Find all our experimental protocols in PDF format here.
Find our protocols contribution for iGEM in the hydrogel category
Detailed method for calculating necessary dilutions in the laboratory. This protocol includes formulas and practical examples for preparing solutions at specific concentrations.
Protocols to prepare specialized bacterial culture media. Includes DL-glutamic acid supplementation for auxotrophic strains, tyrosine plates for melanin detection, and M9 minimal media formulations with microwave sterilization methods.
High fidelity PCR amplification protocol using Q5 enzyme. Optimal conditions for specific and efficient amplification of DNA fragments.
Rapid screening method for bacterial colonies by PCR using Econotaq enzyme.
Protocol for transforming supercompetent bacterial cells with plasmid DNA. Method for obtaining high transformation efficiency.
Complete protocol for nucleic acid separation by agarose gel electrophoresis.
Method for preparing supercompetent bacterial cells for transformation. Detailed protocol to obtain high transformation efficiency.
Protocol for preparing electrocompetent bacterial cells for transformation by electroporation. Method for high transformation efficiency.
Protocol for purification of PCR amplification products and enzymatic digestions. Purification methods by column and precipitation.
Small-scale plasmid DNA extraction protocol. Method for isolating high quality plasmid DNA from bacterial cultures.
Golden Gate assembly protocol for vector construction. Standardized assembly method using type IIS restriction enzymes.
SLIC cloning protocol for assembling DNA fragments without ligation. In vitro homologous recombination method for vector construction.
DNA digestion protocol with restriction enzymes. Optimal conditions for complete and specific digestion of plasmid or genomic DNA.
Protocol for measuring optical density at 600 nm to assess bacterial growth. Standard method for monitoring bacterial culture growth.
Specific protocol for construction and analysis of murI mutants. Methods for inactivation of the chromosomal murI gene and resulting phenotypic analysis.
Our contribution to iGEM is this complete protocol for hydrogel preparation using alginate and protocol for bacterial encapsulation.
Our contribution to iGEM is detailed protocol for testing the escape of bacteria in hydrogel
Our contribution to iGEM is complete protocol for testing the survival of bacteria in hydrogel.