Our Contribution:
Our team has been certain to document our methods and protocols in as much detail as possible so that this experiment may be replicated or used as a reference for future iGEM teams. Our successful assembly of a construct and cloning through the protocols we followed and adjusted can be utilized by other teams who wish to achieve a certain or similar result, but are unsure of what protocol to follow to obtain it. Our protocols are documented in full detail and have been recorded for future reference, for other teams or our own.
ETSU's iGEM team has used a means of achieving our template (locational promoter, promoter to achieve a certain function, plasmid) that is efficient and can be applied in an exceptionally broad variety of experiments. Our template itself (pCNHP, PSYpro, RUBY) is exceptionally versatile and opens the door for many possibilities, such as swapping elements of the template or replacing variable regions, and primarily, adding locational seed promoters.
