VLP-Coupled High-efficiency ATP
Re-Generating Enzyme system
We
Revolutionised the Industrial Pathway
for
Cell-Free Biosynthesis
Cell-free biosynthesis enables the efficient and flexible production of diverse biomolecules, demonstrating immense potential in the field of industrial biosynthesis.
Cell-free biosynthesis
However, the high cost of ATP supply and poor energy stability represent one of the core challenges limiting its large-scale application.
Relevant studies have shown that phosphate accumulation, pH fluctuations, and protease interference all inhibit the activity of ATP regenerating enzymes, potentially leading to a 50%–80% decline in yields during prolonged reactions.
To address this,
we introduce the V-CHARGEs platform.
We used the polyphosphate kinase SIPPК, which exhibits high activity at the optimal temperature and can use low-cost polyphosphate (PolyP) as a substrate.
We encapsulated SIPPК with the porous P22-VLP capsid, which can effectively protect and maintain the activity of SIPPК while enabling the cyclic regeneration of ATP.
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V-CHARGEs represents an innovation in enzyme engineering
within traditional cell-free biosynthesis.
Application scenarios
V-CHARGEs!
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