We encourage future iGEM teams to use our data, models, and parts for their own projects!

We verified the efficiency of the pAra promoter in C. Necator H16 and determined the optimal induction concentration: 2g/L Ara.

We also verified the effectiveness of hPDIA3 and SLY1 in promoting the soluble expression of human α-LA, which can help future teams solve the folding problem of expressing complex proteins in this chassis bacterium.

The competitive pathway genes we knocked out, which screened by dry experiment, provided a clear "road map" and a list of candidates for the subsequent team to transform the metabolic flow of C. Necator H16.

Any team that wants to engage in in-situ resource utilization (ISRU) or extreme environmental synthetic biology in the future can refer to it.

The whole process experience from molecule to system was provided from the selection of chassis, the optimization of gas fermentation conditions, to the design of microgravity bioreactor.

We used COBRA model to guide gene knockout, predict yield, and conduct sensitivity and robustness analysis, showing how to efficiently use computational tools to optimize wet experiment scheme for future teams, saving time and resources.

Our research on the biosafety basis of C. Necator H16 and the investigation of the public's acceptance of engineered bacteria food provide an important safety and ethical assessment framework for future teams dealing with similar non model bacteria or food related projects.

Strengthening the concept of "closed-loop system": the closed-loop manufacturing concept at the core of our project -- waste as a resource input -- is one of the ultimate goals for the sustainable development of synthetic biology. Our successful proof of concept will encourage future teams to consider the circularity and sustainability of the system more when designing projects.

The system can synthesize active α-LA in situ, avoid long-term storage and degradation of protein, and provide "fresh nutrition" for ultra long-term exploration such as Mars mission and Lunar base.

Through closed-loop circulation -- CO₂ comes from respiration, H₂ from electrolytic water, and nitrogen source from urine, there is no need to transport protein raw materials from the earth. Calculated by 180 days of Mars mission, it can save more than $3.6 million in transportation costs and reduce space waste generation.

The closed-loop logic of the project is not only applicable to space, but also to other extreme environment of the earth, such as polar scientific research, deep-sea exploration, post disaster rescue, etc.

Breaking the circle of popular science -- turning "advanced aerospace" into "touchable knowledge":

We corporate with community to organize a science-themed party. We also designed several games to convey the knowledge of hydrogen bacteria metabolism and α-LA function through the links of good luck grid, quick questions and answers, and continuous watching. The technical details of the space in situ protein manufacturing system will be introduced in the subsequent mass entrepreneurship and innovation lectures using games as the bedding.