Lab Experiments

Our drug’s biosynthetic design is a key factor in its innovation and accessibility. By utilizing fusion peptides synthesized through advanced recombinant technologies, the production process is significantly streamlined compared to conventional biologics, for instance, monoclonal antibodies. This biosynthetic method enables precise control over peptide sequences and functions, ensuring high specificity and effectiveness in targeting glaucoma's pathological mechanisms.

Furthermore, the biosynthetic strategy grants rapid optimization and modification of the peptide sequences to enhance stability, solubility, and bioavailability. This adaptability accelerates development timelines and facilitates improvement based on clinical feedback, ultimately refining the therapeutic profile. Overall, the biosynthetic foundation of our drug not only advances glaucoma treatment but also sets a new standard for efficient, accessible peptide-based therapeutics.

Our team had developed FT, BC recombinant plasmids and conducted a series of lab experiments. We conducted gel electrophoresis to verify our gene. Our plasmids have been successfully transformed to 3 different E.coli strains, DH5-α, BL21 and SHuffle cells. Our targetted peptides have been expressed as expected. After that, we have done SDS-PAGE to ensure correct protein size. We also underwent BCA to find out our peptide concentration. We have successfully done ELISA to figure out the standard curve, the intense yellow colourings have proven the efficient binding between our peptides and targeted receptors, the binding concentration of our peptides with receptors has also been found.

Through the development and dissemination of novel components that promote synthetic biology research, the team has made noteworthy contributions to the iGEM community. Through careful design, thorough testing, and clear documentation, the team has added new components to the iGEM Parts Registry, encouraging accessibility and cooperation. Through careful design, testing, and documentation, the team has introduced new components to the iGEM Parts Registry, fostering accessibility and collaboration. These initiatives aim to foster creativity and expand opportunities for future synthetic biology research, empowering researchers worldwide.

Our Educational Initiatives in iGEM

In our attempt to promote Project Opthera and the importance of synthetic biology to the public, we’ve had several publications, and resources to aid us with this goal in mind.

Promotional Publications

To target the public, we designed eye-catching leaflets, a storybook, and an eye care book to introduce synthetic biology concepts, as well as highlighting the importance of eye care.These materials not only provide foundational knowledge about synthetic biology, but also help stimulate their interest in science and inspire them to choose or devote themselves to the field of synthetic biology. The storybook contains the explanation of images, which can simplify difficult knowledge, make it easier for the public to understand, arouse readers' curiosity, attract them to continue reading, and deepen their understanding of synthetic biology. The eye care book contains a large amount of professional data, which can make the public aware of the importance of eye protection, encouraging them to take actions to prevent eye diseases early, and maintain a healthy life.

Interactive Learning Tools

Our team created an original video game and two board games: ECM Hunter, Genetic Journey, and Opthera The Game. These games serve as a dynamic educational tool, allowing students to explore the world of synthetic biology engagingly. This interactive platform encourages hands-on learning, making complex concepts more approachable and fascinating. We use games to foster curiosity and deepen understanding among younger audiences, aiming to engage them in the field of synthetic biology in the future.

Multimedia Resources

We uploaded a series of informative videos, PowerPoint presentations, and posts to complement our educational materials on our media accounts. These resources cover topics related to synthetic biology and laboratory equipment. By providing accessible materials, we aim to foster a broader understanding of synthetic biology and its implications for our world. This knowledge empowers individuals to engage critically with scientific advancements. By uploading these materials to the Internet, the public can easily access them, allowing this knowledge to inadvertently enter their minds and help them learn more about the field of synthetic biology.

For more details on our educational initiatives, please refer to the Education page.