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Basic Parts
Name Number Type Description
Information Input Module
CmeR BBa_256YK45O Coding This part encodes a repressor that responds to taurocholic acid (TCA)
pCmeR(-10) BBa_257TP3PQ Promoter This part is a CmeR-regulated promoter that places cmeO downstream to -10 motif
pCmeR(-35) BBa_259Y8QCZ Promoter This part is a CmeR-regulated promoter that places cmeO to overlap with -35 motif
pCmeR(spacer) BBa_25DKNIQO Promoter This part is a CmeR-regulated promoter that places cmeO in the spacer between -35 and -10 motifs
TcpH BBa_25L370I2 Coding This part encodes a co-factor protein that protects TcpP from degradation
CadC-TcpP BBa_2597H89T Coding This part encodes a TCA sensor compatible with E. coli
pCadBA BBa_25FYZ24I Promotor This part is a inducible promoter activated by CadC-TcpP
ykgMO (fixed) BBa_25J2ZYTL Promoter This part is a promoter responsive to calprotectin
Zur BBa_25PFX2AS Coding This part codes for the zinc uptake regulator (Zur)
Functional Output Module
KrLCB12 BBa_25JQNEX5 Coding This part encodes the keratinase KrLCB12
KrLCB12 (without signaling peptide) BBa_2597O3NS Coding This part encodes the signal peptide removed version of the keratinase KrLCB12
KrMKU3 BBa_25J6XNWA Coding This part encodes the keratinase KrMKU3
KrMKU3 (without signaling peptide) BBa_25WHQJ10 Coding This part encodes the signal peptide removed version of the keratinase KrMKU3
KrOcL9 BBa_25VB4UDR Coding This part encodes the keratinase krOcL9
KrOcL9 (without signaling peptide) BBa_25C6M1U8 Coding This part encodes the signal peptide removed version of the keratinase krOcL9
KrUS575 BBa_25EY32UM Coding This part encodes the keratinase KrUS575
Data Storage Module
Cas2 BBa_25P738AS Coding This part encodes the integrase responsible for prespacer integration into the CRISPR array
Cas1(E269G) BBa_25JT0SQG Coding This part encodes the integrase responsible for prespacer integration into the CRISPR array
Cas1(V76L) BBa_253BEGMH Coding This part encodes the integrase responsible for prespace integration into the CRISPR array
Cas1(V76L E269G) BBa_25Q74ZK1 Coding This part encodes the integrase responsible for prespace integration into the CRISPR array
msrRNA BBa_2536MF02 RNA This part is the intermediate Eco1 RT template in the Retro-cascorder system
msd (barcode A) BBa_25SODRDA RNA This part records gene expression events in cells under aTc induction
msd (barcode B) BBa_2597PZJ2 RNA This part records gene expression events in cells under choline induction
Retron Ec86 RT BBa_256Y16ZC Coding This part encodes Retron Eco1 Reverse Transcriptase

Composite Parts
Title BBa_ Type Description
Information Input Module
J23119-CmeR(mut)_pCmeR(-10)-B0031-GFPmut2 BBa_25F4UCQM device This device is the first iteration of the CmeR-based taurocholic acid (TCA) sensor
J23119(mut)-CmeR_pCmeR(-10)-B0031-GFPmut2 BBa_25A12OMH device This device is the second iteration of the CmeR-based taurocholic acid (TCA) sensor
J23119(mut)-CmeR_pCmeR(-35)-RBS(Bgl)-sfGFP BBa_25E1I21E device This device is the third iteration of the CmeR-based taurocholic acid (TCA) sensor
J23119(mut)-CmeR(2*TAA)_pCmeR(-35)-RBS(Bgl)-sfGFP BBa_256FO3O6 device This device is the third iteration CmeR-based taurocholic acid (TCA) sensor with nonsense mutation introduced in CmeR
J23119(mut)-CmeR_pCmeR(spacer)-RBS(Bgl)-sfGFP BBa_25RC9Z6P device This device is the fourth and final iteration of our CmeR-based taurocholic acid (TCA) sensor
J23119(mut)-CmeR(2*TAA)_pCmeR(spacer)-RBS(Bgl)-sfGFP BBa_25UQXNE7 device This device is the fourth iteration CmeR-based taurocholic acid (TCA) sensor with nonsense mutation introduced in CmeR
TcpP-sfGFP BBa_25XJ1XUG device This device is a TCA sensor based on CacC-TcpP and pCadBA
ykgMO-sfGFP BBa_2506ZYC1 device This device is a calprotectin sensor (ykgMO) and a GFP reporter
Zur-ykgMO-sfGFP BBa_25IKJDQ6 device This device is a calprotectin sensor with ykgMO IGS (fixed) and zinc uptake regulator (Zur)
Information Processing Module
pAND BBa_25NC4UDH device This device is a transcriptional AND-gate from linked transcriptional regulators
T7-RNAP strong BBa_25IM9T2N device This device is a Split T7 AND-gate with strong RBSs before RNAP fragments
T7-RNAP weak BBa_25TBOJB2 device This device is a Split T7 AND-gate with weak RBSs before RNAP fragments
T7-reporter strong BBa_25UKROJT device This device is a Split T7 AND-gate with strong RBS before reporter sfGFP
T7-reporter weak BBa_2538U45C device This device is a Split T7 AND-gate with weak RBS before reporter sfGFP
One-plasmid Split T7 AND Gate BBa_25YVR8VN device This device is a RBS-optimized version of the Split T7 protein-level AND-gate
Functional Output Module
lacI-KrLCB12 BBa_25LTONDB device This device encodes the keratinase KrLCB12 under IPTG induction
lacI-KrLCB12 (without signaling peptide) BBa_25EWD96Y device This device encodes the keratinase KrLCB12 without signaling peptide under IPTG induction
lacI-KrMKU3 BBa_256G166P device This device encodes the keratinase KrMKU3 under IPTG induction
lacI-KrMKU3 (without signaling peptide) BBa_25074FYM device This device encodes the keratinase KrMKU3 without signaling peptide under IPTG induction
lacI-KrOcL9 BBa_25QXN0XE device This device encodes the keratinase KrOcL9 under IPTG induction
lacI-KrOcL9 (without signaling peptide) BBa_25UMKEOP device This device encodes the keratinase KrOcL9 without signaling peptide under IPTG induction
lacI-KrUS575 BBa_25EK3S9T device This device encodes the keratinase KrUS575 under IPTG induction
Data Storage Module
pSBK.079 BBa_25X8951T plasmid This plasmid is a recording plasmid in Retro-Cascorder system
pSBK.134 BBa_253V08M1 plasmid This plasmid is a recording plasmid in Retro-Cascorder system
pEcolRT_T7-Cas1(WT)-Cas2 BBa_25593RLH plasmid This plasmid is a plasmid carrying the Cas1 wild type
pEcolRT_T7-Cas1(E269G)-Cas2 BBa_25VCQRET plasmid This plasmid is a plasmid carrying the Cas1 variant Cas1 (E269G)
pEcolRT_T7-Cas1(V76L)-Cas2 BBa_25WYL7QM plasmid This plasmid is a plasmid carrying the Cas1 variant Cas1 (V76L)
pEcolRT_T7-Cas1(V76L E269G)-Cas2 BBa_25OKVR2V plasmid This plasmid is a plasmid carrying the Cas1 variant Cas1 (V76L E269G)
pEcCas BBa_25T8TRM7 plasmid This plasmid is a low-copy pSC101-based vector carrying the Cas9 nuclease and the λ-Red recombination system
pEcgRNA BBa_25PAAV39 plasmid This plasmid is a high-copy ColE1 plasmid that expressing a single guide RNA (sgRNA) under a strong constitutive promoter

Contribution Parts
Title BBa_ Type Description
INPNC BBa_K811003 Protein_Domain This part encodes a truncated version of the iced nucleation protein (INP)
TES4 BBa_K3838613 Coding This part is a Tes4-related part

Part Collection

For parts in our part collection, the design of the collection as a whole, and system-level characterization, refer to our part collection.