Human Practices

Human Practices


  We believe that educating public about plastic pollution and environmental justice would benefit our environment in the long run. To raise awareness on environmental pollution, we organized a beach cleanup activity not only to pick up plastic waste and general wastes in the beach, but also teach students about types of microplastic, how to collect and count the number of different types of microplastics. The collected data were sent to the Big Microplastic Survey, a global citizen science research project which gathers essential data about distribution of microplastics and mesoplastics on coastlines. Besides, we organized sharing sessions to primary school students in the youth center in order to reach a wider and more diverse audience. Students from different local primary schools shared different perspectives which enriched the discussion. We strive to engage school students to have hands-on learning outside the school classroom to help protect the environment and contribute data to science research. Such learning helps students to build confidence in thinking of new solutions and motivating to act.



Beach Cleanup

In April 2025, we organized a beach cleanup in Wu Kai Sha beach, with over 70 students joining the event to collect waste items and perform microplastics collection in line with the Big Microplastic Survey guidelines. There were 7 research teams and 5 waste collection teams. Each team was led by iGEM team students. To make the experience more rewarding, we conducted Pre-field trip and identified waste items on the beach in order to make a guideline to teach students the type of plastic items that can be recycled. We also asked students to record the number and types of collected items. The information will be useful for comparison with future beach cleanup activities.










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Youth Center Sharing Session

In May, our team visited The Hong Kong Federation of Youth Groups Felix Wong Youth S.P.O.T. and gave a sharing session to primary school students. We started off by welcoming the kids who ranged from all primary year levels and introducing our team. Then, we did a pre-survey to understand how kids of various ages perceived topics such as biosensors, artificial intelligence, and environmental justice. The ultimate goal of the sharing session was to educate younger students about environmental issues and what our team is doing this year to make an effort into resolving these issues.


It is challenging to deliver abstract concepts like biosensors and artificial intelligence in our project to primary school students. Thus, we used two innovative elements, build a BioBrick game and interactive video to convey the core idea.


The research team explaining what biosensors were, and the basic structure of a BioBrick circuit (Promoter 🡪 RBS 🡪 CDS 🡪 Terminator) that was simplified into different shapes, we let the kids have a go at re-arranging the different parts to see if they remembered the sequence of a basic BioBrick circuit, which gave them a deeper understanding of how the BioBrick circuit functioned as well as providing an interactive activity which grabbed the attention of kids at such a young age. Students were actively participated in the game and all groups were successfully locate the BioBrick circuit sequences. Afterwards, our human practice team played a short video, which illustrated how microplastics contaminated things around us such as food, and elaborated on how serious plastic pollution is, and the way it is affecting marine life and endangering species. Then, we introduced them the ways we could reduce plastic consumption in our daily lives, such as following the 3Rs principal, as well as what schemes the government has imposed to cut down plastic production as well as implementing proper waste treatment.


Another interactive part of this sharing session was the choice-driven video which let the audience make choices on things such as what packaging to choose and how to dispose of plastic waste, with each choice, they create a unique story and ending to the video, effectively demonstrating environmental justice as well as how to cut down on plastic usage.

We aim to transform students’ understanding about plastic pollution from a simple issue of waste management to a complex issue of social equity and corporate responsibility and help them to develop empathy and global citizenship, therefore, we integrated the concept of environmental justice in the video content.


Environmental justice is a social movement that addresses injustice on poor or marginalized communities where they’re harmed by hazardous waste, resource extraction, and other land uses from which they do not benefit. In other words, it’s a movement that fights to ensure that everyone - regardless of race, geography, or income - has fair access to a healthy environment. Justice is often absent from circular economy discussions. Regarding marine plastics, the distribution of impacts of marine plastics is unequal, because communities have different relationships with the ocean. During 2018, the amount of plastic waste China accepted reduced by 99%, Most of that waste has since been redirected to other south-east Asian countries, who do not have the capacity to handle such massive amounts of plastic waste. Therefore, we hope the advocation of environmental justices would raise awareness among students of the injustice behind the unequal burden of climate impact that marginalized communities experience and take responsibility for the environmental impact of our own actions.


The link for the interactive video is as follows:

https://www.canva.com/design/DAGnVEN8yjY/-Q74G7Ruz0-kDZsPO_-ypQ/view?utm_content=DAGnVEN8yjY&utm_campaign=designshare&utm_medium=link2&utm_source=uniquelinks&utlId=h7c3f3acb59

The script for the interactive video:

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Lastly, our technology team briefly explained the structure of our robotic car and its working principle, explaining how we ultimately chose YOLO-v8 as the AI model instead of others. We then conducted a Q&A session after the sharing session to understand how much the students absorbed and if they learnt anything from our sharing session. Students were actively participated in answering the questions and expressed their opinion which shows that students did learn more about plastic waste and environmental justice, as well as the basic sequence of a BioBrick circuit.


In this activity, we learned that some of the contents such as the design of biosensors and AI models comparison were too difficult for them. Together with the suggestion from Dr. David Jones, Founder of Just One Ocean, we learnt to adjust the level of difficulty of our presentations to our young audience and use analogy related to our daily life when presenting abstract concepts at school of different year levels. The experience was extremely helpful in preparing future sharing sessions.












Integrated Human Practices


We reached out to different stakeholders including NGO founders, University professors, Environmental Protection Department of local Government and community recycling network serving entire Hong Kong to seek their advice on our project. We gained valuable insights and suggestion on how to reshape our project ideas to make better contribution to the society, communicating the public about science and technology-based approaches to plastic pollution problem, educate them the environmental justice implications of global dependence on plastics and promote environmental sustainability through clean recycling.



Interview with Dr. David Jones from Just One Ocean

Dr. David Jones is the CEO and Founder of Just One Ocean, a charity committed to preserving ocean ecosystems and biodiversity for future generations. The mission is to protect the ocean for future generations through science, education and communication. Dr. Jones engaged in a lot of Microplastics Research & Beach Clean Up Studies to combat plastic pollution. His global citizen science initiative “The Big Microplastic Survey” is very successful to gather essential data for research study. Besides, he often speaks to the public through various talks about the problem of ocean pollution. Thus, our team seek advice for science communication, awareness of environmental justice and his thoughts about our project.



Key insights and takeaways

In the interview, Dr. Jones mentioned that research is important to increase people knowledge about the issue. Public engagement is similarly important so that everyone take responsibility. He provided us valuable insights about how to resolve different feedback from people of different age groups or educational backgrounds and how can we integrate these data into raising awareness in our project with diverse perspectives and experiences. He suggested to take flexibility in presentation approach. Adjusting presentation at different levels to different age groups and making diverse competitions to include diverse range of audiences. Besides, Dr Jones reminded us that we should educate the audience who didn’t know about or interested in the problem. Community outreach would help to connect a close link to people such as in media and to get more public awareness of our project. We also learned from Dr. Jones that key barriers need to address to achieve progress in plastic pollution problems were corporate accountability, inadequate waste management infrastructure and consumer behaviours. To reverse this crisis, not only companies need to be held accountable, but public educations are also crucial. Technological innovation is a tangible solution, but it still requires a strong government plan and decisions.


Regarding to environmental justice, Dr Jones indicated that less than 60% of the waste is recycled in the United Kingdom, and he thinks that there is poor waste management in Less Developed Countries (LDCs), and discharging waste there is unethical. He reminded us that waste management has a wider ethical dimension. When we asked about how companies can be controlled to address the problem of plastic pollution, he said that green parties in Europe have discovered this problem, and now plastic pollution has been controlled in different places.


Regarding to our research project, we asked Dr. Jones if there were any kits or tools allow easier detection of microplastics to his best knowledge and he reminded us that the public needs to know what is in our biosensors. People want to use our biosensors because they are interested in it, instead of testing for microplastics before drinking a bottle of water, also, there has to be a purpose, the thing we are collecting etc., he also thinks that our biosensors will be beneficial to scientific research, but there has to be a reason of testing for microplastics. He shared that there is an enzyme that digests plastics, and people thought it was enough to solve plastic pollution, but these kinds of innovations are not the entire solution to plastic pollution. A holistic approach with different aspects needs to include to address the problem.


When we mentioned the Artificial Intelligence aspect of our robotic car, we asked if we should focus exclusively on A.I.


In Conclusion, Dr. Jones mentioned that as technology advances, the use of Artificial Intelligence is inevitable, but we should utilize it as an assisting tool, while maintaining traditional research methods. To conclude, Dr David Jones believed that community outreach is important to get more people to know about our team and microplastics. In addition, we need to define the purpose of our biosensor to let the public know what we are doing. Our team need to collect more data from beach cleanups while testing the functionality of the AI robotic car.



Implementation

We integrated Dr. Jones suggestion to make our presentation level flexible to suit for different level of students. We host sharing session in primary schools and secondary schools with different presentation levels after Dr. Jones interview. For primary school students, we made the sharing session more funny, interactive and easy to understand. We modified our ppt used in Youth center by using larger and colourful pictures and breakdown the concepts into separate slides. For example, we used colourful shape to represent basic components of BioBrick circuit and taught students the sequence of basic BioBrick circuit (Promoter 🡪 Ribosome Binding Site 🡪 Coding Sequence 🡪 Terminator). Then we invited two groups of four students each group to come on the stage. Each of them was given a cardboard with one of the four components of BioBrick circuit. They had to arrange a correct sequence of the circuit. Students were actively participated in the game and were able to make a correct sequence.





Besides, students were fully engaged in a choice-driven video in which they make choices on decision such as what packaging to choose and how to dispose of plastic waste in which they create a unique story and ending to the video.



We also used robot performance to show the comparison results of three AI models that our team used in building the suitable model installed in the robotic car. Robot with the best score represent the best AI model performance which is YOLOv8 in our project.


Finally, we hosted a Kahoot game to collect feedback for students’ understanding of our project.

Review from Kahoot game of 250 Primary School Students (P5 & P6) showing that 77% of students like our Kahoot games (Q12). Besides, among the 11 Kahoot questions, there are 6 questions with high accuracy rate of over 70%. The remaining 5 questions have a relatively low accuracy rates in which the lowest accuracy rate question is about the 2 main parts of biosensor and the accuracy rate is 27% only. Others including the signal molecule secreted by bacteria, difference between primary and secondary microplastics and design of our AI vehicles. These contents were revised to make them become more understandable in our future educational activities.


Dr. Jones mentioned that the environment justice issue concerning plastic disposal would be poor waste management in Less Developed Countries (LDCs) and discharging waste there is unethical. We integrated this advice into presentation contents and our educational booklet by showing Malaysia, one of the biggest importers of plastics as an example to let the public know the harmful effect of the injustice behind and people need to take responsibility for the environmental impact of our own actions.

Regarding to our project, Dr. Jones suggested us to define the purpose of our biosensor and collect more data for the functionality of AI robotic car. Our project idea was developing a colour strip to detect the concentration of microplastics in water. In order to know the feasibility of the use of colour strip by the public, we sent interview invitation to Environmental Protection Department (EPD) of Hong Kong Government. We also ask for their suggestion about the development of AI robotic car. We obtained valuable insights from them and reshape our project aims in the interview.



Interview with Prof. Jose Jimenez from Imperial College London

Prof. Jose Jimenez is an Associate Professor in Synthetic Biology, Department of Life Sciences, Faculty of Natural Sciences in Imperial College London. One of his research focuses involve the use of engineered microorganisms for the upcycling of plastic waste into molecules of added value. With his expertise in synthetic biology and the use of engineered microorganism to degrade microplastics in wastewater, we aimed to seek advice from him on how to improve the sensitivity of our biosensor as there was a leaky expression of β-galactosidase in the uninduced biosensor after 30 minutes of detection. Besides, we asked for his thought about how to educate and convince the public to accept the use engineered microorganisms to address microplastic pollution.



Key insights and takeaways

During our interview with Professor Jimenez, he provided us with useful and valuable advice about the design of our biosensor. He suggested us to improve the sensitivity of biosensor by testing the strength of promoters in order to improve the binding of transcription factor LasR to AHL molecules. He also mentioned about testing the specificity of our biosensor, he talked about false positives, and that it would be good to improve the specificity, ensuring the kit only detects microplastic but no other substances to eliminate the problem of wrong detection. He also suggested us to test our biosensor with both distilled water and seawater to compare the results in order to compare the engineered biosensors.


Professor Jimenez talked about people have concerns on genetically engineered products and they fear that the products will affect their health. They reluctant genetic engineering only when consumption but they are fine for pollution. He suggested that when designing our test kit, we should educate the public that genetically engineered products have low risks, and they are just identical to natural products in order to build trust towards the products within the public.


Regarding our presentations and sharing sessions. He provided education advice by giving examples of his own lectures. In his lectures, he often designs a small quiz afterwards to make sure students remember the content. He also talks about student-led courses which is having the students do research and educate each other. Moreover, he suggested that we should have different messages when communicating with different groups of students, to secondary students, we should focus more on our research project, but we should educate primary school students with science in general.



Implementation

To test the sensitivity of the biosensor, we adopted Prof. Jimenez’s suggestion to use different inducible promoters, pLasRL and pLasR3 in the biosensor design to compare their sensitivity. Inspired by his idea of increasing affinity of transcription factor LasR to AHL molecules to increase the biosensor sensitivity, we took the reverse approach to reduce the leaky expression of β-galactosidase in the uninduced biosensor by removing the transcription factor LasR in the sensing module so that no LasR-AHL complex will be formed and no complex will bind on the reporting module and thus no β-galactosidase expression. Regarding to the specificity of engineered biosensor, we implemented his idea into our project by including a total of three AHL molecules for testing the specificity. As discussed in the result, we have successfully reduced the leaky expression of β-galactosidase and showed that sensitivity of biosensor with pLasR3 promoter is higher than that with pLasRL promoter. We also test the engineered biosensor with both distilled water and seawater to compare the performance.


Regarding our education activities, Professor Jimenez suggested us to use simpler vocabularies when explaining terms like 'biosensors' and 'quorum sensing'. This suggestion led us to rewrite our scripts with simpler words, it is a huge success as students are more enthusiastic in participating after the change, their understanding was also better. He also talked about how his lectures are more discussion-based rather than having students memorizing all the content, after the inspiration, we designed more games and interactive activities in our presentations which turned out having great outcomes.





Interview with Prof. Ville Santala from Tempere University

Prof. Ville Santala is a Professor of biotechnology at Tempere University. He is a specialist in synthetic biology and industrial biotechnology and his research focus on using microbes to consume plastic waste. We aimed to ask his view about the application of our biosensor and the release of engineered bacteria into the environment to solve plastic pollution problem.



Key insights and takeaways

In the interview, Professor Ville Santala mentioned it was good to have biosensors to detect microplastics within a limited time so that treatment plants would start with locations having many microplastics. Development of microplastic detection method was an emerging trend. He suggested the method should be easily detected and measured, high availability and could be removed from the environment. Besides, he discussed in the importance in communication and collaboration other than the development of biotechnology. Without proper collaboration, nothing could come into fruition. He also believes that the public was well-educated regarding recycling methods and the plastic pollution crisis, the key to take action is on the policies, while research provides new remedies. When asking the limitation of using bacteria to digest plastic wastes, Prof. Ville Santala mentioned that production required up-scaling biological processes and cultivate bacteria in a large scale took years to upscale to industry level. He also provided us suggestions of implementing a smartphone camera on our robotic car, which could provide more function.


In conclusion, he believes that his personal impact came through from education and his views aligns with the Human Practice Team’s aims to encourage awareness of plastic pollution via education. Scientific innovation is crucial for combating the issue, constructing a better and sustainable world.



Implementation

Prof. Villi Santala mentioned that Finland has a well-established collection system to recycling or upcycling plastic wastes which inspire us to explore the plastics recycling system and the recycling rate in Hong Kong and to consider how to encourage public practising recycling and how to include relevant information into our educational activities. Thus, we invited Waste Management Division, Environmental Protection Department (EPD) of Hong Kong Government and Green@Community, a network of community recycling centers run by EPD for an interview and visit.




Interview with Environmental Protection Department (EPD) from Hong Kong Government

We had the opportunity to speak with the Hong Kong Environmental Protection Department (EPD) to better understand their current work in environmental protection, particularly in plastic pollution and their efforts in promoting sustainability. The interview with EPD was the most effective way to ensure our project has real-world impact. We aimed to thoroughly understanding the EPD’s need, the operational constraints and workflows so that we can engineer a biosensor that is not only innovative but also indispensable. We interviewed Water Quality Management Division from the EPD.



Key insights and takeaways

In the interview with Water Quality Management Division, we learned that Hong Kong Government put high priority on microplastic issues. They promote the public awareness and tackle microplastic pollution in a holistic approach.


Regarding to the promotion of public awareness in different sectors, they mentioned that microplastics was a new environmental issue occurring nearly 10 years ago. Knowledge about their effect on human health was building through research and survey. They promoted public awareness of microplastics via different channels to reach people with different backgrounds and levels of knowledge such as Facebook, featuring their mascot Big Waster, interview and episodes which help to promote EPD works in an interesting way and encourage more interactive promotion with citizens. They mentioned that microplastics were highly difficult to remove which highlighted the importance of the human practice team's role of spreading the awareness of plastic pollution. The EPD shared an experience on how they promote the idea of minimizing the usage of microplastic in industrial firms by establishing a charter called "Bye Bye Microplastic," targeting industry insiders and aiming to help everyone understand what microplastics are. At the end of this program, there was a Prize-giving Ceremony. They told us that the prize ceremony was very important because it acted as an incentive for firms, effectively motivating them to stop using microplastics in addition to legislation. Besides, the EPD has held workshops and booths to promote environmental protection and attract the public to learn about microbead pollution. They have also held beach cleanup activities, where people directly visit the beach and witness the seriousness of plastic pollution that is happening. By having this experience, the public will know what they can to help reduce the pollution. Concerning what can students or schools do to reduce microplastic pollution, EPD mentioned that spreading message to family, friends, peers and different stakeholders was very important. When everyone takes a little action, we can help the environment. The approach suggested by EPD gave us ideas about what the human practice team can do to engage people of different age groups to participate in learning our project works to tackle plastic pollution.


We asked the EPD about the current method to detect and quantify microplastics to learn about their operational constraints. They mentioned that secondary microplastics produced by the fragmentation of larger plastics are the major contributor to plastic pollution. It was not efficient to collect microplastics as they were too small to be detected and collected. The most effective way was water management and microplastic detection in order to prevent microplastics reaching the water bodies. However, current detection method was labour consuming and expensive. The method involved sample collection of 1000 L seawater and 500 L lake water by Manta trawling, sample pretreatment and identification by counting visually under a stereomicroscope. However, the detected microplastics was less than 1 per 1 L. They mentioned that the procedural charge including net system, equipments and shipping cost was expensive and therefore regular monitoring was impossible. Besides, the quantification methods varied from different countries making inconsistency in the global comparison. Therefore, our engineered biosensor has potentials to allow microplastic detection in a rapid, low cost and regular basis. It also allows consistent comparison of microplastic pollution across different countries. They mentioned that a good biosensor should have high specificity and sensitivity. We also need to consider the operational environment, end-user and cost of the biosensor.


For our AI model, the EPD mentioned that achieving a balance between high sensitivity and specificity is essential. This requires a large, diverse dataset, encompassing various types, colors, and shapes of microplastics, to effectively train the AI model. Besides, they suggested us to include items other than microplastics in the training dataset to ensure the model would be able to avoid false positives from other environmental contaminants. This ensures the AI can accurately identify and count only the microplastics.



Implementation

The EPD provided key insights into transitioning a product from theoretical planning to practical implementation. We learned that our biosensor provides a solution to limitations of current laboratory procedure in EPD to collect and detect microplastics. To ensure our project has wider real-world impact, we shaped our project ideas from engineering a whole-cell biosensor for making paper strip to detect microplastics in the water by the public to making a testing kit for laboratory use. With the demand of a rapid, low cost and consistent method of microplastic detection in water samples, our biosensors is feasible to deploy in EPD’s Water Management Division for routine and high-throughput microplastics monitoring and assessment in marine waters, rivers, beaches, stormwater drains and effluent from wastewater treatment plants; Drainage Service Department laboratory to manage load of microplastics entering and leaving wastewater treatment plants; Water Supplies Department laboratory to monitor reservoir water and treated drinking water for microplastic contamination to ensure public health; University research for research discovery and commercial environmental testing laboratories for providing analytical testing services to clients such as NGOs on a commercial basis.


We also include non-plastic items such as branches and leaf in microplastics image sets for AI model training to ensure the trained model can distinguish false positive from environmental contaminants.


Besides, our team adopted various approaches such as sharing session, beach cleanup, game, interactive video etc to promote the awareness of the public to environmental issues.








Interview with Waste Management Division and a visit in Green@SaiKung under the Environmental Protection Department (EPD) from Hong Kong Government

Inspired from interview with Prof. Villi Santala whose country, Finland has a well -established collection system to recycling or upcycling plastic wastes, we aimed to study the recycling system in Hong Kong and consider how our team contribute in this aspect.


We sent interview questions to Waste Management Division and received their reply. We learned that to strengthen the support for waste reduction and recycling at the community level, EPD has been expanding the community recycling network, GREEN@COMMUNITY comprising Recycling Stations, Recycling Stores and Recycling Spots, and trying out smart recycling technologies with a view to gaining more support from the public to go green and instill a green living culture in the community. GREEN@COMMUNITY collects nine common types of domestic recyclables, including plastics, waste papers, metals, glass containers, regulated electrical equipment, small electrical appliances, compact fluorescent lamps/fluorescent tubes, rechargeable batteries and beverage cartons. To avoid residents accidentally put non-recyclable items into the recycling bin which will lower the quality of the recycled products, the GREEN@COMMNUITY operators will educate the public on the Recycling Stations/Recycling Stores/Recycling Spots. Recyclables collected through the GREEN@COMMNUITY will be delivered to downstream recyclers approved by EPD for subsequent processing and turning into resources. The EPD has introduced the “GREEN$ Electronic Participation Incentive Scheme” (GREEN$ ePIS) to further encourage the public to carry out clean recycling through gift redemption incentives. Besides, GREEN@COMMUNITY Recycling Stations and Recycling Stores will upload their quarterly report of operation and collected recyclables statistics on their Facebook page. They will review the operation efficiency, spatial distribution and specific needs of individual district, etc., in a timely manner. Subject to the availability of resources, they will adjust or enhance the recycling services at district level to facilitate the public in practicing waste separation and clean recycling. To further strengthen the publicity and education of waste reduction and recycling at school level, the EPD launched the “We-recycle@School” Activity on 1 March 2024, inviting participation of all primary and secondary schools across the territory. As of June 2025, about 550 primary and secondary schools have participated in the “We-recycle@School” Activity, involving about 350 000 students.


In a visit to the EPD’s Green@Sai Kung, we see how everyday plastic items can be melted into pellets, repurposed and transformed into new products such as new plastic bottles, showcasing an effective system that not only reduces waste but also conserves valuable resources. Our team also got to learn about the various recycling schemes and recycling tips, with a quick tour of the storage room where recyclable materials are gathered to undergo further processes. Moreover, recycled plastics can be used in 3D printers to make chairs, which made us know how our society really takes a step forward to a sustainable future. Overall, the experience was really informative and engaging and highlighted the importance of recycling in our communities.



Implementation

From the interview reply and the visit to Green@SaiKung, we acknowledged EPD of HK Government to place microplastic pollution and waste recycling as top priorities and take a holistic approach in engaging school communities and public to participate in recycling and reduce wastes. We realize that there are challenges in the implementation such as how to encourage the public to practise recycling and how to educate them to achieve clean recycling. As mentioned in the interview reply, cross contamination with non-recyclable items into the recycling bin will lower the quality of the recycled products. Thus, our team collaborated with Environmental Organization initiated from our school, Zero Plastic League to organize a Green Event by hosting a green booth in our school events to educate students, teachers and parents how to achieve clean recycling and promote environmental sustainability through recycling. We also put types of recyclable items in our educational booklets to educate the public and encourage them to reduce plastic usage and recycle right. In the future, we will look for opportunities to collaborate with EPD or NGO to organize more green events to promote clean recycling and sustainability.



Human Practices | HKGTC - iGEM 2025