Acknowledgement

We acknowledge SnapGene for providing software support through their sponsorship, which facilitated plasmid design, annotation, and documentation throughout our project.

We also thank Dr. Robert Britton and Dr. Duolong Zhu from Baylor College of Medicine for providing the plasmid map of pBSI2 (Zhu et al., 2025), which guided us to improve the performance of calprotectin biosensor.

References

↑ Zhu, D., Galley, J., Pizzini, J., Musteata, E., Douglas, M. V., Chazin, W. J., Skaar, E. P., Tabor, J. J. & Britton, R. A. (2025). Microbial Biosensor for Sensing and Treatment of Intestinal Inflammation. Advanced Science, e2504364. https://doi.org/10.1002/advs.202504364