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Human Practices

A Spark in the Haze

It began with a haze blurring the earth and sky. When our teammate Randolph returned home, he witnessed fields covered in smoke from burning straw—an issue often overlooked as a distant environmental problem suddenly became our reality. After rounds of online research, we learned that despite existing solutions like feed, fuel, or fertilizer, straw burning remains widespread. We asked a critical question: why? This compelled us to look beyond the smoke and engage directly with stakeholders, launching our Human Practices journey.

Figure 1. Burning straw observed by Randolph

Through this question-driven approach, we connected with farmers, experts, and partners, systematically analyzing their feedback using a structured Reflection Framework: clarifying our Purpose, capturing key Gains from each engagement, and translating insights into Expectations and Actions. These insights guided us through a 5-stage process, helping overcome key obstacles and shape milestones. Ultimately, this journey not only directed our implementation but also defined our core values: Shared Harvest, Resource Efficiency, Sustainability, Innovation, and Market Potential.

By deeply integrating stakeholder perspectives, we have developed a sustainable, farmer-centered solution with real-world applicability in the fashion industry. We share this work as an open and reproducible model, demonstrating how synthetic biology can address pressing ecological and socioeconomic challenges through thoughtful, inclusive, and iterative human practices.

Figure 2. Our Integrated Human Practices Paths
Note: 1. Stations mark our guiding questions. 2. Flags show milestones reached. 3. Click on any flag to explore our progress.

Milestone 1:
Straw is a major agricultural waste that poses handling challenges for farmers, often being burned—an unsustainable practice. While low-value uses like feed and fertilizer exist, consumer skepticism limits food-related applications. Supported by LINK SPIDER Co., Ltd., which specializes in sustainable fiber development, we decided to explore upgrading straw into higher-value textile fiber.

Milestone 2:
After receiving technical and market validation, we tried to produce sustainable textiles from agricultural waste-straws. Our attempt has gained the attention of potential customers. Besides, motivated by sustainability experts' critiques of traditional alkali methods, we will dedicate ourselves to creating a greener enzymatic pretreatment technology that minimizes environmental impact while maintaining processing efficiency.

Milestone 3:
We defined fashion manufacturers as our target customers. We established a business model covering sourcing, unmet market needs, spinning technology, and cost analysis, shaping our product roadmap.

Milestone 4:
We can enhance fiber strength by incorporating high-performance squid beak protein and Eumeta variegata silk fibroin. Our novel solution gains key recognition from our partners.

Milestone 5:
We raised awareness among consumers and sports sector experts through workshop, exhibition, and expert collaborations, promoting the value of sustainable textiles.

Our Guiding Compass

Values

Our Human Practices journey was a process of discovery that fundamentally shaped our project's identity. Through continuous engagement with our stakeholders, the insights we gathered resulted in the core values we hold today.

Shared Harvest

Creating new income for farmers by turning straw waste into high-value textile materials.

Resource Efficiency

Pursue the efficient transformation of low-value straw into high-performance, biodegradable fashion fibers.

Sustainability

Offering a fully biodegradable textile alternative that reduces land, water and chemical use.

Innovation

Using synthetic biology to design a novel straw-based material with enhanced properties.

Market Potential

Meeting growing global demand for sustainable textiles with scalable bio-based solutions.

Stakeholders' analysis



Reflection Framework

To ensure we could meaningfully learn from each interaction, we adopted a structured reflection framework. Based on the AREA framework, this "Purpose-Gains-Expectation & Action" cycle was essential in helping us distill raw feedback into the core principles that would go on to guide our project.

1. Purpose: This section is the goal we set before conducting each HP activity, serving as a guide to our IHP journey. We wish to gain different insights from different stakeholders to confirm the comprehensiveness of our project, making this section particularly important. A clear purpose ensures that we are collecting information that we need, while filtering out irrelevant voices.
2. Gains: This is the stage where we are actually engaging with our stakeholders through various forms, including but not limited to: questionnaires, interviews, small lectures, and visits. Through one-on-one engagements with our stakeholders, we collect the information that we need to improve on our project.
3. Expectation and Action: Based on the feedback received from each HP activity, we develop new expectations for our project. To meet these expectations, we take actions to perfect our project. As our project is very much human-centered, we pay extra attention to cater the needs of our stakeholders, making this sector indispensable.

Paths We Took

Our reflective framework structured a Question-Driven journey across 5 distinct stages. Each stage was initiated by a Guiding Question and ended with a key project Milestone, ensuring that stakeholder insights directly catalyzed our implementation. This iterative process of inquiry, engagement, and refinement allowed us to co-create the project with our community, transforming abstract challenges into real-world solutions.

Guiding Question 1: Is Agricultural Straw A significant Issue worth Addressing?

"The cost of baling and transporting the straw is higher than what we earn from selling it. For us, it's not an asset; it's a burden."

Rural farmer

To better understand the story behind billions of tons of agricultural waste, we communicated directly with its producers. Farmers confirmed that straw is a serious burden due to its low value, storage pressure, and inadequate reuse options—leaving burning as the default solution despite supportive policies.

"The key isn't more policies against burning—it's increasing the added value of by-product to improve its recycling."

Professor Xiaoqing Mu, Jiangnan University

Professor Mu pinpointed the root cause behind unused straw: products derived from it lack economic value. He urged us to identify higher-value applications, suggesting yeast-based protein as a compelling and commercially viable pathway.

"Fermentation could increase acidity in the feed, potentially leading to livestock acidosis."

Livestock farmer

When we tested the feed concept with livestock farmers, they questioned safety, efficacy, and demand—highlighting deep skepticism toward fermented feed and genetically modified production.

"Straw-derived cellulose presents a highly promising alternative to mitigate China’s dependency on imported dissolving pulp."

Eco-fabric manufacturer, Mr. Boxiang Wang

After pivoting away from feed, LINKS Spider’s CTO validated both the market demand and technical feasibility for straw-based textiles, emphasizing cost advantages and regulatory momentum.

Milestone 1:

Straw is a major agricultural waste that poses handling challenges for farmers, often being burned—an unsustainable practice. While low-value uses like feed and fertilizer exist, consumer skepticism limits food-related applications. Supported by LINK SPIDER Co., Ltd., which specializes in sustainable fiber development, we decided to explore upgrading straw into higher-value textile fiber.


Guiding Question 2: Can We Tap the Potential of Straws? Is Our Solution Practical?

"Straw-based fiber could significantly reduce China’s dependency on cotton and imported dissolving pulp."

CEO of FanRen Group, Ms. Lu

Speaking with FanRen’s sustainability-focused leadership confirmed severe shortages in both cotton and dissolving pulp. Ms. Lu emphasized that straw fiber must match mainstream materials in comfort, cost, and storytelling to succeed.

"Functionality—such as strength, elasticity, and durability—is as critical as sustainability for widespread adoption."

Staff from Esquel Group

A global manufacturing leader challenged the scarcity narrative, stressing that performance, cost, and consumer willingness to pay define adoption more than material shortages alone.

"I would pay more for clothing made from straw if it’s comfortable and backed by credible eco-certifications."

Potential consumers, questionnaire

A consumer survey exposed low awareness of straw fibers but strong willingness to pay a premium when comfort, price, and certifications align with expectations.

"Focus on specific applications like inner layers or UV-protective accessories where natural properties shine."

Sportswear designer, Biemlofen

A sportswear designer recommended targeting niche apparel categories first, pairing technical upgrades with authentic sustainability storytelling.

"Traditional alkali processing generates heavily polluted wastewater that undermines the environmental benefits of straw utilization."

Sustainability expert

An environmental expert scrutinized our value chain, urging us to address transport emissions, alkali wastewater, and certification to ensure genuine sustainability.

Milestone 2:

After receiving technical and market validation, we pursued sustainable textiles from agricultural straw and gained attention from potential customers. Motivated by sustainability experts’ critiques of traditional alkali methods, we committed to developing a greener enzymatic pretreatment that balances environmental impact with processing efficiency.


Guiding Question 3: How to Operate Our Venture?

"If recycling straw can bring us income, of course we're willing to do it – we just need collection points around the village."
— Female farmer, 62 years old, Fengkai County

Rural farmers

Interviews in Fengkai County revealed high straw output but limited reuse options. Farmers—many older women—showed strong willingness to recycle if given fair pricing, local collection, and job opportunities.

"Focus on serving business clients with stable performance and competitive costs—long-staple cotton is your benchmark."
— Mr. Wang, CTO of LINKS Spider

Eco-fabric manufacturer, Mr. Boxiang Wang

Mr. Wang provided commercialization guidance—advocating a B2B focus, Lyocell processing, and performance benchmarking—while offering spinning support through LINKS Spider’s pilot facility.

"After collecting agricultural waste, we will concentrate and inactivate it immediately, otherwise pests can grow."
— Julie, PEELSPHERE Designer

PEELSPHERE Designer, Ms. Julie

Julie shared how PEELSPHERE stabilizes agricultural residues, iterates product performance, and builds trust through certification and storytelling—insights we plan to adopt.

"Even a 1:1 blend ratio is progress. Straw fiber can gradually supplement wood pulp in Lyocell—you should focus on greener lignin removal and protein enhancement."

Materials engineer, Maoshuang Chen

Spinning trials at LINKS Spider revealed blending needs and highlighted the importance of greener pretreatment and protein-enhanced strengthening to reach textile-grade performance.

Milestone 3:

We defined fashion manufacturers as our target customers and established a business model covering sourcing, unmet market needs, spinning technology, and cost analysis—shaping our product roadmap.


Guiding Question 4: How to improve the performance of our fiber?

"Focus on the two selected proteins with CBM fusion—this approach balances performance gains with safety for your team."
— Mr. He, Secondary PI of 2025 LINKS-China

Our Secondary PI, Zhengyu He

Under our PI’s guidance, we shortlisted squid beak protein and Eumeta variegata silk fibroin—fused with CBMs—to safely enhance straw fibers within iGEM constraints.

"These protein-enhanced fibers show real potential for textile applications—the performance data speaks for itself."
— Maoshuang Chen, Materials Engineer, LINKS Spider

Materials Engineer, Maoshuang Chen

Spinning trials with 5% protein integration delivered 2–3× strength improvements, validating the industrial promise of protein-cellulose composites.

Milestone 4:

By integrating squid beak protein and Eumeta variegata silk fibroin, we demonstrated significant strength gains in straw-based fibers and earned recognition from industry partners.


Guiding Question 5: How can we amplify our impact?

"Learning that my clothing choices can help farmers and reduce pollution makes me feel empowered, not just eco-friendly."

Community, Interactive SynBio Workshops

Hands-on workshops reframed microorganisms as “magical helpers” for young participants, expanding awareness of sustainable textiles and inspiring future innovators.

"This is exactly what we need—materials that perform well while telling an authentic sustainability story."
— Julie, PEELSPHERE Designer

Julie, PEELSPHERE Designer (follow-up)

Sharing our first spun straw prototypes, Julie affirmed their quality and guided us on commercialization, certification, and storytelling for future market entry.

"If it's breathable, moves with me, and dries fast—and it's also sustainable—that's something I'd choose and my teammates would too."

Athletes

Interviews with athletes across sports highlighted performance priorities—breathability, elasticity, durability—and openness to paying more for sustainable gear that meets these demands.

"Art breathes new life into science. Transforming straw into art tells a new story of what can unfold when synthetic biology meets creativity—your project perfectly embodies our vision of life's continuous cycle."

Public art exhibition

Our partnership with Cyan Studio’s "Cycle" exhibition will showcase straw-based fibers as bioart, inviting public audiences to engage with sustainable materials.

Milestone 5:

Through workshops, exhibitions, and expert collaborations, we raised awareness among consumers and sports sector stakeholders, amplifying the value of sustainable textiles.

Fields to Future

Our Human Practices journey was never a separate chapter—it was the very framework that shaped our project's evolution. Every conversation directly influenced our engineering decisions: farmers' logistical challenges inspired our distributed recycling model; sustainability experts' critiques of traditional methods pushed us to develop our enzymatic pretreatment system; and feedback from eco-fabric manufacturers helped us improve our straw textile's performance. Even athletes' requirements guided the fiber enhancement protocol that made our material commercially viable.

This deep integration extends across every aspect of our work. The same stakeholder insights guided both our entrepreneurial strategy and our educational actions, where we shared the story of straw transformation with future scientists. Our inclusive approach with rural communities did more than create economic opportunities—it ensured our final product addressed real needs while upholding our core values.

We've documented this integrated approach through reusable templates, including a reflection framework that connects feedback to technical decisions and a stakeholder engagement map showing our journey from guiding questions to milestones. This documentation gives future teams a proven framework for projects where human needs and biological innovation advance together—showing that the most sustainable solutions come when we listen first and engineer second.