichia coli (E. coli) upon the addition of IPTG.

Composite 

Part

registry.igem.org/parts/bba-25aas5pi

8

BBa_25B9QSJX

shRNA-TEAD4

This is designed to transfect into the target cells to facilitate the knockout of the intended gene

Composite 

Part

registry.igem.org/parts/bba-25b9qsjx

9

aaa520d0-cd31-4988-af1f-04321b108765

Toehold Switch

We employed a two-input AND gate, which is a single toehold switch activated by two trigger

RNAs-miR-34a-5P and miR-93-3p for breast cancaer detection.

Part Collection

registry.igem.org/collections/aaa520d0-cd31-4988-af1f-04321b108765