Overview

In HemorrEaser, we have developed an integrated parts toolkit consisting of four novel basic biological parts and three sophisticated composite devices, all specifically designed for hemorrhoid therapy and genetic circuit regulation. Examples include an anti-angiogenesis module incorporating the VHH-VHL bioPROTAC construct, and an anti-inflammatory module containing an engineered melittin expression cassette. Through systematic assembly and rigorous characterization—including sequencing verification, colony PCR, and protein expression analysis—we have confirmed the proper functionality of each individual part.

You can read them below.

Basic Parts Table

Part Number	Name	Part Description
BBa_2546SCTX	Anti-VEGF Nanobody	Anti-VEGF nanobody fused with P17 peptide and cleavable masking peptide.
BBa_25WEXRPG	OmpA-CAR	Mediating targeted transport of OMV
BBa_25RK6MXG	PelB-Di-melittin	Di-melittin with pelB and C-terminal 6×His is a secreted, low-toxicity anti-inflammatory peptide.
BBa_25WWFI6X	BioPROTAC(VHH-VHL)	It mediates the targeted ubiquitination and proteasomal degradation of HIF-1α under hypoxia.
BBa_25HS8BFM	Cullin-2-6×His	A subunit of the RING-type E3 ubiquitin ligase complex, functions to recruit Rbx1.
BBa_255NFBXY	Rbx1-6×His	A subunit of the RING-type E3 ubiquitin ligase complex, catalyzes the transfer of ubiquitin from the E2 enzyme to the substrate.
BBa_25MPYP70	HIF1α (1-365 aa)-6×His	Used as substrate for in vitro ubiquination assay of bioPROTAC (VHH-VHL).
BBa_25BINJJU	EloB-6×His	A structural component of the RING-type E3 ubiquitin ligase complex.
BBa_25ASHOCU	EloC-6×His	A structural component of the RING-type E3 ubiquitin ligase complex.
BBa_256CDLTT	thiM-AmCyan	Used to characterize riboswitch.

Composite Parts Table

Part Number	Name	Part Description
BBa_25ZDQ5FO	Combination element of TPP riboswitch regulatory CcdB toxin protein	The component is mainly used to prevent engineered bacteria from escaping - the natural environment.
BBa_25IKITYX	SoxR/P_SoxS oxidative stress response promoter	Experience ROS and initiate Di-melittin expression
BBa_25U91GJF	QS-coupled TetR-mediated feedback inhibition element	Prevention of engineered bacterial overgrowth