PROJECT HARDWARE

This device is engineered for the quantitative estimation of nitric oxide (NO) concentration in blood samples. Its operation is based on the specific fluorescence response of a proprietary fluorescent protein upon binding with NO molecules.

Core Components:

• Optical Detection Module: A high-sensitivity camera serves as the primary sensor. It is equipped with a dedicated optical filter to precisely capture the fluorescence emission from the sample while completely blocking the scattered excitation light.
• Excitation Source: An ultraviolet (UV) lamp module is designed to emit light at two distinct, precise wavelengths. This dual-wavelength excitation is crucial for ratiometric measurement, which enhances accuracy by compensating for potential sample variability.
• Sample Presentation Unit: A specialized glass slide ensures optimal presentation of the prepared sample. The slide is crafted from materials with low fluorescence background and high UV transmission to maximize signal-to-noise ratio.

The device follows a systematic four-step process to accurately quantify nitric oxide concentration in blood samples:

1. Sample Preparation: The blood sample is pre-mixed with the specific fluorescent protein marker, which selectively binds to NO molecules present in the sample.
2. Loading: The prepared mixture is placed onto the designated glass slide and carefully inserted into the device's sample compartment, ensuring proper positioning for optimal optical detection.
3. Excitation & Data Acquisition: The UV lamp sequentially illuminates the sample at the two predefined wavelengths. For each wavelength, the high-sensitivity camera captures the corresponding fluorescence intensity, creating a pair of measurements for ratiometric analysis.
4. Signal Processing & Analysis: The device's integrated processor calculates the ratio of the two fluorescence intensities. This ratio is then correlated to the NO concentration through a pre-calibrated algorithm, yielding a final estimated value that is displayed to the user.

The design and workflow of this device fully embody the three core principles of our solution: The fully automated four-step detection process with integrated intelligent algorithms delivers true Intelligent Detection; its compact modular design and universal consumables ensure Universal Accessibility, enabling deployment across diverse settings from tertiary hospitals to primary care clinics; the simplified operational steps and intuitive result output perfectly demonstrate User-Centric Design, allowing even non-specialists to perform accurate testing with ease.