Contribution

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Wet Lab Protocols

Wet Lab Contributions
The iGEM Guelph wet lab team has provided optimized protocols for Golden Gate assembly using the MoClo-YTK throughout the design, building, and testing of the various parts of our regulatory genetic circuit. This protocol is useful for iGEM teams and labs that look to complete Golden Gate assembly using the New England Biolabs BsaI-HFv2 restriction enzyme and HI-T4 DNA ligase. Additionally, we have also laid the groundwork and begun the assembly of a library of modular parts that may be used for future testing of modified regulatory circuits that can be designed to detect a variety of ligands and output unique responses. For more information, please visit Experiments and Parts for more information.

Hardware Lab Notebook

Hardware Protocols

Dry Lab Contributions
The iGEM Guelph dry lab team has developed detailed protocols and documentation for the design, fabrication, and testing of the filter medium containment system. These protocols outline standardized methods for preparing, freeze-drying, and rehydrating agarose-based scaffolds, incorporating nutrient gradients and zeolite additives, and assessing permeability, yeast adhesion, and biofilm formation. Additionally, the team provides step-by-step guides for constructing and testing a 3D-printed containment chamber optimized for water filtration experiments. These resources offer valuable reference for future iGEM teams interested in developing biofiltration projects that use genetically modified organisms for bioremediation. For more information, please visit Hardware and Engineering for additional details.

Utilize our resources to facilitate your own outreach events!

Grade 5 & 6 Life Science Curriculum Supplement
Lesson plans and resources that align with Ontario’s Life Science Curriculum for Elementary students in grades 5 and 6.

Animal Health Slideshow

Animal health lesson outline

Human Health Slideshow

Human health lesson outline

Microbes and Me Workshop
Run a workshop for children aged 9-11 to introduce microbiology with fun activities.

Personal Microbes Slideshow

Microbes & Me Monday

Lab Safety Protocols

Biosecurity Escape Room Guide
An easy-to-follow and affordable guide to running a biosecurity themed escape room. To learn more about how it is set up, visit the Education page for more information.
University of Guelph iGEM Escape Room Guide

School of Witchcraft and Wizardry
Science is magical! Run the following activities with a wide variety of age groups to introduce the basics of synthetic biology, learning about DNA and engaging with microbiology.
School of Witchcraft and Wizardry Outline

DIY Curriculum Treasure Boxes
Check out an example of how you can make a curriculum Tresure Box to send to classrooms! Below is a sample of an Ontario curriculum life science activity box for the first grade.

Host your own LinkedIn Night
Who said a biology degree was straight to unemployment? Run a workshop with lower year undergraduates in the biological sciences to up their LinkedIn gain and get networking.

Lab to LinkedIn Slideshow

Lab to LinkedIn Outline