Parts
New Composite Parts
Part No. Name Type Length Description
BBa_25QOUAP0 Syn-M-INPUT Test 1 Composite 25506 This part serves as the first-generation signal input module for constructing a liver-specific SynNotch system. However, it tends to exhibit relatively strong autoactivation in the absence of stimulation by the liver-specific membrane protein SLC17A2.
BBa_25MB6HBO Syn-M-INPUT Test 2 Composite 1746bp This part serves as the second-generation signal input module for constructing a liver-specific SynNotch system. Compared to the first generation (BBa_25QOUAP0), it exhibits significantly reduced autoactivation and is used in subsequent development.
BBa_25A4ZJOW Syn-M-OUTPUT EGFP Composite 993bp This part serves as the signal output module for constructing a liver-specific SynNotch system and expresses green fluorescent protein (GFP) upon activation. It is typically used in combination with the corresponding signal input module (BBa_25QOUAP0 or BBa_25MB6HBO) to form a complete SynNotch system, allowing verification of whether the input modules are specifically activated in a liver-dependent manner.
BBa_25FIPKVH Syn-M-OUTPUT P65-SIRPα shRNA Composite 2068bp This part serves as the signal output module for constructing a liver-specific SynNotch system, and upon activation, it simultaneously expresses P65 and SIRPα shRNA. Together with the corresponding signal input module (BBa_25MB6HBO), it constitutes the final design—Synergy—by the 2025 AFMU-China team. Synergy is capable of liver-specific activation rather than hepatocellular carcinoma (HCC)-specific activation, thereby overcoming the challenge posed by the heterogeneity of liver cancer antigens. Upon activation, Synergy simultaneously upregulates P65 and downregulates SIRPα expression, reprogramming macrophages into an anti-tumor M1 phenotype while blocking the "don't eat me" signal, thus addressing the challenge of the immunosuppressive tumor microenvironment.
New Basic Parts
Part No. Name Type Length Description
BBa_25NB22G5 Anti-SLC17A2 scFv-1 Coding 726bp Anti-SLC17A2 scFv-1 is a single-chain antibody targeting SLC17A2, a specific membrane protein on the surface of hepatocytes. It exhibits lower affinity and specificity for SLC17A2 than BBa_25JOL8XW.
BBa_25TIRQID Anti-SLC17A2 scFv-2 Coding 741bp Anti-SLC17A2 scFv-2 is a single-chain antibody targeting SLC17A2, a specific membrane protein on the surface of hepatocytes. It exhibits lower affinity and specificity for SLC17A2 than BBa_25JOL8XW.
BBa_25JOL8XW Anti-SLC17A2 scFv-3 Coding 726bp Anti-SLC17A2 scFv-3 is a single-chain antibody targeting SLC17A2, a specific membrane protein on the surface of hepatocytes. It exhibits higher affinity and specificity for SLC17A2 than BBa_25NB22G5 and BBa_25TIRQID.
BBa_25UUNUCS mNotch1 extracellular domain Coding 891bp The extracellular domain of mouse Notch1, containing the Furin-like cleavage site, is commonly used to construct the signal input module of the SynNotch system.
BBa_25H5A7YH mNotch1 transmembrane domain Coding 87bp The transmembrane domain of mouse Notch1, which anchors the connected structure to the cell membrane, is commonly used to construct the signal input module of the SynNotch system.
BBa_25ZV4ZAC CD8α hinge Coding 81bp The extracellular domain of mouse CD8α, which undergoes self-cleavage upon activation, is commonly used to construct the signal input module of the SynNotch system.
BBa_259GQ32O p65 Coding 1649bp p65 (RELA) is the principal transcriptional activator subunit of the NF-κB complex, orchestrating expression of genes central to immunity, inflammation and cell survival.
BBa_25FC3CBR miR-155-SIRPα-shRNA Regulatory 129bp A Pol-II–driven, miR-155-flanked shRNA that knocks down SIRPα to unleash macrophage anti-tumor phagocytosis.
General Basic Parts
Part No. Name Type Length Description
BBa_25P9MHYT TEF1 promoter Promoter 399bp The TEF1 promoter is a commonly used promoter in eukaryotic expression systems, capable of driving high-level expression of target genes.
BBa_K1470002 Galactose-induced gene 4 DNA binding domain (Gal4DBD) Coding 441bp This sequence actually expresses a chimeric protein composed of the GAL4 DNA-binding domain and the transcriptional activation domain from the herpes simplex virus protein VP16, which can specifically activate the transcription of genes located downstream of the upstream activation sequence (UAS). It can form a complete GAL4/VP16-UAS system with BBa_25JV1GYA, which has been widely used in various gene regulation studies.
BBa_25JV1GYA GAL4_UAS Regulatory 155bp The GAL4-UAS system activates downstream gene expression through GAL4 binding to UAS and recruiting the VP16 transactivation domain. This system has been widely used in various gene regulation studies. It can form a complete GAL4/VP16-UAS system with BBa_K1470002, which has been widely used in various gene regulation studies.
BBa_255XWLKI pCMV-T7 T7 116bp This sequence enables the eukaryotic application of the T7 promoter by using the eukaryotic CMV promoter to initiate the expression of both T7 RNA polymerase and the T7 promoter.
BBa_25MDFEL9 eGFP Coding 716bp Enhanced green fluorescent protein coding sequence.