Number	Name	Description
BBa_K5096058	AmpR	Ampicillin Resistance Gene
BBa_25GO3H8G	KanR	Resistance gene is used as a selectable marker, allowing...have successfully incorporated foreign DNA into the genome.
BBa_25VJ6I1R	pcT	Propanoyl-CoA transferase (PCT) is an enzyme that catalyzes the transfer of CoA groups between molecules, playing a crucial role in metabolic pathways involving short-chain fatty acids.
BBa_25DJAP0G	phaA	This Gene derived from Limnospira platensis NIES-46 encodes β-ketothiolase, which catalyzes the condensation of two acetyl-CoA molecules to form acetoacetyl-CoA. This is the initial rate-limiting step in the synthesis of β-hydroxybutyrate (BHB). The gene has been codon-optimized for expression in E. coli.
BBa_K089002	phaB	NADPH-dependent acetoacetyl-CoA reductase catalyzes the stereospecific reduction of acetoacetyl-CoA to (R)-3-hydroxybutyryl-CoA, a key intermediate in the polyhydroxybutyrate (PHB) and BHB biosynthetic pathway. The sequence used has been optimized for high expression in E. coli.
BBa_25VNN84E	rplO	This Gene encodes the 50S ribosomal protein L15, a component of the large ribosomal subunit involved in protein synthesis.
BBa_K5048028	ccdB	ccdB (Control of Cell Death protein B) is a toxin component of the ccdAB toxin-antitoxin system in bacteria. It inhibits DNA gyrase activity, causing lethal DNA damage in sensitive cells. In synthetic biology, ccdB is often used as a selection marker in cloning strategies and plasmid maintenance systems.
BBa_K5048031	LuxI	LuxI is part of the quorum-sensing system in Vibrio fischeri. It synthesizes acyl-homoserine lactone (AHL), which diffuses across the cell membrane and acts as a signaling molecule.
BBa_K5048032	LuxR	LuxR is a transcriptional regulator that binds to the AHL synthesized by LuxI. The LuxR-AHL complex then binds to the Lux promoter (Plux), activating the transcription of downstream genes as part of the quorum-sensing regulatory mechanism.
BBa_K5048037	Plux	Lux promoter is a promoter activated by the LuxR-AHL complex in the quorum-sensing system of Vibrio fischeri. It serves as a key regulatory element for AHL-controlled genetic circuits.
BBa_25RKKVBP	PhlF promoter	The PhlF promoter is a TetR-family repressor-regulated promoter originally characterized in Bacillus subtilis. It is regulated by the PhlF protein, which binds to the DNA and represses transcription. When PhlF binding is inhibited, the promoter is activated. This promoter is often used in synthetic biology for building tightly regulated expression systems, logic gates, and biosensors.
BBa_25Z0BO02	phlF	PhlF is a TetR-family transcriptional repressor protein that regulates gene expression by binding to specific DNA sequences. It is commonly used in synthetic biology as a regulatory element to control the transcription of target genes, often in conjunction with promoters that are specifically responsive to PhlF.
BBa_25NO1FNH	CI repressor + ssrA-tag	The fusion construct combines the λ phage CI repressor with an ssrA degradation tag. The CI repressor functions as a transcriptional regulator by binding to operator sequences in a dimeric form, thereby repressing transcription. The addition of the ssrA tag facilitates rapid protein degradation via the cellular proteolysis machinery, helping to quickly reduce the intracellular concentration of CI when expression is halted. This design enhances the dynamic control of gene circuits by preventing residual CI from lingering in the system.
BBa_K4307000	OR1-OR2	The Right Operator Region (OR1-OR2) from bacteriophage lambda is used as a regulatory module for controlling gene expression in synthetic circuits. It contains operator sequences that can be bound by the CI repressor to modulate the activity of downstream promoters.
BBa_K387003	PfdhF	The fdhF promoter is an anaerobically induced promoter that regulates the expression of genes involved in formate metabolism under low-oxygen conditions. It is used in synthetic biology to drive gene expression in anaerobic or microaerobic environments.
BBa_K2272000	promoter VHb	It is the strongest promoter induced under microaerobic conditions and can efficiently regulate the expression of VHb (bacterial hemoglobin) and other genes. This promoter is used in synthetic biology to enhance metabolic activity and gene expression under low-oxygen conditions.
BBa_25TZUMQM	Forward primer of phaA	It is the forward primer of phaA used for amplifying the phaA gene fragment in PCR.
BBa_25ELXH9O	Reverse primer of phaA	It is the reverse primer of phaA used for amplifying the phaA gene fragment in PCR.
BBa_25G3HSHC	Forward primer of phaB	It is the forward primer of phaB used for amplifying the phaB gene fragment in PCR.
BBa_25VJQ9PQ	Reverse primer of phaB	It is the reverse primer of phaB used for amplifying the phaB gene fragment in PCR.
BBa_25EK44GZ	Forward primer of pcT	It is the forward primer of pcT used for amplifying the pcT gene fragment in PCR.
BBa_2585BS9H	Reverse primer of pcT	It is the reverse primer of pcT used for amplifying the pcT gene fragment in PCR.
BBa_25M9A2RX	Forward primer of rplO	It is the forward primer of rplO used for amplifying the rplO gene fragment in PCR.
BBa_25IG0DWC	Reverse primer of rplO	It is the reverse primer of rplO used for amplifying the rplO gene fragment in PCR.
BBa_25RXJ4ZP	Forward primer of SUMO	It is the forward primer of SUMO used for amplifying the SUMO gene fragment in PCR.
BBa_254U2D9W	Reverse primer of SUMO	It is the reverse primer of SUMO used for amplifying the SUMO gene fragment in PCR.