| BBa_25GIFA8M |
SlpA signal peptide (Lactobacillus) |
Encodes the SlpA signal peptide
(MKKNRLIVSAAAAALLAVAPVAAS) used to route heterologous proteins to the Sec secretion
pathway in Gram-positive bacteria such as
Lactobacillus. |
| BBa_255A3G83 |
Igκ signal peptide (mammalian) |
Encodes the Igκ signal peptide (METDTLLLWVLLLWVPGSTG) for N-terminal fusion to proteins, directing them into the endoplasmic reticulum and secretion into the extracellular medium in mammalian systems. |
| BBa_25M9LXNV |
anti-IL-23 scFv (ustekinumab-derived) |
Encodes an anti-IL-23 scFv based on the variable regions of ustekinumab, an FDA-approved monoclonal antibody targeting the IL-12/23 p40 subunit. Arranged as VH–(G4S)₃–VL for minimal monomeric expression and antigen binding. |
| BBa_25K895MQ |
anti-IL-23 scFv (risankizumab-derived) |
Encodes an anti-IL-23 scFv based on the variable domains of risankizumab, a clinically validated antibody targeting the p19 subunit of IL-23. Arranged as VH–(G4S)₃–VL to preserve paratope geometry in a monomeric format. |
| BBa_25D0JS44 |
anti-IL-23 scFv 1 |
Encodes a de novo designed scFv against IL-23 in VH–(G4S)₃–VL format. When fused to a secretion signal, the scFv is secreted into the supernatant and detected by anti-His Western blot, showing dose-dependent IL-23 binding in ELISA. |
| BBa_25A5L3IO |
anti-IL-23 scFv 2 |
Encodes a de novo IL-23-binding scFv in VH–(G4S)₃–VL format. Designed for secretion and detection by anti-His Western; validated by ELISA showing dose-dependent IL-23 binding. |
| BBa_25C0CV3I |
anti-IL-23 scFv 3 |
Encodes a third de novo IL-23-binding scFv (VH–(G4S)₃–VL). When fused to a secretion signal, the scFv is secreted into the supernatant and shows specific, dose-dependent IL-23 binding in ELISA. |
