Bba |
Part Name |
Type |
Length(bp) |
Designed by |
Prize |
BBa_25ST8FLD |
PLA2 |
basic-coding |
843 |
BSDFZ-CHINA |
Silver |
BBa_25USFVFA |
TAL |
basic-coding |
654 |
BSDFZ-CHINA |
- |
BBa_256H06VX |
4CL |
basic-coding |
1,710 |
BSDFZ-CHINA |
- |
BBa_25NCLJJ7 |
CHS |
basic-coding |
1,167 |
BSDFZ-CHINA |
- |
BBa_25I7VX04 |
CHI |
basic-coding |
666 |
BSDFZ-CHINA |
- |
BBa_25886WFT |
TAL-4CL-CHS-CHI |
composite-coding |
- |
BSDFZ-CHINA |
- |
BBa_259M31WV |
F7-OMT |
basic-coding |
1,056 |
BSDFZ-CHINA |
- |
BBa_25MTY54W |
CysE |
basic-coding |
819 |
BSDFZ-CHINA |
- |
BBa_25Z8A0VX |
ydaO operon |
basic-Regulatory |
1577 |
BSDFZ-CHINA |
- |
BBa_250J0XUW |
OMT-ydaO-cysE |
composite-coding |
- |
BSDFZ-CHINA |
Gold |
BBa_25MIOUT0 |
OMT-ydaO |
composite-coding |
- |
BSDFZ-CHINA |
- |
BBa_K808000 |
pBAD |
basic-promoter |
- |
04_MIT |
Bronze |
BBa_K4987003 |
pCspA |
basic-promoter |
- |
23_Thinker-Guangdong |
- |
BBa_254YB8C8 |
T4 Holin |
basic-coding |
657 |
BSDFZ-CHINA |
- |
BBa_25L8X41M |
T4 Lysozyme |
basic-coding |
492 |
BSDFZ-CHINA |
- |
BBa_25VR3TWB |
pBAD-T4 holin-T4 Lysozyme |
composite-coding |
- |
BSDFZ-CHINA |
- |
BBa_25R1N9DT |
pCspA-T4 holin-T4 Lysozyme |
composite-coding |
- |
BSDFZ-CHINA |
- |
BSDFZ-CHINA 2025: Neurobloom – Probiotic-based Early Alzheimer’s Intervention
This year, the BSDFZ-CHINA team focused on developing a next-generation probiotic capsule, Neurobloom, designed to provide early intervention for Alzheimer’s disease. Our engineered E. coli Nissle 1917 strain integrates sakuranetin biosynthesis, LPC production, and a dual-layered safety system, forming a living pharmaceutical factory that targets the gut-brain axis. Altogether, we contributed 11 new basic parts and 5 new composite parts, which cover flavonoid biosynthesis, lipid metabolism, metabolic regulation, and biosafety.
Core Contributions
Basic Parts
- BBa_25ST8FLD (PLA2, 843 bp) – encodes phospholipase A2, catalyzing the conversion of phosphatidylcholine into LPC, a neuroprotective lipid involved in membrane repair and choline metabolism.
- BBa_25USFVFA (TAL, 654 bp) – tyrosine ammonia-lyase, initiating the naringenin pathway by converting tyrosine into p-coumaric acid.
- BBa_256H06VX (4CL, 1710 bp) – 4-coumarate:CoA ligase, activating p-coumaric acid into p-coumaroyl-CoA.
- BBa_25NCLJJ7 (CHS, 1167 bp) – chalcone synthase, generating the flavonoid backbone (chalcone).
- BBa_25I7VX04 (CHI, 666 bp) – chalcone isomerase, cyclizing chalcone into naringenin.
- BBa_259M31WV (F7-OMT, 1056 bp) – O-methyltransferase, methylating naringenin to sakuranetin, a bioactive flavonoid with anti-inflammatory potential.
- BBa_25MTY54W (CysE, 819 bp) – serine acetyltransferase, boosting SAM availability for efficient methylation.
- BBa_25Z8A0VX (ydaO operon, 1577 bp) – ATP-sensing riboswitch plus vhb and ptxD, dynamically regulating energy metabolism to stabilize sakuranetin yield.
- BBa_K808000 (pBAD) – arabinose-inducible promoter, enabling controlled induction of suicide genes.
- BBa_K4987003 (pCspA) – cold-shock promoter, activated at 16 ℃ for temperature-dependent containment.
- BBa_254YB8C8 / BBa_25L8X41M (T4 Holin & T4 Lysozyme, 657 & 492 bp) – phage lysis proteins forming the basis of our safety system.
Composite Parts
- BBa_25886WFT (TAL-4CL-CHS-CHI) – a 4-gene flavonoid module producing naringenin from tyrosine.
- BBa_25MIOUT0 (OMT-ydaO) – couples F7-OMT with ydaO operon to optimize sakuranetin methylation.
- BBa_250J0XUW (OMT-ydaO-CysE, Gold Prize) – integrates OMT, ydaO, and CysE for maximized sakuranetin yield via enhanced methyl donor supply.
- BBa_25VR3TWB (pBAD-T4 Holin-T4 Lysozyme) – inducible suicide system triggered by L-arabinose.
- BBa_25R1N9DT (pCspA-T4 Holin-T4 Lysozyme) – cold-inducible suicide system ensuring environmental safety.
Benefits for Future iGEM Teams
Our work demonstrates how probiotic chassis can be programmed for therapeutic metabolite production while maintaining biosafety. Key contributions include:
- A modular naringenin–sakuranetin pathway, overcoming low-yield issues in traditional extraction and chemical synthesis.
- A novel PLA2-LPC module, providing a synthetic biology route to neuroprotective lipid metabolites.
- An energy-sensing riboswitch system (ydaO operon), offering future teams a tool for balancing high-demand biosynthetic pathways.
- A dual-layered suicide switch (pBAD + pCspA), a flexible design for both manual clearance and environmental auto-containment.
Together, these innovations extend beyond Alzheimer’s disease: they provide reusable parts and design strategies for projects involving flavonoids, bioactive lipids, metabolic optimization, and microbial safety control.
