© 2025 - Content on this site is licensed under a Creative Commons Attribution 4.0 International license.
The repository used to create this website is available at gitlab.igem.org/2025/biotech-xjuh.
On this page, You can view all the genetic parts we designed and used in our project, including two DNA fragments and four plasmid sequences. In this section, we will provide an overview of each plasmid, explaining its basic characteristics, construction details, and specific role within our experimental design.
| Part number | Description |
|---|---|
| BBa_25ACT8L2 | alkane 1-monooxygenase gene, derived from Rhodococcus erythropolis strain XP, is a gene which can express protein AlkB to digest n-alkanes. Because of this property, it is expected to transfer into our engineering bacterium and expresses enzyme to decompose low molecular weight polyethylene. |
| BBa_258ZQQ4D | The gene cluster of alkane 1-monooxygenase gene. (alkB) The sequence is accessed from GenBank, part of Rhodococcus erythropolis strain XP genome. multiple cds can improve the efficiency of decomposing n-alkanes. it is also expected to transfer into our engineering bacterium and expresses enzyme to decompose low molecular weight polyethylene. |
| BBa_25QHIQJB | Plasmid pUWL201 is a shuttle plasmid which can be used in gene transfer from Ecoli. to Streptomyces. It has ampicillin resistance gene and Thiostrepton resistance. |
| BBa_250DT46K | This is a composite part. The gene alkB is cloned to pUWL201 by using EcoRI and HindIII enzyme cutting. The plasmid is design to be transferred into Streptomyces and E.coli. to express gene alkB. |
| BBa_25MXQ5FF | This is a composite part which is similar to BBa_250DT46K. The gene alkB cluster is cloned to pUWL201 by using EcoRI and HindIII enzyme cutting. The plasmid is design to be transferred into Streptomyces and E.coli. to express the whole gene cluster. |
| BBa_25EK93WX | This plasmid was synthesized by GenScript and is used for heterologous expression experiments in E. coli, specifically to express the alkB gene and degrade heptadecane (C17 alkane). |
© 2025 - Content on this site is licensed under a Creative Commons Attribution 4.0 International license.
The repository used to create this website is available at gitlab.igem.org/2025/biotech-xjuh.
