Thrombosis formation and dissolution have a profound impact on human cardiovascular health and aquaculture economics. Given hirudin's significant thrombolytic efficacy, our project focused on the scientific exploration of hirudin to achieve recombinant expression of Hirudin protein for downstream applications. Through comprehensive database mining, we identified 12 hirudin homologs. Expression vectors for Hirudin production in E. coli were designed and constructed. Cloning, bacterial transformation, and IPTG-induced expression successfully yielded a functional Hirudin protein. Affinity chromatography purification is the next step we wish to take in continuing this project. This study demonstrates notable innovation potential in expanding the application scope and refining the production methodologies of hirudin.